LOCUS       sequence-346616-       10293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH 
            (1-140D) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 SKICH (1-140D)
FEATURES             Location/Qualifiers
     source          1..10293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     327..350
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    404..428
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2382..2406)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2422..3132,3133..3135,3136..3138))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2422..3132,3133..3135,3136..3138))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2464..2485)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2813..2832
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3072..3093
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3135..3144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3203..3227)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3224..3427)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3253..3273)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3428..3731
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3783..3900
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4193..4237)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4422..4655
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5152..5277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5324..5957
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6218..6234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6218..6234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6231..6253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6242..6258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6266..6272,6273..6290,6291..6296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6311..6332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6572..6589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6743..7331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6823..6842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7502..8293,8294..8362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8125..8144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8363..8467)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8535..8553
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             8672..8905
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8980..9568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9086..9097
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9495..9515
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    9594..9715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9659..9678
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9757..10155)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(join(10179..10293,1..385))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10227..10246)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
       61 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
      121 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg
      181 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
      241 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
      301 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
      361 cttgggaaaa gcgcctcccc tacccggtag aattcgttat cgaaccactt tgtacaagaa
      421 agttgggtag tcaaaattta gaacattctg atcaaaatgg gtctgcacat gcctttcaaa
      481 cacctgctgg tcatagtcag gagggaactg ctcgctgcac atcgggcaca ccttccagtg
      541 actttcaaca tgttcttcaa atttgctctg atcatagtta ggaggaaaca ttaactcaca
      601 gaggggacac ttcttgtgaa catcaaagct ggaatcaaag caaaagcctg tcccatgccc
      661 acgtaaatgt tgacttggag gatcaggagc agtaggcaca ttctcatctt ctttgctatc
      721 ctcagagtcc tctaagccat caggccgact aaagtttcga gcaggctggc tgcagacaac
      781 attgtcttcc agtccccaag aggggactct gacaggtggc ctttgtattt catctgggta
      841 aaaagcacca tctgctccat ctcttgtttc ctgagatgca taaggattac catattgcag
      901 aactggttgt gcatttgaca atgtgagaac ataaccattt tgctctgagc atgttttgaa
      961 acattgagga ctctgggaat tctgaccttc cattttcctt tctgctggat tttctagact
     1021 ccttttaagt tctttataat tgtcctgtac ttcagctagt tcaagtttta cattttcagc
     1081 aattttcact tgttctttcc atttcagctc catttttgca agttcatcag catatttatt
     1141 gcattttgct ttctcatcct gcaagagttg tttacattta ttatactttt ctgttttgtc
     1201 agcaatttct ttggtcattt cacagacttg cccctttgtt actatgtcaa aatctgcctc
     1261 tgctgcagaa ggtgatggct ttacatctac agtagaggca gaagtggctg ggtctgtgtt
     1321 tactgaagca tcattcactt tctgctgatt ccccgttttc tttgtgaaga cattattatt
     1381 attagctgat tgatctgaaa gtttgtttat ttgtttttgg agcctttggc attccttaaa
     1441 tttttcttta taatggtctg cagccatctg aagacggagt ttcagatctt caacttctct
     1501 tcttagttcg tgttccagtg tatcagtctt gtcctgatct tttttcatag catttagttt
     1561 aagttctgcc actgcatcag ctaactgctt tttcactttc tcgttttcca agcgtgcagt
     1621 atgcaggtct gccatcgttc tgtctcgtac gttgacagca tcactgagtt ctttagccag
     1681 aaagacaact tcttgccgag ttgcctgaac ctgttcctct gctttacgaa gttgctcctt
     1741 taaaaaacaa gtatcttctt tacttgaggt tgtaagcagg aaagttcttt gcagattttc
     1801 cttttcagcc aaacataact gcagcctgcc aatctcttct ttgaaatgag taatcacgct
     1861 ttctttgttc ccatctaaat tttttaaagt ctggacctct gacataagct tggtattttc
     1921 tatttctgta ttcttcaaat gtaccttata aagttccttt tcatccttct ctgtcttcaa
     1981 ctgacattca agttgttctc tttcatgttg tgccttcttg agtttgtcct ttaaactgtc
     2041 taattcggtt tctttttcaa ttgctttatg tgttactgac acaatatctt cctcaagctg
     2101 atgggctttg gatgtagcat cactgaacct cttcttaaac tcttcatttt ccatttttaa
     2161 gctttgtgtt acttcagtaa gacccttttg ttctgcttgc agttggtcac atctttcttt
     2221 ctcatggtta agttctcttt ccattctccc aacttgttct cgaagttgtg ctgtttcttt
     2281 ttccagaacg gcaattaact ttaacagttc ttctttttct ttcatggttt tctcaatttt
     2341 caactcaaga aggcctgctt ttgtggtcac cactaacatg gtgccagctt ttttgtacaa
     2401 acttgtttga taacctcgag ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     2461 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     2521 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     2581 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     2641 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     2701 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     2761 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     2821 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     2881 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     2941 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     3001 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     3061 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     3121 ctcgcccttg ctcaccatgg tggcagatcg gccgccggtg tcttctatgg aggtcaaaac
