LOCUS       sequence-347191-       15959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the CjcCas7-11 full locus for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 15959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0044 CjcCas7-11 full locus with synthetic a...
FEATURES             Location/Qualifiers
     source          1..15959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     539..558
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(958..963,964..980,981..986))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1098..1643
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1544..1563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1760..1777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1892..1926
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     promoter        2062..2166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2167..2961
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2167..2961
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2221..2240)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2831..2850
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(15679..15959,1..910))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca
       61 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
      121 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
      181 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
      241 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
      301 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg
      361 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
      421 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
      481 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
      541 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
      601 tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
      661 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
      721 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
      781 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
      841 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
      901 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
      961 aaagcttatc gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg
     1021 acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata ttttatctga
     1081 ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
     1141 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac
     1201 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
     1261 ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg
     1321 tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1381 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     1441 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     1501 accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc
     1561 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     1621 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag
     1681 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg
     1741 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca
     1801 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg
     1861 acaccctcat cagtgccaac atagtaagcc attgacagct agctcagtcc taggtataat
     1921 gctagcgccg atttaggcaa aaacggcttg aagactaaag gaaggaatta atgtcacggt
     1981 acaaccgcca atataacaat acaggtatta tagttgcttg aagactaaag gaaggaatta
     2041 atgtcacggt acgtatacgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2161 aagagtatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     2221 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     2281 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     2341 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     2401 gctgtgctcg acgttgttac tgaagcggga agggactggc tgctattggg cgaagtgccg
     2461 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     2521 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     2581 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     2641 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     2701 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     2761 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     2821 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     2881 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     2941 cttcttgacg agttcttctg aactgtcaga ccaagtttac tcatatatac tttagattga
     3001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacgtgc ccttaaacgc
     3121 ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa
     3181 attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg
     3241 gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg
     3301 acaacatcct ttccctgaga atttgatttt ttcaaattta ctccttaata agtcgtgcaa
     3361 tttcttttag gagtgacaat gttttttata ttcttgctta ttcccgaatt ctgtttgaga
     3421 tttgtttttt tctgaatgca tcttactttg gtactagtgt tacttattaa gtcgttttat
     3481 tacgatggaa gtgatataaa ttactggttt agtctataag ctaaaaaact attgacagat
     3541 aaaaataaaa tgaatatcat gctacctcta atcctaatct tcaaattcca aatactatca
     3601 atacctgcca aatgaaaaat agagtacaga ttgaagcaat cataagaaat cttcaaggcg
     3661 ctgcaagaga ctctaagacg aacaaactat cagagaacat cattgcttat gacgaataca
     3721 ggaagatcca taagagcgct tctttgtacc aatttggcat aatccccgcg aaagaatcat
     3781 catcggtgct tgcagagaat gaaactaatc atgtcgcttg tgaaaacgct atttttgaaa
     3841 tggcagaaaa gaatatagag aatttttcct ccgaagatat acataagaaa cgcaaagaaa
     3901 cgattgaatc tgccttgaga ctacttatgg gtctttataa ggatagacat gaaaaacttc
     3961 agccgagaac ctttgtcctc atcgcaaagg cataccttct gagaagcctt attactcgtc
     4021 caaaaggtat aacgataccc gaaaagaaaa aagaggcgct gaaaaaaggg attggctttg
     4081 ttgaaagtgc cattaaaaaa atccaatcct ccgaaaatat tttatctcat tcttctgata
     4141 tagatttgct tgagaaggca tggaggatca aaagtcagtt gtatcttgag tattatcggg
     4201 ttaacaagga tgaatgtgac aaaaatacat taaaagaagt tctcgaaaat tccctaatat
     4261 cgggatgtga taaatttgac aaaaatatcg aagacgtaca gattgctatc cgctactgtg
     4321 aattagagag tagtagagaa tatttggaac aaattatttc ctctcacctg gaaggtatag
     4381 aatttgagaa ggccagggca tacaaactcc ttgaacttga aaatgaaaat gaagatgaaa
     4441 taagaaaaag catgaaggtt gttattgaag agtatttatc gggtttttct gacccattat
     4501 gggaagatgc agttgagttt atcaataaac ttaaatccga caataagaat tgctggaagg
     4561 aactatcgtt agatatgtat aaggtttgcc gagaacaaga ggcggaaact gcgtctctcc
     4621 atttgcgttg gtactggtca agacagagaa ggctttacga cctggcattc attgcagcag
     4681 ataaagaaga agaaaaggca aaaattgccg attcattgaa aagtcgcctc tcgcttcgtt
     4741 ggtcagcatt agaagagacg ggtaaaaaat caaaaaataa acgggaaaaa gaagaaataa
     4801 gcagaatcct tgaagccgaa gcagtagcga tgcttggagg atatatcaag ggtgcacgga
     4861 agatcttaaa aaagaggaga agacctttac ctgatgagca acgttccata ccaaaagact
     4921 ggatagtcat ccatttttat gtaaatcaat tagaaaacaa gtgctatgcc cttatttata
     4981 acaaggatga aaatacctgg aagtgtgaat ttgttaaaga ataccaaaga ttgtttcatg
