LOCUS       sequence-347434-       11569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of MYOCD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-MYOCD
FEATURES             Location/Qualifiers
     source          1..11569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2950..2974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3053..3072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3053..3071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3103..3127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6095..6119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6145..6186
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6196..6213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6263..6851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6316..6336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6734..6745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6854..6870)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6855..6871)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7376..7556
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7582..7599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7588..7812
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7858..8286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7945..7964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8155..8176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8295..8315)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8300..8629
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8480..8615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8542..8561
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8677..8724
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8743..9117
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9247..9368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9284..9303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9338..9357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9417..9433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9417..9433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9430..9452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9441..9457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9465..9471,9472..9489,9490..9495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9510..9531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9648..9665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9819..10407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9899..9918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10578..11369,11370..11438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11201..11220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11439..11543)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     2941 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3001 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tgtaatacga
     3061 ctcactatag ggagacccaa gctggctagg taagcttgat caacaagttt gtacaaaaaa
     3121 gcaggcttca ccatgacact cctggggtct gagcattcct tgctgattag gagcaagttc
     3181 agatcagttt tacagttaag acttcaacaa agaaggaccc aggaacaact ggctaaccaa
     3241 ggcataatac caccactgaa acgtccagct gaattccatg agcaaagaaa acatttggat
     3301 agtgacaagg ctaaaaattc cctgaagcgc aaagccagaa acaggtgcaa cagtgccgac
     3361 ttggttaata tgcacatact ccaagcttcc actgcagaga ggtccattcc aactgctcag
     3421 atgaagctga aaagagcccg actcgccgat gatctcaatg aaaaaattgc tctacgacca
     3481 gggccactgg agctggtgga aaaaaacatt cttcctgtgg attctgctgt gaaagaggcc
     3541 ataaaaggta accaggtgag tttctccaaa tccacggatg cttttgcctt tgaagaggac
     3601 agcagcagcg atgggctttc tccggatcag actcgaagtg aagaccccca aaactcagcg
     3661 ggatccccgc cagacgctaa agcctcagat accccttcga caggttctct ggggacaaac
     3721 caggatcttg cttctggctc agaaaatgac agaaatgact cagcctcaca gcccagccac
     3781 cagtcagatg cggggaagca ggggcttggc ccccccagca cccccatagc cgtgcatgct
     3841 gctgtaaagt ccaaatcctt gggtgacagt aagaaccgcc acaaaaagcc caaggacccc
     3901 aagccaaagg tgaagaagct taaatatcac cagtacattc ccccagacca gaaggcagag
     3961 aagtcccctc cacctatgga ctcagcctac gctcggctgc tccagcaaca gcagctgttc
     4021 ctgcagctcc aaatcctcag ccagcagcag cagcagcagc aacaccggtt cagctaccta
     4081 gggatgcacc aagctcagct taaggaacca aatgaacaga tggtcagaaa tccaaactct
     4141 tcttcaacgc cactgagcaa tacccccttg tctcctgtca aaaacagttt ttctggacaa
     4201 actggtgtct cttctttcaa accaggccca ctcccaccta acctggatga tctgaaggtc
     4261 tctgaattaa gacaacagct tcgaattcgg ggcttgcctg tgtcaggcac caaaacggct
     4321 ctcatggacc ggcttcgacc cttccaggac tgctctggca acccagtgcc gaactttggg
     4381 gatataacga ctgtcacttt tcctgtcaca cccaacacgc tgcccaatta ccagtcttcc
     4441 tcttctacca gtgccctgtc caacggcttc taccactttg gcagcaccag ctccagcccc
     4501 ccgatctccc cagcctcctc tgacctgtca gtcgctgggt ccctgccgga caccttcaat
     4561 gatgcctccc cctccttcgg cctgcacccg tccccagtcc acgtgtgcac ggaggaaagt
     4621 ctcatgagca gcctgaatgg gggctctgtt ccttctgagc tggatgggct ggactccgag
     4681 aaggacaaga tgctggtgga gaagcagaag gtgatcaatg aactcacctg gaaactccag
     4741 caagagcaga ggcaggtgga ggagctgagg atgcagcttc agaagcagaa aaggaataac
     4801 tgttcagaga agaagccgct gcctttcctg gctgcctcca tcaagcagga agaggctgtc
     4861 tccagctgtc cttttgcatc ccaagtacct gtgaaaagac aaagcagcag ctcagagtgt
     4921 cacccaccgg cttgtgaagc tgctcaactc cagcctcttg gaaatgctca ttgtgtggag
     4981 tcctcagatc aaaccaatgt actttcttcc acatttctca gcccccagtg ttcccctcag
     5041 cattcaccgc tgggggctgt gaaaagccca cagcacatca gtttgccccc atcacccaac
     5101 aaccctcact ttctgccctc atcctccggg gcccagggag aagggcacag ggtctcctcg
     5161 cccatcagca gccaggtgtg cactgcacag aactcaggag cacacgatgg ccatcctcca
     5221 agcttctctc cccattcttc cagcctccac ccgcccttct ctggagccca agcagacagc
     5281 agtcatggtg ccgggggaaa cccttgtccc aaaagcccat gtgtacagca aaagatggct
     5341 ggtttacact cttctgataa ggtggggcca aagttttcaa ttccatcccc aactttttct
     5401 aagtcaagtt cagcaatttc agaggtaaca cagcctccat cctatgaaga tgccgtaaag
     5461 cagcaaatga cccggagtca gcagatggat gaactcctgg acgtgcttat tgaaagcgga
     5521 gaaatgccag cagacgctag agaggatcac tcatgtcttc aaaaagtccc aaagataccc
     5581 agatcttccc gaagtccaac tgctgtcctc accaagccct cggcttcctt tgaacaagcc
     5641 tcttcaggca gccagatccc ctttgatccc tatgccaccg acagtgatga gcatcttgaa
     5701 gtcttattaa attcccagag ccccctagga aagatgagtg atgtcaccct tctaaaaatt
     5761 gggagcgaag agcctcactt tgatgggata atggatggat tctctgggaa ggctgcagaa
     5821 gacctcttca atgcacatga gatcttgcca ggccccctct ctccaatgca gacacagttt
     5881 tcaccctctt ctgtggacag caatgggctg cagttaagct tcactgaatc tccctgggaa
     5941 accatggagt ggctggacct cactccgcca aattccacac caggctttag cgccctcacc
     6001 accagcagcc ccagcatctt caacatcgat ttcctggatg tcactgatct caatttgaat
     6061 tcttccatgg accttcactt gcagcagtgg tagaacccag ctttcttgta caaagtggtt
     6121 gatctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     6181 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctca
     6241 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6301 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6361 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6421 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6481 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6541 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6601 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6661 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6721 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6781 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6841 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     6901 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6961 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7021 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7081 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     7141 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7201 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7261 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7321 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7381 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7441 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7501 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7561 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7621 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7681 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7741 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7801 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     7861 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7921 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7981 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8041 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8101 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8161 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8221 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8281 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8341 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8401 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8461 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8521 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8581 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8641 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8701 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8761 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     8821 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     8881 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8941 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     9001 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     9061 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     9121 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9181 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9241 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9301 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9361 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9421 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9481 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9541 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9601 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9661 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9721 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
