LOCUS       sequence-347609-        9209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for GFP-TREX1 D272fs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pLENTI CMV GFP-TREX1 D272fs BLAST
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2311..2320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2317..2319,2320..2322,2323..3033)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2317..2319,2320..2322,2323..3033)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2362..2383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2623..2642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2970..2991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3043..3067
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3929..4517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3982..4002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4400..4411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4568..4588)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4573..4902
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4753..4888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4950..4997
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5016..5414
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5501..5734
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5806..5927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5843..5862)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5897..5916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5967..6102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6122..6141)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6123..6141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6151..6168)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6151..6167)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6160..6182)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6309..6764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6396..6415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6606..6627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6790..6894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6895..6963,6964..7755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7113..7132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7926..8514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8415..8434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8668..8685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8802..8823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8838..8843,8844..8861,8862..8868)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8876..8892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8881..8903
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8900..8916
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8900..8916
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8935..8955
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8937..8955
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8983..9209
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagagga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     2341 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2401 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2461 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2521 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2581 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2641 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2701 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2761 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2821 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2881 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2941 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3001 gggatcactc tcggcatgga cgagctgtac aagaccggat caacaagttt gtacaaaaaa
     3061 gcaggctggg gctcgcaggc cctgcccccg gggcccatgc agaccctcat ctttttcgac
     3121 atggaggcca ctggcttgcc cttctcccag cccaaggtca cggagctgtg cctgctggct
     3181 gtccacagat gtgccctgga gagccccccc acctctcagg ggccacctcc cacagttcct
     3241 ccaccaccgc gtgtggtaga caagctctcc ctgtgtgtgg ctccggggaa ggcctgcagc
     3301 cctgcagcca gcgagatcac aggtctgagc acagctgtgc tggcagcgca tgggcgtcaa
     3361 tgttttgatg acaacctggc caacctgctc ctagccttcc tgcggcgcca gccacagccc
     3421 tggtgcctgg tggcacacaa tggtgaccgc tacgacttcc ccctgctcca agcagagctg
     3481 gctatgctgg gcctcaccag tgctctggat ggtgccttct gtgtggatag catcactgcg
     3541 ctgaaggccc tggagcgagc aagcagcccc tcagaacacg gcccaaggaa gagctatagc
     3601 ctaggcagca tctacactcg cctgtatggg cagtcccctc cagactcgca cacggctgag
     3661 ggtgatgtcc tggccctgct cagcatctgt cagtggagac cacaggccct gctgcggtgg
     3721 gtggatgctc acgccaggcc tttcggcacc atcaggccca tgtatggggt cacagcctct
     3781 gctaggacca agccaagacc atctgctgtc acaaccactg cacacctggc cacaaccagg
     3841 aacactagtc ccagccttgg agagagcagg ggtaccaaaa ggatcttcct ccagtgaagg
     3901 accggtgaag taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     3961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4081 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4141 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4201 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4261 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4321 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4381 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4441 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4501 ttgggccgcc tccccgcctg gaattctgca gatatccggt tagtaatgag tttggaatta
     4561 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4621 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4681 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4741 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4801 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     4861 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     4921 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     4981 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     5041 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     5101 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     5161 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     5221 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     5281 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     5341 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     5401 tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     5461 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     5521 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5581 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5641 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5701 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     5761 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     5821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     5941 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6001 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6061 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6121 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6181 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6241 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6301 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6361 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6601 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6661 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6721 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6781 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6841 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6901 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6961 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7021 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7081 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7141 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7201 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7261 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7321 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7381 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7441 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7501 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7561 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7621 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7681 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7741 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7801 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7861 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7921 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7981 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8041 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8581 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8641 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8701 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8761 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8821 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8881 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     8941 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     9001 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     9061 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     9121 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     9181 tatttaagtg cctagctcga tacataaac
//