     5041 tctttttgac ttggcagaca aactataacc gatgtaaaga gagggctgcg gattcccttg
     5101 tgcaactctg caaagagatt gggaatgcca tgccctttct ctttgatgaa tgtattattc
     5161 cacaagataa aaatgtttta ttcattcccc acgatttcct gcatcgattg ccgcttcatg
     5221 gggcaataca tgaaaaaaac aacggtgtat ttttagaaaa tcatccatgc tgttaccttc
     5281 ctgcgtggtc atttgctgcg aaagaaaata atgcggtagt acagggaagc atcttgctca
     5341 agaatttccc tgaatattca tatgaggagt tagtttccaa ttcaacacta tggacttctc
     5401 cggtaaaaga cccggcgagt cctgatgacc tcaaaacaat tatcgcttca ccggaaatgc
     5461 ttgttattct ttgtcatgga gaagcagatg ctgtaaaccc tttcaatgcc aggcttaagt
     5521 taacaggaaa cggcatatcg catctcgaga tattacaaag tacaaagatg attttaaaag
     5581 gcagtaaaat aatcctgggc gcttgtgaaa cagacctcgt accgccacta tcagatatta
     5641 tggatgaaca tctgtctatt actacagcat ttcttacaaa cgacgccagg gagattttgg
     5701 gaacgatgta cgaggcactt gatgtacgca tatcaagcat cattcaaaag atatataggc
     5761 aagaacatta tagtagtatg atgaagcaat tgtgggaatg gcaaaaagtt ggggtagaaa
     5821 attatcgtga aaatggtgat acaccagcat tttataacac cgttgttttt cgtgttattg
     5881 gattatcaat atgagtaaaa atgcagaaga aatacttcat tcctgcctcg aaatgagagg
     5941 gtctggaaaa ctttgtgttg aatgctcagt tctcaaaaaa ggaacgtgtc aaatattgat
     6001 gcatgaaata ctgaaagtca aagatgcatt aaaattaccc aaagatgata tccgggatat
     6061 aatctcagaa tcaattgaag ctattcagct tggcaacttt aaaggaacca cccatatcca
     6121 gtttagggta tgggtacgaa gaataataca aaacaagaag aatgattatt ttagaaataa
     6181 atataaaaga aaagaacatg agaatgcact ccccattcca atggatgatg agaaacacga
     6241 gattatctta tttgataaac cagatgcaac tcaggcagac gaatttaaac gcattgaaaa
     6301 tgagcatgat gtggaaaaaa ttttacagag tttaaggaaa aaggtaattg agggtgaaat
     6361 tgaaaaaaat gatcttgatt tagtacaagc ctgttatgat ggttttaagg aaggcttatc
     6421 tcaggaagaa atagcaaaga gcctatcttt aaaaccaaat actctcaatc aaaacttaag
     6481 ccgcctgaga aaaaaattga aggctgaaag agaggaatat tgcaggaatg attaacatga
     6541 cgaacatacc aataggccat gtttttccag aagctttagt aatagacaga gatatttata
     6601 ataaacgatg gatacttaaa gtggttacag ataacaatga aaaaaaagat actgtaaagc
     6661 taaagtcgaa aataagtggc tttaatgaaa ttttacaaga aaccaaactt atcttggctg
     6721 ataagtatgt accacctggc gtagaaatta aagaaacaga aatagtaaaa attcttgtag
     6781 taataaaaaa agaacagaac aaagcatacg ttgcactaaa aggatacagt aaaatcctga
     6841 gagatcattt ctattttaaa tatctgctta acgaatccat taaaacccta cttggcattc
     6901 ctattgaagc tcctctttct agaagtaaag ttatttataa accaaaaaaa tccaattgga
     6961 aattgtggtc taataaattg agagaggatt gtggagaaca gtttaaaaac aacttcaaag
     7021 tggaagatga agtttacaat gttgatatcc catttttaaa tgtatatata gtaaggggaa
     7081 aagattacgt ttttcctgta tttggtttta aatatctttc cacgttaatg gggaaactat
     7141 tattttatcc tgatattatc gatacaaatg gtaattctga gaggagtgtt tatagaacag
     7201 atggtttgaa taatttcccc atgcaattca cagctaaaat taaaaatagt ggtgccctca
     7261 aagatgttcg tgtttttttg cacggtttta cgtatccagc aaagtggcac tttggcatat
     7321 ggaagcatat gttttcctta ttgccaggcg cgaacgaaga tgaaaccgca ttatgggaaa
     7381 aaatgttgag gtatagcttc tttccagaga aaacttttaa attttgcaaa ccaaatcagg
     7441 ataacagctc ctgtgatgaa gaacattgtg tttcattatc gttatttaaa gatatacacc
     7501 cccagataaa agattattgc aggttgtatt tagaacaatt tgtcacttta cttaagtcaa
     7561 atacgattaa taatgtagca atatttaaag acagacactc ctgtaataaa atcgaattga
     7621 tttgtgtttg tcaaaataag ggtatctacc agaaggcgat atttcgtgga gtatgttata
     7681 attgggatag taataattcc cattttgtat ttcatttgac gcctgttact atgtacttag
