LOCUS       sequence-346172-        5184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) 
            POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal 
            epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5184)
  TITLE     pENTR plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) 
            POLYMERASE 1 (PARP1) without stop codon for C-terminal epitope 
            tagging
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR4 CDS A. thaliana PARP1
FEATURES             Location/Qualifiers
     source          1..5184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      31..58
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     66..89
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    108..207
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3163..3262)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3374..3393)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3385..4194
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3452..3471)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4773..4792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      5037..5123
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg
       61 cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa ataatgattt
      121 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc ttttttataa
      181 tgccaacttt gtacaaaaaa gcaggctcca ccatggcaag cccacataag ccgtggaggg
      241 cggagtatgc aaagtcgtcg aggtcttcat gtaaaacttg caagtccgtc attaacaagg
      301 agaactttcg tcttggaaag ttggttcaat ctactcactt cgatggcatc atgcccatgt
      361 ggaaccatgc ttcttgtata ctaaagaaga cgaagcagat aaaatcagtt gatgatgttg
      421 aaggcataga atcacttcgt tgggaagatc agcaaaagat tagaaaatat gtcgaatctg
      481 gagcagggag taacacaagc acaagcacag gcacaagcac gagcagtacc gctaataatg
      541 ctaaactaga atatgggatt gaagtgtcac aaacttctcg tgccggttgc agaaagtgta
      601 gcgaaaagat cttgaaagga gaggtacgta tattctccaa gcctgaaggc ccgggtaaca
      661 aaggtttgat gtggcatcac gctaaatgtt tccttgaaat gtcttcctct actgaactgg
      721 aaagtttgtc tggatggaga agtataccag actcagacca agaagctctt cttcccttag
      781 tgaagaaagc tctgccggca gccaaaactg agacagcaga agcacgtcaa acaaattcaa
      841 gagcaggcac aaaacgaaaa aatgattctg ttgataacga gaagtcgaaa ctagcaaaaa
      901 gtagttttga catgtctaca agtggtgctt tacaaccttg tagcaaagaa aaggaaatgg
      961 aggcccaaac taaggaattg tgggacctga aggatgatct gaaaaaatat gtaacatcag
     1021 ctgagttgcg ggaaatgctt gaagtaaatg aacaaagtac aagaggatct gaacttgatc
     1081 tgcgtgataa atgtgctgat ggcatgatgt ttggcccact cgctctctgc ccaatgtgct
     1141 ctgggcatct ttctttctcc ggaggacttt accgatgcca tggatacatc tcagaatgga
     1201 gcaaatgttc tcattccact ttggatccag accgcatcaa agggaagtgg aaaatccctg
     1261 acgaaacaga aaatcaattc cttctgaagt ggaataagtc tcaaaagagt gtgaagccaa
     1321 aacgtattct gcgtcctgta ttgtctggcg agacatctca gggtcaaggt tctaaagatg
     1381 caactgactc ctcaaggagt gaaaggctag cagatcttaa agtttcaatt gctggaaata
     1441 ctaaggaaag gcaaccatgg aagaagagaa ttgaggaagc tggtgcagag tttcatgcta
     1501 atgttaaaaa aggtacaagc tgtttggttg tttgtggact gacagatatc agagacgctg
     1561 aaatgagaaa ggcaaggagg atgaaagtgg caatcgtgag agaggattat ttggttgatt
     1621 gttttaaaaa acagaggaaa cttccatttg acaagtacaa aattgaagac actagtgaga
     1681 gccttgtcac tgttaaagta aaaggacgaa gcgctgtgca tgaagcgtct ggcctccaag
     1741 agcactgtca cattcttgaa gatgggaaca gtatctataa cacaactctg agcatgtctg
     1801 atctctctac cggtatcaat agttattaca tactccagat aatccaagaa gataaaggtt
     1861 cagattgtta cgtatttcgt aaatggggcc gagttggaaa tgaaaagatt ggtggtaaca
     1921 aagtggagga aatgtcaaag tctgatgcgg ttcacgaatt caaacgtcta tttcttgaaa
     1981 aaaccggaaa cacatgggaa tcttgggaac aaaaaacgaa tttccagaaa caacctggaa
     2041 aatttctccc gttggacatt gattatggag ttaataagca agtagccaaa aaagagccat
     2101 ttcagaccag tagcaacctt gctccatcat taatagaatt gatgaagatg ctttttgatg
     2161 tggaaactta cagatctgca atgatggagt tcgagataaa tatgtcagag atgccacttg
     2221 ggaagctcag caaacataat atacagaagg gttttgaggc attgacggag atacagaggc
     2281 tattgactga aagcgacccc cagcctacta tgaaagaaag cttgcttgtt gatgctagta
     2341 acagattttt taccatgatc ccttctattc atcctcatat tatccgagat gaagatgact
     2401 ttaagtcaaa ggtgaaaatg ctcgaggctc tgcaggatat cgaaatagct tcaagaatag
     2461 ttggctttga tgttgatagc accgaatctc ttgatgataa gtataagaag ctgcattgcg
     2521 atatctcacc acttcctcat gatagcgaag attatcgatt aatcgagaag tatcttaaca
     2581 caactcatgc cccaacgcat acagagtgga gtcttgagct agaggaagtt tttgctcttg
     2641 aaagagaagg agagtttgat aaatatgctc cccacagaga aaaacttggc aataagatgc
     2701 tcctatggca tggttctcga ttaacgaatt ttgttggaat attgaaccaa ggactgagaa
     2761 ttgcacctcc agaagctcct gctactggtt acatgtttgg aaaagggata tactttgctg
     2821 accttgtcag taaaagtgct cagtactgct acacttgtaa gaaaaatccg gtgggtctaa
     2881 tgcttctgag tgaagttgca ttgggagaaa tacatgagct aacaaaagct aagtatatgg
     2941 ataaacctcc gagagggaaa cactcgacca aagggctcgg caagaaagtg cctcaagatt
     3001 ccgagtttgc caagtggaga ggtgatgtga ctgttccctg tggaaaacct gtttcatcaa
     3061 aggtcaaggc ttctgagctt atgtacaatg agtatatcgt ctacgataca gcccaggtga
     3121 agttgcagtt cttgttgaaa gtaaggttta agcacaagag agacccagct ttcttgtaca
     3181 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3241 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc
     3301 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     3361 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     3421 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     3481 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     3541 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     3601 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tggtgatgca
     3661 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     3721 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3781 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3841 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3901 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3961 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     4021 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     4081 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     4141 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     4201 ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc gtcagacccc
     4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct
     4981 agcatggatc tcggggacgt ctaactacta agcgagagta gggaactgcc aggcatcaaa
     5041 taaaacgaaa ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga
     5101 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc
     5161 ccggagggtg gcgggcagga cgcc
//
