LOCUS       sequence-347055-       11210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pdpy-30 DAF-2 unc-54 3' UTR C.elegans pan-tissue expression of DAF-2
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH4725
FEATURES             Location/Qualifiers
     source          1..11210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8338..8355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8338..8354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8347..8369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8567..9022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8654..8673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8864..8885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9039..9058)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9158..9180
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9218..9236)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9304..9408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9409..9477,9478..10269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9627..9646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10440..11028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10929..10948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11182..11199
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacgcgg ccgcctagac acgcgtgtaa cgtacaacat aaatgtttga
      301 cgtcaaccag atgtagtaca ataaaaaata tattcagatt acctagaaaa ttacttcggt
      361 catgaccacc aattttttga tttttcgatt ttcgaaaccg acatcgttga agaacattca
      421 ggtcataatt ttttatttga aaaataatgt tttctatgta ttaattcggt ttagtcgatc
      481 gaatatgtac agttgagaat tactagagta cggtgcgcaa gtatgcaaac ttgcgtaacg
      541 gtgtttacac atttgtcacg tttctctgcg tctcttcgtc tcaacaatgc ttgcactccg
      601 tcacattttc gaattgtcgg ccgatatcgg tatttagata aattcagtgc tctccttttt
      661 ttctccattt tactttcatc aatctttttt aaaaatcaat attcagcaaa ctattgaaaa
      721 attaattcaa aaaacacgac tggtcggaag ttatttgcat aatcaataga aatttcgttt
      781 tccgctcgct gtcagtgttt tgtcctgttt gtatttccca gtgcactttt cgagtggctc
      841 aagtttttat ctattttcct gcaaacttgc atatattgtt ttcttccaga agcctttgat
      901 atctcaataa ttttatactt gtttcaggga tcccccggga tgaatattgt cagatgtcgg
      961 agacgacaca aaattttgga aaatttggaa gaagagaatc tcggcccgag ctgctcgtcg
     1021 acgacttcaa caaccgctgc caccgaagct ctcggaacaa ccactgagga tatgaggctt
     1081 aagcagcagc gaagctcgtc gcgtgccacg gagcacgata ttgtcgacgg caatcaccac
     1141 gacgacgagc acatcacaat gagacggctt cgacttgtca aaaattcgcg gacgcggcgt
     1201 agaacgacgc ccgattcaag tatggactgc tatgaggaaa acccgccatc acaaaaaact
     1261 tcaataaatt attcttggat ttctaaaaag tcatcaatga cgtcattaat gcttttactg
     1321 ctattcgctt ttgtacagcc gtgtgcctca atagtcgaaa aacgatgcgg cccaatcgat
     1381 attcgaaata ggccgtggga tattaagccg caatggtcga aacttggtga tccgaacgaa
     1441 aaagatttgg ctggtcagag aatggtcaac tgcacagtgg tggaaggttc gctgacaatc
     1501 tcatttgtac tgaaacacaa gacaaaagca caagaagaaa tgcatcgaag tctacagcca
     1561 agatattccc aagacgaatt tatcactttt ccgcatctac gtgaaattac tggaactctg
     1621 ctcgtttttg agactgaagg attagtggat ttgcgtaaaa ttttcccaaa tcttcgtgta
     1681 attggaggcc gttcgctgat tcaacactat gcgctgataa tttatcgaaa tccggatttg
     1741 gagatcggtc ttgacaagct ttccgtaatt cgaaatggtg gtgtacggat aatcgataat
     1801 cgaaaactgt gctacacgaa aacgattgat tggaaacatt tgatcacttc ttccatcaac
