LOCUS       sequence-346384-        9745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3124
FEATURES             Location/Qualifiers
     source          1..9745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2499..2523)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2531..2572
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2683..3271
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2736..2756)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3154..3165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3343..3576
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3654..3775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3691..3710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3745..3764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3815..3950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3970..3989)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3971..3989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3999..4016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3999..4015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4008..4030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4157..4612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4244..4263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4454..4475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4638..4742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4743..4811,4812..5603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4961..4980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5774..6362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6263..6282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6516..6533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6650..6671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6686..6691,6692..6709,6710..6716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6724..6740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6729..6751
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6748..6764
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6748..6764
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6783..6803
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6785..6803
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6831..7057
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7058..7238
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7285..7410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7903..8136
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8321..8365
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8596..8806
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8618..8814
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8665..8800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8846..9448
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8846..8865)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9342..9362
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9491..9608
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtcgtc catcctgcct ttcactcccc cgatcgtgaa
     1261 gcgcctgctg ggctggaaga agggcgagca gaacgggcag gaggagaaat ggtgcgagaa
     1321 ggcggtcaag agcctggtca agaaactcaa gaagacgggg cagctggacg agctggagaa
     1381 ggccatcacc acgcagaacg tcaacaccaa gtgcatcacc atccccaggt ccctggatgg
     1441 ccggttgcag gtgtcccatc ggaaggggct ccctcatgtc atctactgcc gcctgtggcg
     1501 atggccagac ctgcacagcc accacgagct gcgggccatg gagctgtgtg agttcgcctt
     1561 caatatgaag aaggacgagg tctgcgtgaa tccctaccac taccagagag tagagacacc
     1621 agttctacct cctgtgttgg tgccacgcca cacagagatc ccggccgagt tccccccact
     1681 ggacgactac agccattcca tccccgaaaa cactaacttc cccgcaggca tcgagcccca
     1741 gagcaatatt ccagagaccc caccccctgg ctacctgagt gaagatggag aaaccagtga
     1801 ccaccagatg aaccacagca tggacgcagg ttctccaaac ctatccccga atccgatgtc
     1861 cccagcacat aataacttgg acctgcagcc agttacctac tgcgagccgg ccttctggtg
     1921 ctccatctcc tactacgagc tgaaccagcg cgtcggggag acattccacg cctcgcagcc
     1981 atccatgact gtggatggct tcaccgaccc ctccaattcg gagcgcttct gcctagggct
     2041 gctctccaat gtcaacagga atgcagcagt ggagctgaca cggagacaca tcggaagagg
     2101 cgtgcggctc tactacatcg gaggggaggt cttcgcagag tgcctcagtg acagcgctat
     2161 ttttgtccag tctcccaact gtaaccagcg ctatggctgg cacccggcca ccgtctgcaa
     2221 gatcccacca ggatgcaacc tgaagatctt caacaaccag gagttcgctg ccctcctggc
     2281 ccagtcggtc aaccagggct ttgaggctgt ctaccagttg acccgaatgt gcaccatccg
     2341 catgagcttc gtcaaaggct ggggagcgga gtacaggaga cagactgtga ccagtacccc
     2401 ctgctggatt gagctgcacc tgaatgggcc tttgcagtgg cttgacaagg tcctcaccca
     2461 gatgggctcc ccaagcatcc gctgttccag tgtgtcttac ccaactttct tgtacaaagt
     2521 ggttgatatc ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
     2581 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2641 cgacaaactt ggttggcatg caaagatacg cgttaagtcg acaatcaacc tctggattac
     2701 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2761 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2821 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2881 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2941 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3001 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3061 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3121 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3181 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3241 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     3301 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3361 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3421 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3481 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3541 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     3601 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     3661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3721 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3781 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     3961 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     4021 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4081 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4141 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4201 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4261 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4321 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4381 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4441 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4501 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4561 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     4621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4741 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4801 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4861 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4921 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4981 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5041 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5101 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5161 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5221 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5281 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5341 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5401 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5461 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5521 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5581 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5641 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5701 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5761 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5821 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5881 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5941 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6001 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6061 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6121 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6181 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6241 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6301 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6361 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6421 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6481 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6541 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6601 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6661 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6721 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6781 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6841 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6901 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6961 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     7021 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     7081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     7381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7741 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7801 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7861 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7921 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7981 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8041 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8101 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8161 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8221 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8281 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8341 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8401 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8461 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8521 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     8581 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     8641 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8701 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8761 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     8821 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     8881 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8941 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     9001 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     9061 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     9121 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     9181 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     9241 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     9301 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     9361 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     9421 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
     9481 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9541 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9601 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
     9661 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     9721 ctaggtcttg aaaggagtgg gaatt
//