     7741 ataaaaataa taaattattt catatgcgca caaaggataa atctattttt tctggaagtt
     7801 tgtgtgagat aaagcaatgc aattaacaga caaaagtaga aacgaattgt ttagcgctct
     7861 ccttgaatgg ggtaagtcac acatgctttc gccagaaatt gttcaagata tctctgagat
     7921 cgaggagaac tgcgagcctt ttataaaaag tgatgaattt acaaactttt tattaaattc
     7981 agtggaaaaa atcaggcggc aagtagaatt tgcaatttcc ttgtttacca atatagagct
     8041 agttactgca actgatgaag attctctatg gcatgatgca gaggtgtctt ttattgcctt
     8101 cgatcgagta aactatttga tggaggtatt acagttttta attgtacccc gcaaaaagga
     8161 aattgctgaa aaagctatga aggcgttaga taccatattt gaaaaaacaa taggctggct
     8221 tgaagaaagt gagttttcac cattaaggtt agtagtactt aatgaatccc gccgttatca
     8281 tcttgaacaa ataccagaag atgaacgata caggttccct tggtatgaat tatattcgga
     8341 ttatagtgaa aataccctgg aaataattat tgaaaacttc gacacttttt tgtcgggaaa
     8401 atgggaaaaa ttaacgaggg agatacccca agagtacctg catgaaatat cgcttgaatt
     8461 aaaaagagat aaactattac tttctcgaat aaaacaagag gcatcttatc ataaaagatt
     8521 actcgctgcg gtatcaaaac cctcatctct taagttatgg cgattgggag atgaagcggc
     8581 attggattac tttcttcctg gtggggtaaa aaaatctggt gcagtgcgag ttagtttaaa
     8641 attgatagaa gatgcaaaga ttgcttttga ggcagatgag atttgttgga aattccttgc
     8701 tgctttttgt gggccaaatt tggatgataa acaacgcctc tccctgcttg ataaagtgga
     8761 ggaaaggata aagaaaattg atatacataa aatttcagaa aatgaaaata tcctgaaaac
     8821 actaaaatct tggtttgagg gaaattgcga taatgcaaaa cttattaaga tttcttttga
     8881 cacttggatt aaaatgatgg aagaaaaagc aatcaacatg catgctgtag agtttgacga
     8941 atcgccagaa aagttgtgga atgccataat ggaacttcaa aaagggagaa tgggcgcttc
     9001 aaattctgtt gaaaaatata taaacgattt tgttaatggg tgccgacaag tatggcaaat
     9061 tttcaaatcg accgttgatg aaccatcggt attgtatggt gctcgggcta ttgctgcatc
     9121 atctgaaaag tctgtgcaac caagaaagtt agaactcaat aataatccaa ttcttctttc
     9181 gttgaagccg aaccctaaag gagaatatat tattttatca tcgctggcat taaaaagggt
     9241 agttcttggt gaaggtgttg aggattatga gaagatatgg aactatcttg agcataccaa
     9301 aaatgattac tggtgcggct gttttattac aaatgatgac aaacctgatg tagcttctgt
     9361 acaacaaata gagaatcgaa ttttggcaaa aaaaacgaga gattataaaa aagcaattat
     9421 tggtgtatct cctgaaaaaa tcgttttaga agaattcatt caggaattgc cggctgtgat
     9481 atttgaagga aaaggcccgt taaaggattc tttagttaag aaggttatca tattggttat
     9541 ttcacttgaa tgaggtgtta tcttgcatac aatattgcct atccatctta ccttcctcga
     9601 accctaccgc cttgctgagt ggcatgcaaa agctgataga aagaaaaata agagatactt
     9661 aagaggcatg agctttgccc agtggcataa ggataaggat ggcataggga aaccttatat
     9721 cacaggtacg cttttgaggt cggcagtcct aaatgcagcg gaagagctta ttagtctgaa
     9781 tcagggaatg tgggctaaag aaccttgctg caacggcaag tttgagacag agaaagataa
     9841 gccggctgtc ctcagaaaga ggccaacaat ccagtggaaa accggaagac cggcaatatg
     9901 tgaccctgaa aaacaggaga agaaagacgc atgtcccctc tgtatgctcc tgggcagatt
     9961 tgataaggcg ggcaagagac acagggataa taaatacgat aaacatgatt atgacatcca
    10021 cttcgataac ctgaacctta taaccgacaa aaaattctcc catcctgatg atattgcatc
    10081 ggagcgaatc ctcaaccgtg ttgattacac cactggcaag gcgcatgatt attttaaggt
    10141 atgggaggtg gatgatgatc aatggtggca atttaccggt acaataacga tgcatgatga
    10201 ttgctcaaag gcgaaagggt tgctcctggc ttctctgtgt tttgttgaca agctctgtgg
    10261 ggcgctttgc aggattgaag tgactggaaa taatagccag gatgagaata aagagtatgc
    10321 gcatccagat accggtatta ttacatccct aaatttaaaa taccagaaca attccacaat
    10381 tcatcaagat gccgtaccat tatccggctc cgctcatgat aacgatgaac ctcctgttca
