LOCUS       sequence-347610-        6922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cry2PHR with N-terminal IBAR domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Wurz AI, Bunner WP, Szatmari EM, Hughes RM
  TITLE     CRY-BARs: Versatile light-gated molecular tools for the remodeling 
            of membrane architectures.
  JOURNAL   J Biol Chem. 2022 Aug 18;298(10):102388. doi: 
            10.1016/j.jbc.2022.102388.
  PUBMED    35987384
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-08-18"; volume: "298(10):102388. doi"; pages: " 
            10.1016/j.jbc.2022.102388"
COMMENT     Sequence Label: IBAR-Cry2PHR
FEATURES             Location/Qualifiers
     source          1..6922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             763..2256
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      2275..2284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2281..2991
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2426..2444)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2694..2713
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3114..3235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3151..3170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3205..3224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3242..3697)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3379..3400)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3591..3610
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3724..3828
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3830..4187
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3830..4021
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3853..3873)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4038..4173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4222..5016
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4222..5016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4276..4295)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4886..4905
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5204..5223)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5248..5295
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5624..6212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6113..6132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6387..6690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6691..6894
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6845..6865
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggaggctg tgattgagaa ggaatgcagc gcgctcggag gcctcttcca gaccatcatc
       61 agcgacatga aggggagcta tccagtttgg gaagatttca taaacaaagc aggaaagctg
      121 cagtcccagc ttcggacaac agtagtagca gcagctgcct tcttggacgc ctttcagaaa
      181 gtggctgaca tggccaccaa cacacgtggt gggaccaggg agattggatc tgctctcacc
      241 aggatgtgca tgaggcacag aagcattgaa gccaagctga ggcagttttc gagcgcttta
      301 attgattgtc tgataaaccc acttcaagaa cagatggaag aatggaagaa agtggccaac
      361 cagctggata aagaccacgc aaaagaatat aagaaagccc gccaagagat aaaaaagaag
      421 tcctcggata cgctgaaact gcagaagaaa gcaaaaaaag tggacactct tgggagaggt
      481 gatatccagc ctcagttgga cagtgctctc caagatgtca atgataagta tctcttattg
      541 gaagaaacag aaaagcaggc tgtccggaag gctttgattg aagaacgtgg ccgattctgt
      601 accttcatct ctatgctgcg gccagtgatt gaagaagaaa tctcaatgct aggggaaata
      661 acccaccttc agaccatctc ggaagatcta aaaagcctga ccatggaccc tcacaaactg
      721 ccctcctcaa gtgaacaggt gattctggac ctcgaggcca ccatgaagat ggacaaaaag
      781 actatagttt ggtttagaag agacctaagg attgaggata atcctgcatt agcagcagct
      841 gctcacgaag gatctgtttt tcctgtcttc atttggtgtc ctgaagaaga aggacagttt
      901 tatcctggaa gagcttcaag atggtggatg aaacaatcac ttgctcactt atctcaatcc
      961 ttgaaggctc ttggatctga cctcacttta atcaaaaccc acaacacgat ttcagcgatc
     1021 ttggattgta tccgcgttac cggtgctaca aaagtcgtct ttaaccacct ctatgatcct
     1081 gtttcgttag ttcgggacca taccgtaaag gagaagctgg tggaacgtgg gatctctgtg
     1141 caaagctaca atggagatct attgtatgaa ccgtgggaga tatactgcga aaagggcaaa
     1201 ccttttacga gtttcaattc ttactggaag aaatgcttag atatgtcgat tgaatccgtt
     1261 atgcttcctc ctccttggcg gttgatgcca ataactgcag cggctgaagc gatttgggcg
     1321 tgttcgattg aagaactagg gctggagaat gaggccgaga aaccgagcaa tgcgttgtta
     1381 actagagctt ggtctccagg atggagcaat gctgataagt tactaaatga gttcatcgag
     1441 aagcagttga tagattatgc aaagaacagc aagaaagttg ttgggaattc tacttcacta
     1501 ctttctccgt atctccattt cggggaaata agcgtcagac acgttttcca gtgtgcccgg
     1561 atgaaacaaa ttatatgggc aagagataag aacagtgaag gagaagaaag tgcagatctt
     1621 tttcttaggg gaatcggttt aagagagtat tctcggtata tatgtttcaa cttcccgttt
     1681 actcacgagc aatcgttgtt gagtcatctt cggtttttcc cttgggatgc tgatgttgat
     1741 aagttcaagg cctggagaca aggcaggacc ggttatccgt tggtggatgc cggaatgaga
     1801 gagctttggg ctaccggatg gatgcataac agaataagag tgattgtttc aagctttgct
     1861 gtgaagtttc ttctccttcc atggaaatgg ggaatgaagt atttctggga tacacttttg
     1921 gatgctgatt tggaatgtga catccttggc tggcagtata tctctgggag tatccccgat
     1981 ggccacgagc ttgatcgctt ggacaatccc gcgttacaag gcgccaaata tgacccagaa
     2041 ggtgagtaca taaggcaatg gcttcccgag cttgcgagat tgccaactga atggatccat
     2101 catccatggg acgctccttt aaccgtactc aaagcttctg gtgtggaact cggaacaaac
     2161 tatgcgaaac ccattgtaga catcgacaca gctcgtgagc tactagctaa agctatttca
     2221 agaacccgtg aagcacagat catgatcgga gcagcagccc gggatccacc ggtcgccacc
     2281 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     2341 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     2401 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     2461 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     2521 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     2581 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     2641 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     2701 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     2761 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     2821 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     2881 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     2941 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc
     3001 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3061 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     3241 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3301 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3361 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3421 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3481 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3541 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3601 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3661 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3841 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3961 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4021 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4081 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4141 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     4201 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4261 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4321 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4381 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     4441 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4501 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4561 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4621 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4681 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4741 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4801 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4861 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4921 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4981 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5041 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5101 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5161 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     5221 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     5281 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     5341 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     5401 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     5461 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5761 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6001 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6301 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     6361 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     6421 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     6481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     6541 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     6601 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     6661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     6721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     6781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     6841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     6901 gtgaaccgtc agatccgcta gc
//