     1861 gatgttgtcg ttgataatgc tgccgagtac gctgtcactg agactggatt gatgtgccca
     1921 cgtggagctt gcgaagagga taaaggcgaa tcaaagtgtc attatttgga ggaaaagaat
     1981 caggaacaag gtgtcgaaag agttcagagt tgttggtcga acaccacttg ccaaaagtct
     2041 tgtgcttatg atcgtcttct tccaacgaaa gaaatcggac cgggatgtga tgcgaacggc
     2101 gatcgatgtc acgatcaatg cgtgggcggt tgtgagcgtg tgaatgatgc gacagcatgc
     2161 cacgcgtgca agaatgtcta tcacaaggga aagtgtatcg aaaagtgtga tgctcacctg
     2221 taccttctcc ttcaacgtcg ttgtgtgacc cgtgagcagt gtctgcagct gaatccggtg
     2281 ctctcgaaca aaacagtgcc tatcaaggcg acggcaggcc tttgctcgga taaatgtccc
     2341 gatggttatc aaatcaaccc ggatgatcat cgagaatgcc gaaaatgcgt tggcaagtgt
     2401 gagattgtgt gcgagatcaa tcacgtcatt gatacgtttc cgaaggcaca ggcgatcagg
     2461 ctatgcaata ttattgacgg aaatctgacg atcgagattc gcggaaaaca ggattcggga
     2521 atggcgtccg agttgaagga tatatttgcg aacattcaca cgatcaccgg ctacctgttg
     2581 gtacgtcaat cgtcaccgtt tatctcgttg aacatgttcc ggaatttacg acgtattgag
     2641 gcaaagtcac tgttcagaaa tctatatgct atcacagttt ttgaaaatcc gaatttaaaa
     2701 aagctattcg attcaacgac ggatttgacg cttgatcgtg gaactgtgtc aattgccaat
     2761 aacaagatgt tatgcttcaa gtatatcaag cagctaatgt caaagttaaa tataccactc
     2821 gatccgatag atcaatcaga agggacaaat ggtgagaagg caatctgtga ggatatggca
     2881 atcaacgtga gcatcacagc ggtcaacgcg gactcggtct tctttagttg gccctcattc
     2941 aacattaccg atatagatca gcgaaagttt ctcggctacg agctcttctt caaagaagtc
     3001 ccacgaatcg atgagaacat gacgatcgaa gaggatcgaa gtgcgtgtgt cgattcgtgg
     3061 cagagtgtct tcaaacagta ctacgagacg tcgaacggtg aaccgacccc ggacattttt
     3121 atggatattg gaccgcgcga gcgaattcgg ccgaatacgc tctacgcgta ctatgtggcg
     3181 acgcagatgg tgttgcatgc cggtgcgaag aacggtgtat cgaagattgg ttttgtgagg
     3241 acgagctact atacgcctga tcctccgacg ttggcactag cgcaagtcga ttcggacgct
     3301 attcatatta cgtgggaagc gccgctccaa ccgaacggag acctcacgca ttacacaatt
     3361 atgtggcgtg agaatgaagt gagcccgtac gaggaagccg aaaagttttg tacagatgca
     3421 agcacccccg caaatcgaca acacacgaaa gatccgaaag agacgattgt agccgataag
     3481 ccagtcgata ttccgtcatc acgtaccgta gctccgacac ttttgactat gatgggtcac
     3541 gaagatcagc agaaaacgtg cgctgcaacg cccggttgtt gttcgtgttc ggctatcgga
     3601 gaatcatcgg aacagaacaa gaagaagcga ccggatccga tgtcggcgat cgaatcatct
     3661 gcatttgaga ataagctgtt ggatgaggtt ttaatgccga gagacacgat gcgagtgaga
     3721 cgatcaattg aagacgcgaa tcgagtcagt gaagagttgg aaaaagctga aaatttggga
     3781 aaagctccaa aaactctcgg tggaaagaag ccgctgatcc atatttcgaa gaagaagccg
     3841 tcgagcagca gcaccacatc cacaccggct ccaacgatcg catcaatgta tgccttaaca
     3901 aggaaaccga ctacggtgcc gggaacaagg attcggctct acgagatcta cgaaccttta
     3961 cccggaagct gggcgattaa tgtatcagct ctggcattgg ataatagtta tgtgatacga
     4021 aatttgaagc attacacact ttatgcgatt tctctatccg cgtgccaaaa catgacagta
     4081 cccggagcat cttgctcaat atcccatcgt gcgggagcat tgaaacgaac aaaacacatc
     4141 acagacattg ataaagtgtt gaatgaaaca attgaatgga gatttatgaa taatagtcaa
     4201 caagtcaacg tgacgtggga tccaccgact gaagtgaatg gtggaatatt cggttatgtt
     4261 gtaaagctta agtcaaaagt cgatggatca attgttatga cgagatgtgt cggtgcgaag
     4321 agaggatatt caacacggaa tcagggtgtc ctattccaga atttggccga tggacgttat
     4381 tttgtctcag taacggcgac ctctgtacac ggcgctggac cggaagccga atcctccgac
     4441 ccaatcgtcg tcatgacgcc aggcttcttc actgtggaaa tcattctcgg catgcttctc
