LOCUS       sequence-347227-        5592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wrmScarlet11^SEC^3xMyc vector with ccdB sites for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Witten G, DeMott E, Huang G, Zelasko F, de Jesus B, Mulchand C, 
            Schuck L, Pullman S, Perez A, Mahableshwarkar P, Wu Z, Cardona EA, 
            Pierce JT, Dickinson DJ, Doonan R
  TITLE     mScarlet and split fluorophore mScarlet resources for plasmid-based 
            CRISPR/Cas9 knock-in in C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 14;2023:10.17912/micropub.biology.000871. 
            doi: 10.17912/micropub.biology.000871. eCollection 2023.
  PUBMED    37396790
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-14"; volume: 
            "2023:10.17912/micropub.biology.000871. doi"; pages: " 
            10.17912/micropub.biology.000871. eCollection 2023"
COMMENT     Sequence Label: pGLOW63
FEATURES             Location/Qualifiers
     source          1..5592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1..34)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(1888..2211)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    4129..4153
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4156..5181
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4156..5181
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5182..5206)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5559..5592)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ataacttcgt ataggatact ttatacgaag ttattttcag ggatacccat acgacgtccc
       61 agactacgcc taaggatgat cgacgccaac gtcgttgaat tttcaaattt taaatactga
      121 atatttgttt tttttcctat tatttattta ttctctttgt gttttttttc ttgctttcta
      181 aaaaattaat tcaatccaaa tctaaacatt tttttttctc tttccgtctc ccaattcgta
      241 ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca
      301 ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa
      361 taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa
      421 aacataaaaa tatatagagg aggaaggaaa actagctgcc ctcgattgtc tatcttccat
      481 tctcaccaag tttcaacaac tttattcagt cattcttctc tggttcattt cccaaccaat
      541 cttcccgaat aaagcccatc tgtcctttcg gccacattcc tcacctacat ccgtactcct
      601 tatctcccgt tcttatccag tcttttatcg tctctgcgtc tcgcacactg tctcgtcggt
      661 aatgtaaaaa aaaaacaaaa aaaacttgaa aacttgcgga aagatttcct aataaaagga
      721 gacgaatccg gtaggaaatc gacaagtctc tgcttctcga agactaggtg agttacattt
      781 ctttggccga attattttga aatcctaaat ttgataattt cagagatgtc tgtaaaactt
      841 gcgtgttatg tgacggcttc ggtcactgtc gccactctta tggtatgctt catgaccatg
      901 tcaaccatct actcggaggt tgatggattc agagagaagc ttgataccga gatgaatgtg
      961 ttcagagtga gttaaattat attttgaatt ttaaataatt tttaattttc tagcaatcta
     1021 ccaatggatt gtggaaggac atagttgtca tcggaagatc tagcaagcgt gtccgttgtc
     1081 aatatgaaga gaccaacgct accccaactc cacatgctga tggatcccca tctgctccac
     1141 caggtcaacc accagcagtt ccaccagtct tcaaccagcc aaagactcca aatggagcca
     1201 atggaaatgg accaacctgc aactgcaatg ctgataacaa gtgcccagct ggaccatccg
     1261 gaccaaaggg agttccagga gttccaggac tcgacggagt tccaggactt gacggtgttc
     1321 caggagttgg agctgatgat atcgctccac aacgcgagtc tgtcggatgc ttcacttgcc
     1381 cacaaggacc agttggacca ccaggagctc ttggaagacc aggaccacgt ggacttccag
     1441 gaccaagagg acaaaatgga aacccaggaa gagatggaca accaggacat ccaggagagc
     1501 aaggatcatc cggtcaaatc ggaaagatcg gagagccagg accaccagga gagaagggac
     1561 gcgacgccga gcatccaatc ggaagaccag gaccaaaggg accaagagga gatcaaggac
     1621 caacaggacc agctggacag aacggtcttc acggaccacc aggagagcca ggaaccgttg
     1681 gaccagaagg accatctgga aagcaaggac gtcaaggacc agacggaacc cagggagaga
     1741 ctggaccaga cggaagacca ggaaaggatg ccgagtactg ccagtgccca gacaagtctc
     1801 caccatcaga ggctgtcaac gccaaccgtg gatacagaaa tatctaaatt gttggtgttt
     1861 tctaataaaa tatttgagat tcagtgagac ttttttcttg gcggcacaat aaagtttcct
     1921 ctttttgttg ggtcactcaa cgaacacaat acaaatattt gagcgctcgc atttttctca
     1981 acctttcggg tttaaagcac ttcaaaatgg aattgaagag agattgtgaa agtacatcag
     2041 acagaatcac attttgatag cattcacttc actcagatgc aaacttttca gtgactataa
