LOCUS       sequence-346386-        9997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3126
FEATURES             Location/Qualifiers
     source          1..9997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2751..2775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2783..2824
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2935..3523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2988..3008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3406..3417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3595..3828
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3906..4027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3943..3962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3997..4016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4067..4202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4222..4241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4223..4241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4251..4268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4251..4267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4260..4282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4409..4864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4496..4515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4706..4727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4890..4994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4995..5063,5064..5855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5213..5232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6026..6614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6515..6534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6768..6785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6902..6923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6938..6943,6944..6961,6962..6968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6976..6992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6981..7003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7000..7016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7000..7016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7035..7055
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7037..7055
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7083..7309
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7310..7490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7537..7662
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8155..8388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8573..8617
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8848..9058
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8870..9066
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8917..9052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9098..9700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9098..9117)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9594..9614
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9743..9860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggaaga tatacaaaca aatgcggaac tgaaaagcac
     1261 tcaggagcag tctgtgcccg cagaaagtgc agcggttttg aatgactaca gtttaaccaa
     1321 atctcatgaa atggaaaatg tggacagtgg agaaggccca gccaatgaag atgaagacat
     1381 aggagatgat tcaatgaaag tgaaagatga atacagtgaa agagatgaga atgttttaaa
     1441 gtcagaaccc atgggaaatg cagaagagcc tgaaatccct tacagctatt caagagaata
     1501 taatgaatat gaaaacatta agttggagag acatgttgtc tcattcgata gtagcaggcc
     1561 aaccagtgga aagatgaact gcgatgtgtg tggattatcc tgcatcagct tcaatgtctt
     1621 aatggttcat aagcgaagcc atactggtga acgcccattc cagtgtaatc agtgtggggc
     1681 atcttttact cagaaaggta acctcctccg ccacattaaa ctgcacacag gggaaaaacc
     1741 ttttaagtgt cacctctgca actatgcatg ccaaagaaga gatgcgctca cggggcatct
     1801 taggacacat tctgtggaga aaccctacaa atgtgagttt tgtggaagga gttacaagca
     1861 gagaagttcc cttgaggagc acaaggagcg ctgccgtaca tttcttcaga gcactgaccc
     1921 aggggacact gcaagtgcgg aggcaagaca catcaaagca gagatgggaa gtgaaagagc
     1981 tctcgtactg gacagattag caagcaatgt ggcaaaacga aaaagctcaa tgcctcagaa
     2041 attcattggt gagaagcgcc actgctttga tgtcaactat aattcaagtt acatgtatga
     2101 gaaagagagt gagctcatac agacccgcat gatggaccaa gccatcaata acgccatcag
     2161 ctatcttggc gccgaagccc tgcgcccctt ggtccagaca ccgcctgctc ccacctcgga
     2221 gatggttcca gttatcagca gcatgtatcc catagccctc acccgggctg agatgtcaaa
     2281 cggtgcccct caagagctgg aaaagaaaag catccacctt ccagagaaga gcgtgccttc
     2341 tgagagaggc ctctctccca acaatagtgg ccacgactcc acggacactg acagcaacca
     2401 tgaagaacgc cagaatcaca tctatcagca aaatcacatg gtcctgtctc gggcccgcaa
     2461 tgggatgcca cttctgaagg aggttccccg ctcttacgaa ctcctcaagc ccccgcccat
     2521 ctgcccaaga gactccgtca aagtgatcaa caaggaaggg gaggtgatgg atgtgtatcg
     2581 gtgtgaccac tgccgcgtcc tcttcctgga ctatgtgatg ttcacgattc acatgggctg
     2641 ccacggcttc cgtgaccctt tcgagtgtaa catgtgtgga tatcgaagcc atgatcggta
     2701 tgagttctcg tctcacatag ccagaggaga acacagagcc ctgctgaagt gcccaacttt
     2761 cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc
     2821 tacgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2881 ttgtggaaag gacgaggata catcctataa tgtgcattaa cgcgttaagt cgacaatcaa
     2941 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3001 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3061 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3121 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3181 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3241 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3301 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3361 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3421 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3481 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3541 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3601 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3841 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3901 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4021 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     4081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4141 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4201 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4261 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4321 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4381 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4441 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4501 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4561 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4621 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4681 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4741 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4801 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4861 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6901 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6961 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7021 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     7081 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7141 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7201 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7261 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7321 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7381 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7441 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7501 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7561 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7621 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7681 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7741 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7801 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7861 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7921 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7981 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8041 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8101 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8161 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8221 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8281 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8341 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8401 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8461 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8521 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8581 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8641 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8701 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8761 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8821 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8881 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8941 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9001 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9061 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     9121 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     9181 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     9241 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9301 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9361 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9421 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9481 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9541 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9601 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9661 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9721 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9781 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9841 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9901 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9961 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
