LOCUS       sequence-347621-        3933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A8_STOP
FEATURES             Location/Qualifiers
     source          1..3933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1490..1589)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1606..1625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1607..1625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1630..1646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1630..1646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1748..1767)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1759..2568
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1826..1845)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2715..3303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3204..3223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3457..3474
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3633..3660)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3752..3838)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3887..3909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3901..3918
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3902..3918
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc ccctggccgg
      121 gccgtggccg gcctgctgct gctggcagca gcaggactgg gaggagtggc cgagggccca
      181 ggcctggcct tttccgagga cgtgctgagc gtgttcggag caaacctgtc cctgtctgcc
      241 gcacagctgc agcacctgct ggagcagatg ggagcagcat ccagagtggg agtgccagag
      301 cctggccagc tgcacttcaa ccagtgtctg accgccgagg agatctttag cctgcacggc
      361 ttctccaatg ccacccagat cacaagctcc aagttttctg tgatctgccc cgccgtgctg
      421 cagcagctga acttccaccc gtgcgaggat aggccgaaac ataagacaag acctagccac
      481 tccgaagtgt ggggctacgg ctttctgtct gtgaccatca tcaatctggc cagcctgctg
      541 ggcctgatcc tgacaccact gatcaagaag tcctatttcc ccaagatcct gaccttcttt
      601 gtgggcctgg ccatcggcac actgttttct aacgccatct tccagctgat ccccgaggcc
      661 tttggcttcg accctaaggt ggatagctac gtggagaagg ccgtggccgt gtttggcggc
      721 ttctatctgc tgttcttttt cgagaggatg ctgaagatgc tgctgaagac ctacggccag
      781 aatggccaca cacactttgg caacgacaat ttcggccccc aggagaagac ccaccagcca
      841 aaggccctgc cagccatcaa cggagtgacc tgctatgcca atcctgccgt gacagaggcc
      901 aacggccaca tccactttga caacgtgagc gtggtgtctc tgcaggatgg caagaaggag
      961 ccttctagct gcacctgtct gaagggccca aagctgtccg agatcggcac catcgcctgg
     1021 atgatcacac tgtgcgacgc cctgcacaac ttcatcgatg gcctggccat cggcgcctct
     1081 tgtaccctga gcctgctgca gggcctgtct acaagcatcg ccatcctgtg cgaggagttt
     1141 ccccacgagc tgggcgactt cgtgatcctg ctgaacgccg gcatgtccac cagacaggcc
     1201 ctgctgttta atttcctgag cgcctgctcc tgttacgtgg gactggcctt cggcatcctg
     1261 gtgggcaaca attttgcccc aaatatcatc ttcgccctgg ccggcggcat gtttctgtat
     1321 atcagcctgg ccgacatgtt ccccgagatg aacgatatgc tgagggagaa ggtgaccggc
     1381 cgcaagaccg acttcacctt cttcatgatc cagaatgccg gcatgctgac cggcttcaca
     1441 gccatcctgc tgatcacact gtacgccggc gagatcgagc tggagtaaga cccagctttc
     1501 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1561 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1621 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1681 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1741 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1801 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1861 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1921 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1981 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2041 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2101 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2161 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2221 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2281 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2341 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2401 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2461 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2521 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2581 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2641 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2821 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3421 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3481 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3601 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3661 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3721 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3781 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3841 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     3901 tgtaaaacga cggccagtct taagctcggg ccc
//
