LOCUS       sequence-34621-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MUM1
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             516..533
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(586..604)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      600..647
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcgaacca cgttcgtttg aagtaggaat gctagtctgg
      121 cataaacata aaaaataccc cttctggcca gcagtggtca aaagcgtcag gcagagagat
      181 aagaaagcaa gtgtgctata catcgaagga cacatgaacc cgaaaatgaa aggtttcaca
      241 gtgtctctta aaagtttaaa gcactttgat tgtaaagaga aacagacgct tctgaatcaa
      301 gccagggagg acttcaacca ggacatcggc tggtgtgtct ccctcatcac cgactacagg
      361 gtccggttag gctgcgggtc ttttgctggc tctttcctgg aatattacgc ggctgatata
      421 agctatcctg tccgaaaatc catccagcag gacgtcttgg ggaccaagct tcctcaactg
      481 agcaagtgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      541 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      601 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      661 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      841 gctcccttta gggt
//