    10441 tgacaatgac agttccctgg ataacgatac gattacgctc ctgtcaatga aagctaaaga
    10501 aattgtcggg gcttttcgtg aatccggcaa gatagagaag gcaaggacac tggcagatgt
    10561 gataagggct atgcggctac agaaaccgga tatttgggaa aaattgccaa aaggcataaa
    10621 tgataaacac cacctgtggg atagagaagt gaacggcaag aagttaagga atatattaga
    10681 ggaattatgg cggctcatga acaagaggaa cgcatggagg acattctgtg aagttctggg
    10741 gaatgaactc taccggtgtt ataaggaaaa gacgggtgga attgtgttac ggttcaggac
    10801 acttggcgag acggagtact atccggaacc ggagaaaaca gagccatgcc taatctcaga
    10861 taattctata ccaattacac cacttggagg tgttaaagaa tggattatta tcgggagatt
    10921 aaaagcggag acgccgtttt attttggtgt gcagtcatcc tttgattcta cacaggatga
    10981 cctggacttg gttcctgata ttgtcaatac tgatgaaaaa cttgaagcaa atgaacaaac
    11041 cagcttcagg atactgatgg ataagaaagg gcgttaccgt atccctcgct cactcataag
    11101 aggcgtcctc cgcagagatt tgagaacggc atttggcggg agtggttgca ttgtggaatt
    11161 aggcagaatg attccatgcg attgtaaggt ctgtgctatt atgcgaaaaa ttactgtaat
    11221 ggattccagg agtgagaata tcgaattacc cgatattcgt tatagaatca ggctaaaccc
    11281 ttacaccgct acggtagacg agggggcgtt gtttgatatg gagataggac cggaagggat
    11341 tacttttccg tttgttttcc gttatcgtgg ggaagatgcg ctaccgcgtg aattgtggag
    11401 cgtaattcgc tattggatgg atggcatggc ctggcttggt ggttccggat ctacgggtaa
    11461 aggacgtttt gctcttatcg atataaaagt gtttgaatgg gatttgtgta atgaagaagg
    11521 gcttaaagca tacatttgtt caagaggatt aagaggcatc gaaaaagaag tattgcttga
    11581 gaataaaacg atagcagaaa ttactaattt atttaaaaca gaagaagtca aattctttga
    11641 atcatacagt aaacatatta aacaattgtg tcacgaatgc attataaacc aaatatcctt
    11701 tttgtgggga cttaggtctt attatgaata tctcggtccc ctgtggacag aggtcaaata
    11761 cgaaatcaag attgcctcac ctttattatc ctccgatacc atatcggcct tgctgaacaa
    11821 ggataatatt gattgtatag catatgagaa gaggaaatgg gaaaacggtg gaataaaatt
    11881 tgttccaacc atcaagggcg agactatacg gggtattgtg aggatggccg ttggaaagag
    11941 aagcggtgat ctgggaatgg atgatcatga agactgttcc tgtaccctgt gtacgatctt
    12001 tggcaatgag catgaggctg gcaagctgcg gtttgaggat ttggaagtcg tagaggaaaa
    12061 attaccgtct gaacagaatt ctgatagcaa taaaatacca ttcggcccag ttcaggatgg
    12121 cgatggtaac cgggagaagg agtgtgtgac tgcggtgaag agctataaga agaaactcat
    12181 tgaccacgtg gcaattgatc gtttccatgg cggtgcggaa gataagatga agtttaatac
    12241 cttgccgctt gcgggaagct ttgaaaagcc cattatcctg aaaggtagat tctggataaa
    12301 aaaggacatt gtaaaggact ataagaaaaa aatcgaagac gccatggttg atatacggga
    12361 cgggttgtat ccaataggcg ggaaaacagg cattgggtat gggtgggtta cagaccttac
    12421 catcctgaat ccccagagcg gatttcaaat tccggtaaaa aaagatattt ccccagaacc
    12481 aggaacgtat tctacttatc catcccacag taccccatcc cttaataaag gccatattta
    12541 ttacccgcac tatttcttag cgccggcaaa tacggtgcat cgtgaacaag agatgatcgg
    12601 ccatgagcag tttcacaaag aacagaaagg ggaattgctt gtttcaggca agattgtgtg
    12661 caccctgaag accgtaacac cccttatcat cccggataca gaaaatgagg acgcctttgg
    12721 tcttcaaaac acatattccg gacataaaaa ttaccagttt tttcatataa acgatgaaat
    12781 tatggtcccc ggctcagaga ttcggggtat gataagttct gtttatgagg cgattaccaa
    12841 ttcgtgtttt cgcgtttatg atgaaacgaa atatataaca cgccggttat cgcctgaaaa
    12901 aaaagatgaa tcaaacgaca agaataagtc tcaagatgat gcatctcaaa aaatacggaa
    12961 aggtttagtg aaaaaaaccg acgaagggtt ttcaatcatc gaagttgaaa gatattccat
    13021 gaaaaccaag ggcgggacaa agttagttga taaagtttac cgccttcctc tctatgatag
    13081 tgaagcagtt atagcatcta ttcaatttga gcagtatgga gaaaaaaatg aaaaaaggaa
