LOCUS       sequence-346288-       10783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Focal Adhesion marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Arts JJG, Mahlandt EK, Schimmel L, Gronloh MLB, van der Niet S, 
            Klein BJAM, Fernandez-Borja M, van Geemen D, Huveneers S, van 
            Rijssel J, Goedhart J, van Buul JD
  TITLE     Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes 
            During Basolateral Crawling.
  JOURNAL   Front Immunol. 2021 May 18;12:667213. doi: 
            10.3389/fimmu.2021.667213. eCollection 2021.
  PUBMED    34084168
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2021-05-18"; volume: "12:667213. doi"; pages: " 
            10.3389/fimmu.2021.667213. eCollection 2021"
COMMENT     Sequence Label: mNeonGreen-Paxillin
FEATURES             Location/Qualifiers
     source          1..10783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      687..696
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             693..1400
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      948..957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3301..3853
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3445..3462)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3672..3691
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3880..4479
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3880..3899)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4376..4396
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5371..5604
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5676..5797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5713..5732)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5767..5786
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5837..5972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5992..6011)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5993..6011)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6021..6038)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6021..6037)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6030..6052)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6179..6634
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6266..6285)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6476..6497
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6660..6764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6765..6833,6834..7625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6983..7002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7796..8384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8285..8304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8538..8555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8672..8693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8708..8713,8714..8731,8732..8738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8746..8762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8751..8773
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8770..8786
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8770..8786
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8805..8825
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8807..8825
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8853..9079
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9080..9260
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9307..9432
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9925..10158
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10343..10387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10649..10766
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagct agaggatccc
      601 ccgggctgca ggaattcggc gcgccacgcg taccgatatc cagcacagtg gcggccgctc
      661 gagcctctag acagcgctca ccggtcgcca ccatggtgag caagggcgag gaggataaca
      721 tggcctctct cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact
      781 ttgacatggt gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga
      841 agtccaccaa gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg
      901 gcttccatca gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag
      961 atggctccgg ctaccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg
     1021 ttaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga
     1081 ctggtttccc tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca
     1141 ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca
     1201 ccactggaaa tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc
     1261 caatggcggc taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca
     1321 agcactccaa gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg
     1381 gcatggacga gctgtacaag tccggactca gatctcgagc tgcggccgcc ggatccaagc
     1441 ttatggacga cctcgacgcc ctgctggcgg acttggagtc taccacctcc cacatctcca
     1501 aacggcctgt gttcttgtcg gaggagaccc cctactcata cccaactgga aaccacacat
     1561 accaggagat tgccgtgcca ccccccgtcc ccccaccccc gtccagcgag gccctcaatg
     1621 gcacaatcct tgacccctta gaccagtggc agcccagcgg ctcccgattc atccaccagc
     1681 agcctcagtc ctcatcacct gtgtacggct ccagtgccaa aacttccagt gtctccaacc
     1741 ctcaggacag tgttggctct ccgtgctccc gagtgggtga ggaggagcac gtctacagct
     1801 tccccaacaa gcagaaatca gctgagcctt cacccaccgt aatgagcacg tccctgggca
     1861 gcaacctttc tgaactcgac cgcctgctgc tggaactgaa cgctgtacag cataacccgc
     1921 caggcttccc tgcagatgag gccaactcaa gccccccgct tcctggggcc ctgagccccc
     1981 tctatggtgt cccagagact aacagcccct tgggaggcaa agctgggccc ctgacgaaag
     2041 agaagcctaa gcggaatggg ggccggggcc tggaggacgt gcggcccagt gtggagagtc
     2101 tcttggatga actggagagc tccgtgccca gccccgtccc tgccatcact gtgaaccagg
     2161 gcgagatgag cagcccgcag cgcgtcacct ccacccaaca gcagacacgc atctcggcct
     2221 cctctgccac cagggagctg gacgagctga tggcttcgct gtcggatttc aagttcatgg
     2281 cccaggggaa gacagggagc agctcacccc ctggggggcc cccgaagccc gggagccagc
     2341 tggacagcat gctggggagc ctgcagtctg acctgaacaa gctgggggtc gccacagtcg
     2401 ccaaaggagt ctgcggggcc tgcaagaagc ccatcgccgg gcaggttgtg accgccatgg
     2461 ggaagacgtg gcaccccgag cacttcgtct gcacccactg ccaggaggag atcggatccc
     2521 ggaacttctt cgagcgggat ggacagccct actgtgaaaa ggactaccac aacctcttct
     2581 ccccgcgctg ctactactgc aacggcccca tcctggataa agtggtgaca gcccttgacc
     2641 ggacgtggca ccctgaacac ttcttctgtg cacagtgtgg agccttcttt ggtcccgaag
     2701 ggttccacga gaaggacggc aaggcctact gtcgcaagga ctacttcgac atgttcgcac
     2761 ccaagtgtgg cggctgcgcc cgggccatcc tggagaacta tatctcagcc ctcaacacgc
     2821 tgtggcatcc tgagtgcttt gtgtgccggg aatgcttcac gccattcgtg aacggcagct
     2881 tcttcgagca cgacgggcag ccctactgtg aggtgcacta ccacgagcgg cgcggctcgc
     2941 tgtgttctgg ctgccagaag cccatcaccg gccgctgcat caccgccatg gccaagaagt
     3001 tccaccccga gcacttcgtc tgtgccttct gcctcaagca gctcaacaag ggcaccttca
     3061 aggagcagaa cgacaagcct tactgtcaga actgcttcct caagctcttc tgctaggcta
     3121 gcctagtgcc atttgttcag tggttcgtag ggctttcccc cactgtttgg ctttcagtta
     3181 tatggatgat gtggtattgg gggccaagtc tgtacagcat cttgagtccc tttttaccgc
     3241 tgttaccaat tttcttttgt ctttgggtaa acccgggaat tccgcccctc tcccccccta