     2101 aaagtcataa aataataaca ttaaaaaagc aaataaatta gcgagagaat tttttgacaa
     2161 aaagaaagaa gagtgataga gaagaaggga atgcttgaaa ggattttgca tttatcttaa
     2221 tcaccgtctt cgagaagacg aaccatggcg ccagtttcag aatccaagtt tctaatatag
     2281 ttcatcacaa tgttgacgtt ggtccaacct ccggcttgca taatctctgg gatggaaact
     2341 ccagcgcgag ccatgtcacg ggcagctccg acacgggcgg aatgtccaga ccaagccaag
     2401 taacgttgtc cggaatcatc cttggctccg tagatgagac ggtgggtagc ttcgaagatt
     2461 cctgaaaatt tattttcagc ttattcttag ctaatatgtt gtttaaactt accttcaaga
     2521 gctcgagtcg agagttgtga tgttgcactc ggggcagcaa ctccgttctt gcgaacacga
     2581 cagaacaggt agttgtttgg atcatcggcg actccggaaa ccgaaatcca gcgctcaacg
     2641 agcttggtaa ctccaaggga gagcgctttt tcgactccgg cagtcgagac cagcgttttt
     2701 gtacgtccga tgtggatgag cattcttcct ccgtctgtgc gactaatatc cttgacgcga
     2761 atgcgggcaa tttcggcgat acggagaagt gtgttgtaag caattccgag gaaggcgagg
     2821 tttcggatgt cctggcaacg atcggaattt tccataagag aacggacctg atcgaagtcg
     2881 gttctgaaaa tttcgtcaag tcttaggatt gaatacatta atatttaaac ttacctttcg
     2941 aaggcgaggg cttgcttggc tctctctcca gcatcaacat tttccttgcg gatacgtctc
     3001 attacgagag atacggcatt agaatctgat gggcgtggaa gtccagatcg gcgatggagc
     3061 atattgagct gtccgagatg ttgttggatc gtcttcaccg ccaatccacg agcttggagg
     3121 tagagcaagt agtcacgaac gtcttctggc tcggctggga accacttgcg gttgttaagc
     3181 ttgcaccagg cagcccaaga acggcacact gagagaagca tcttccaggt gtgctcggag
     3241 aaggcttgtc tgtcacggaa catatccatg aggttcttgc gaacttcatc acttgtcgcg
     3301 tcaactggaa gtgctggaag attttgatga acagtgagaa gatttgacat attttcgaag
     3361 ttttttagat gcactagaac aaagcgtgtt ggcttcctct gagcccgctt tccttatata
     3421 cccgcattct gcagccttac agaatgttct agaaggtcct agatgcattc gtttgaaaat
     3481 actcccggtg ggtgcaaaga gacgcagacg gaaaatgtat ctgggtctct ttattgtgta
     3541 cactactttt ccatgtaccg aatgtgagtc gccctccttt tgcaacaagc agctcgaatg
     3601 ttctagaaaa aggtggaaaa tagtataaat accgttgaaa ataaataccg aacaacattt
     3661 gctctaattg tgaaattaga aatcttcaaa ctataatcat gtcactttac cactatttcc
     3721 gtccagctca acgttccgtt tttggtgatc atttttgctt tcgtcgtaaa tctacacacg
     3781 cgtctcttcc gtgcgagagt ccaagccagc agccaaattc gttgactgag tattcaacgt
     3841 ttatacgttg tcggcaacga gaaataggaa aatgcatcgg gaaatgttct tttttcgatt
     3901 ttttccaagg ttttgacaaa ttttaccacg aattttgcta tgttttcaat taaaaaatat
     3961 gttattcaac tgtttctatg aggaaaataa ggctttgcat gtaattttct tattcagcat
     4021 aatttttaat taatttgaat tttctgtcct aacgtttatt ttgttttctt ggttatgact
     4081 gatctgaaat taatttttga attttaaggt aatatgtcag gcggtgccgc aagtttgtac
     4141 aaaaaagcag gctccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4201 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     4261 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     4321 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     4381 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     4441 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     4501 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4561 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     4621 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     4681 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     4741 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     4801 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     4861 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     4921 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     4981 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5041 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5101 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5161 cgtccgaggg caaaggaata gacccagctt tcttgtacaa agtgggtcca attactcttc
     5221 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
     5281 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
     5341 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
     5401 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
     5461 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
     5521 atcatatgtt acgtttcagt ttatgaccgc aatttttaat aacttcgtat aggatacttt
     5581 atacgaagtt at
//