    13141 tgcaaagatt cgggctgcaa taaaacggaa tgaagtaatt gcagaagttg cccgaaaaaa
    13201 tttgatattc ctcaggtcct taacgccgga agaattgaaa aaggttttac agggtgaaat
    13261 actggtaaaa tttagtctta agtcaggaaa aaatcccaat gattatttgg cggaattaca
    13321 tgaaaacgga accgagcgtg gattgataaa gtttaccggg cttaacatgg tgaatataaa
    13381 aaatgtaaat gaggaagata aagattttaa tgatacctgg gattgggaaa agctcaatat
    13441 cttccataat gcacacgaga agcgaaatag tcttaaacag ggatatccaa gacctgtctt
    13501 aaaatttata aaagatcggg ttgaatacac cattcccaag cggtgcgagc gtatattttg
    13561 tattccggta aaaaatacaa tcgaatacaa agtatcttca aaagtctgta aacaatacaa
    13621 agatgtactg agcgattatg agaaaaattt tgggcatatt aacaagatat ttaccacaaa
    13681 aatccaaaag agagaactta ccgacggtga tttggtctat tttattccca atgaaggcgc
    13741 agacaaaacc gtacaggcta tcatgcctgt gcctctttca cggataaccg acagcagaac
    13801 actgggagaa cgattacccc acaaaaacct cttgccttgc gttcacgagg ttaatgaagg
    13861 tcttttatcc ggcatattag attctcttga taaaaaactc ctttccatcc acccggaagg
    13921 cctttgccct acatgccggc tttttggtac gacatattac aaaggacggg ttcgtttcgg
    13981 gtttgcaaac cttatgaaca aacccaaatg gctgaccgag agggaaaatg gttgtggtgg
    14041 gtatgtgacc cttccactcc ttgaaagacc acgcctgaca tggtcagtac cctccgataa
    14101 atgtgacgta ccagggagaa aattttatat ccatcataat ggctggcagg aggtgctaag
    14161 aaacaacgat ataacaccaa agactgaaaa taatcgtacc gtagaaccat tagctgcgga
    14221 caatagattt acctttgatg tctattttga gaatctgagg gagtgggaat tagggcttct
    14281 ctgctattgc cttgaacttg agccgggaat ggggcataaa cttggcatgg gtaagccaat
    14341 gggctttggt agcgtaaaaa tagcaataga aaggttacag acctttactg ttcatcagga
    14401 tggtataaac tggaagccct ccgaaaatga aattggggtt tatgtgcaga aaggaagaga
    14461 aaagctggtt gagtggttta ccccttcggc tccgcacaaa aatatggaat ggaacggggt
    14521 aaaacacatc aaagacctcc gttctttact cagcataccc ggcgacaaac ccaccgtaaa
    14581 atatcccacc cttaacaagg acgctgaggg agcaatatct gactatacgt atgagcggct
    14641 ctctgataca aagctgctgc ctcatgataa acgggtagaa tacctgagaa ccccgtggag
    14701 tccatggaat gcctttgtga aggaagcaga gtattctcct tcagaaaaaa gtgatgaaaa
    14761 gggtcgtgaa actatacgca caaaacctaa atctctacct tctgtgaaat caattggtaa
    14821 ggttaaatgg tttgatgaag gtaagggttt cggtattctt attatggatg acggcaaaga
    14881 ggtatcgata agcaagaatt ctatccgggg aaatatcttg ctaaaaaaag gtcaaaaggt
    14941 gacgttccac attgtacagg ggttgatacc aaaggctgaa gatatagaaa tagcgaagta
    15001 aatacggtcc ggaacataat ggtgcagggc gcttgtttcg gcgtgggtat ggtggcaggc
    15061 cccgtggccg ggggactgtt gggcgctgcc ggcacctgtc ctacgagttg catgataaag
    15121 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctaccg
    15181 gcagcggtgc ggactgttgt aactcagaat aagaaatgag gccgctcatg gcgttgactc
    15241 tcagtcatag tatcgtggta tcaccggttg gttccactct ctgttgcggg caacttcagc
    15301 agcacgtagg ggacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
    15361 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
    15421 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
    15481 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg
    15541 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
    15601 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
    15661 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
    15721 ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct
    15781 cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt
    15841 aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
    15901 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatgggga
//