     3301 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3361 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3421 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3481 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3541 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     3601 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     3661 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3721 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3781 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3841 cacgatgata atatggatat ggccacaacg gtcgccacca tgaccgagta caagcccacg
     3901 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     3961 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     4021 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4081 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4141 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4201 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4261 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4321 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4381 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4441 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     4501 cgcccgaccg aaaggagcgc acgaccccat gcatgtattc aatctaagca ggctttcact
     4561 ttctcgccaa cttacaaggc ctttctgtgt aaacaatacc tgaaccttta ccccgttgcc
     4621 cggcaacggc caggtctgtg ccaagtgttt gctgacgcaa cccccactgg ctggggcttg
     4681 gtcatgggcc atcagcgcat gcgtggaacc ttttcggctc ctctgccgat ccatactgcg
     4741 gaactcctag ccgcttgttt tgctcgcagc aggtctggag caaacattat cgggactgat
     4801 aactctgttg tcctatcccg caaatataca tcgtttccat ggctgctagg ctgtgctgcc
     4861 aactggatcc tgcgcgggac gtcctttgtt tacgtcccgt cggcgctgaa tcctgcggac
     4921 gacccttctc ggggtcgctt gggactctct cgtccccttc tccgtctgcc gttccgaccg
     4981 accacggggc gcacctctct ttacgcggac tccccgtctg tgccttctca tctgccggac
     5041 cgtgtgcact tcgcttcacc tctgcacgtc gcatggagac caccgtgaac gcccaccaaa
     5101 tattgcccaa ggtcttacat aagaggactc ttggactctc agcaatgtca acgaccgacc
     5161 ttgaggcata cttcaaagac tgtttgttta aagactggga ggagttgggg gaggagatta
     5221 ggttaaaggt ctttgtacta ggaggctgta ggcataaatt ggtctgcgca ccagcaccat
     5281 ggcgcaatca ctagagcggg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5341 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5401 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5461 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5521 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5581 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5641 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5701 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5761 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5821 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5881 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5941 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6001 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6061 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6181 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6241 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6301 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6361 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6421 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6481 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6541 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6601 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     6661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6841 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6901 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6961 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7021 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7081 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7141 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7201 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7261 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7321 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7381 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7441 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7501 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7561 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7621 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7681 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7921 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8161 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8221 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8281 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8341 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8401 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8461 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8521 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8581 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8641 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8701 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8761 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8821 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     8881 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     8941 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     9001 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     9061 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     9121 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9181 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9241 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9301 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9361 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9421 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9481 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9541 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9601 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9661 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9721 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     9781 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9841 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9901 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9961 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    10021 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    10081 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    10141 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    10201 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10261 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10321 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10381 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10441 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    10501 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    10561 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    10621 attagtgaac ggatctcgac ggtatcggtt ttaaaagaaa aggggggatt ggggggtaca
    10681 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10741 aacaaattac aaaaattcaa aattttatcg ataagcttgg gag
//