     3181 agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga
     3241 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     3301 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     3361 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     3421 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     3481 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     3541 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     3601 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     3661 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     3721 gttatgtaac gcggaactcc actagtccaa actggatctc tgctgtccct gtaataaacc
     3781 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     3841 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     3901 ttgtggatga atactgccat ttgtgaattc ggcgataccg tcgagatccg ttcactaatc
     3961 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     4021 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     4081 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     4141 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     4201 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     4261 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     4321 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     4381 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     4441 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     4501 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     4561 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     4621 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     4681 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     4741 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     4801 tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     4861 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     4921 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     4981 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     5041 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     5101 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     5161 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     5221 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     5281 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     5341 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     5401 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     5461 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
     5521 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
     5581 ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa
     5641 gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca
     5701 ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct
     5761 ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta
     5821 gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc
     5881 agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga
     5941 gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata caatttcttt
     6001 aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac aggtaattgg
     6061 cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct tgagttatcc
     6121 agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6181 gtgaaaaaaa tgctttattt gtgaaattcg taatcatgtc atagctgttt cctgtgtgaa
     6241 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6301 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6361 agtcgggaaa cctgtcgtgc cacggggagc tttttgcaaa agcctaggcc tccaaaaaag
     6421 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     6481 aaaaaaatta gtcagccatg agcttgctgc attaatgaat cggccaacgc gcggggagag
     6541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7141 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8101 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8461 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     8521 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa tgatctagcc
     8581 ctttccttaa ttaacccggg cctctcactc tctgatattc atttctttgc aagttataaa
     8641 tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag
     8701 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     8761 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     8821 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     8881 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     8941 acagctgcct tgtaagtcat tggtcttaaa ggatctcagg cggggaggcg gcccaaaggg
     9001 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     9061 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     9121 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     9181 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     9241 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     9301 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     9361 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     9421 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     9481 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     9541 cacaaatttt gtaatccaga ggttgattag gatctatcga tgcatagaca tgataagata
     9601 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     9661 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagtttcgag
     9721 gtcgagtgtc agtcctgctc ctcggccacg aagtgcttag ccctcccaca cataaccaga
     9781 gggcagcaat tcacgaatcc caactgccgt cggctgtcca tcactgtcct tcactatggc
     9841 tttgatccca ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat
     9901 gcccctgttc tcatttccga tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc
     9961 agcagtgccc agcaccacga gttctgcaca aggtccccca gtaaaatgat atacattgac
    10021 accagtgaag atgcggccgt cgctagagag agctgcgctg gcgacgctgt agtcttcaga
    10081 gatggggatg ctgttgattg tagccgttgc tctttcaatg agggtggatt cttcttgaga
    10141 caaaggcttg gccatggttt aattaatggg ctgcaggtcg aaaggcccgg agatgaggaa
    10201 gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag
    10261 gaccttcggg cgcccgcccc gcccctgagc ccg
//