     4501 gtctttttga ttttaatgtc aattgccggt tgtataatct actactacat tcaagtacgc
     4561 tacggcaaaa aagtgaaagc tctatctgac tttatgcaat tgaatcccga atattgtgtg
     4621 gacaataagt acaatgcaga cgattgggag ctacggcagg atgatgttgt gctcggacaa
     4681 cagtgtggag agggatcatt cggaaaagtg tacctcggaa ctggaaataa tgttgtttct
     4741 ctgatgggtg atcgtttcgg accgtgtgct attaagatta atgtagatga tccagcgtcg
     4801 actgagaatc tcaactatct catggaagct aatattatga agaactttaa gactaacttt
     4861 atcgtcaaac tgtacggagt tatctctact gtacaaccag cgatggttgt gatggaaatg
     4921 atggatcttg gaaatctccg tgactatctc cgatcgaaac gcgaagacga agtgttcaat
     4981 gagacggact gcaacttttt cgacataatc ccgagggata aattccatga gtgggccgca
     5041 cagatttgtg atggtatggc gtacctggag tcgctcaagt tttgccatcg agatctcgcc
     5101 gcacgtaatt gcatgataaa tcgggatgag actgtcaaga ttggagattt cggaatggct
     5161 cgtgatctat tctatcatga ctattataag ccatcgggca agcgtatgat gcctgttcga
     5221 tggatgtcac ccgagtcgtt gaaagacgga aagtttgact cgaaatctga tgtttggagc
     5281 ttcggagttg ttctctatga aatggttaca ctcggtgctc agccatatat tggtttgagt
     5341 aatgatgagg tgttgaatta tattggaatg gcccggaagg ttatcaagaa gcccgaatgt
     5401 tgtgaaaact attggtataa ggtgatgaaa atgtgctgga gatactcacc tcgggatcgt
     5461 ccgacgttcc tccagctcgt tcatcttcta gcagctgaag cttcaccaga attccgagat
     5521 ttatcatttg tcctaaccga taatcaaatg atccttgacg attcagaagc actggatctt
     5581 gatgatattg atgatactga tatgaatgat caggttgtcg aggtggcacc ggatgttgag
     5641 aacgtcgagg ttcagagtga ttcggaacgt cggaatacgg attcaatacc gttgaaacag
     5701 tttaagacga tccctccgat caatgcgacg acgagtcatt cgacaatatc gattgatgag
     5761 acaccgatga aagcgaagca gcgagaagga tcgctggatg aggagtacgc attgatgaat
     5821 catagtggag gtccgagtga tgcggaagtt cggacgtatg ctggtgatgg agattatgtg
     5881 gagagagatg ttcgagagaa tgatgtgcca acgcgacgaa atactggtgc atcaacatca
     5941 agttacacag gtggtggtcc atattgccta acaaatcgtg gtggttcaaa tgaacgagga
     6001 gccggtttcg gtgaagcagt acgattaact gatggtgttg gaagtggaca tttaaatgat
     6061 gatgattatg ttgaaaaaga gatatcatcc atggatacgc gccggagcac gggcgcctcg
     6121 agctcttcct acggtgttcc acagacgaat tggagtggaa atcgtggtgc cacgtattat
     6181 acgagtaaag ctcaacaggc agcaactgca gcagcagcag cagcagcagc tctccaacag
     6241 caacaaaatg gtggtcgagg cgatcgatta actcaactac ccggaactgg acatttacaa
     6301 tcgacacgtg gtggacaaga tggagattat attgaaactg aaccgaaaaa ttatagaaat
     6361 aatggatctc catcggtact ggattttttt ttttggagaa attagccgga aaattcgatt
     6421 tttttcgctg aaaatctgaa aaaatttggc taaaaattgc cctgaaacac gtttttttcg
     6481 gaattttcat ataaaaatgg acattttcgt aaaaaattgg ctatgtgttg tgaatttttt
     6541 ctgactttta aaggcctttg aactaggaat attaaaattt tattgaaaat tattgaaaag
     6601 tgcaaatttt tatttttttt aggtgaaaaa agcttagatt taggcgaatt ttctgaaatt
     6661 ttatagtgaa tttggttctc aggatattag ccattttttc ccgatttttt ggtaaaaatt
     6721 tgttaaaacc cgcttcaaat gactgtagtt ttatacaaaa ataactaaaa attcgatttt
     6781 tcaattattc agctcgaaaa agcccaacaa aaatatttaa aatttgaaga aaaaagctaa
     6841 aaatgagtaa aaatgactgg tttttttcgc tttttctatt tgaataaatt tctgaaaaat
     6901 gacgaaaatc tgaaaaaatt accccggaca ttgggaaatt tcgaaaaaat cttgaaaata
     6961 gtctgaaatt tttaaaattt acagattttc acaaaaaagg gaaatttcgc atttcctgaa
     7021 attgcccaaa aatttttttt ttcatttttt taaaaattct ggaaatttat ttttggctgt
     7081 ttcaaatttt taaaaaaaga aaaatcccaa aaattggtga aaactccatt aaaattctcc
