LOCUS       sequence-346804-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3137
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2154..2178)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2186..2227
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2338..2926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2391..2411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2809..2820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2998..3231
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3309..3430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3346..3365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3400..3419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3470..3605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3625..3644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3626..3644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3654..3671)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3654..3670)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3663..3685)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3812..4267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3899..3918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4109..4130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4293..4397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4398..4466,4467..5258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4616..4635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5429..6017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5918..5937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6171..6188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6305..6326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6341..6346,6347..6364,6365..6371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6379..6395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6384..6406
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6403..6419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6403..6419
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6438..6458
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6440..6458
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6486..6712
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6713..6893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6940..7065
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7558..7791
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7976..8020
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8251..8461
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8273..8469
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8320..8455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8501..9103
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8501..8520)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8997..9017
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9146..9263
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgaa gcgagctcac cccgagtaca gctcctcgga
     1261 cagcgagctg gacgagacca tcgaggtgga gaaggagagt gcggacgaga atggaaactt
     1321 gagttcggct ctaggttcca tgtccccaac tacatcttcc cagattttgg ccagaaaaag
     1381 acggagagga ataattgaga agcgccgacg agaccggatc aataacagtt tgtctgagct
     1441 gagaaggctg gtacccagtg cttttgagaa gcaggtaatg gagcaaggat ctgctaagct
     1501 agaaaaagcc gagatcctgc agatgaccgt ggatcacctg aaaatgctgc atacggcagg
     1561 agggaaaggt tactttgacg cgcacgccct tgctatggac tatcggagtt tgggatttcg
     1621 ggaatgcctg gcagaagttg cgcgttatct gagcatcatt gaaggactag atgcctctga
     1681 cccgcttcga gttcgactgg tttcgcatct caacaactac gcttcccagc gggaagccgc
     1741 gagcggcgcc cacgcgggcc tcggacacat tccctggggg accgtcttcg gacatcaccc
     1801 gcacatcgcg cacccgctgt tgctgcccca gaacggccac gggaacgcgg gcaccacggc
     1861 ctcacccacg gaaccgcacc accagggcag gctgggctcg gcacatccgg aggcgcctgc
     1921 tttgcgagcg ccccctagcg gcagcctcgg accggtgctc cctgtggtca cctccgcctc
     1981 caaactgtcg ccgcctctgc tctcctcagt ggcctccctg tcggccttcc ccttctcttt
     2041 cggctccttc cacttactgt ctcccaatgc actgagccct tcagcaccca cgcaggctgc
     2101 aaaccttggc aagccctata gaccttgggg gacggagatc ggagcttttt aagacccagc
     2161 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     2221 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2281 atcttgtgga aaggacgacc atctacgcct gccgggcggg cgacgcgtta agtcgacaat
     2341 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2401 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2461 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2521 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2581 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2641 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2701 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2761 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2821 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2881 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     2941 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3001 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3241 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3301 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3421 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3481 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3601 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     3661 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3721 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3781 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     3841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4021 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4081 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4141 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4201 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4261 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4321 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4381 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4441 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4501 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4561 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4621 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4681 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4741 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4801 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4861 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4921 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4981 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5041 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5101 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5161 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5221 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6301 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6361 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6421 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6481 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     6541 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     6601 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     6661 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     6721 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6781 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6841 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     6901 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     6961 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7021 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7081 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7141 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7201 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7261 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7321 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7381 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7441 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7501 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     7561 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     7621 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     7681 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     7741 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     7801 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     7861 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     7921 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     7981 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8041 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8101 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8161 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8221 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8461 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     8521 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8581 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     8641 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     8701 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     8761 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     8821 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     8881 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     8941 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9001 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9061 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9121 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9181 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9241 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9301 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9361 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