     7141 agaaaattca aattttccat gaaaaattgt tttaaaagca tgaaaatctg ccgatttttg
     7201 tctaaaattt ttaaaaatat ttcaaaaatt tcagaaaaaa caggaaaagc gaatattaca
     7261 taaataaaaa atctggaaat tctagaaact gaaaattttt taatttttcc gctcaaaaat
     7321 tctaatattc gatgaaaatg agcccgaaaa ttcgagtaaa tttccaaaaa aaattttttt
     7381 ttttttcaaa cacaaatatt aatttttatt aatttttcag cgaaacggca acagccgtga
     7441 cattttcaac ggacgttcgg ctttcggtga aaatgagcat ctaatcgagg ataatgagca
     7501 tccacttgtg gtaccatggt attgatatct gagctccgca tcggccgctg tcatcagatc
     7561 gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     7621 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaaac ttcttcttaa
     7681 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     7741 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     7801 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttttacttc
     7861 tgataaattt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     7921 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     7981 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     8041 agtttatgaa attaattttc ctgcttttgc tttttggggg tttcccctat tgtttgtcaa
     8101 gagtttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     8161 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     8221 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     8281 accaaacata actgtttcct actagtggcc acgtgggccg tgcaccttaa gcttggcact
     8341 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8401 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     8461 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     8521 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     8581 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8641 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8701 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8761 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     8821 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8881 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     8941 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     9001 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     9061 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9121 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9181 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     9241 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     9301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9661 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9721 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9781 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9841 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9901 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9961 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    10021 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    10081 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10141 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10201 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10261 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10321 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10561 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    11041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    11161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
