LOCUS       sequence-347867-        4026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC43A3_STOP
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1420..1431)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1583..1682)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1699..1718)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1700..1718)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1723..1739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1723..1739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1841..1860)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1852..2661
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1919..1938)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2808..3396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3297..3316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3550..3567
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3726..3753)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3845..3931)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3980..4002
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3994..4011
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3995..4011
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc aggacaggga
      121 ctgccactgc acgtggccac cctgctgaca ggactgctgg agtgcctggg attcgcaggc
      181 gtgctgtttg gatggccatc cctggtgttc gtgtttaaga acgaggacta cttcaaggat
      241 ctgtgcggac cagacgcagg acctatcgga aatgcaaccg gacaggcaga ctgtaaggca
      301 caggatgagc ggttctctct gatctttaca ctgggcagct ttatgaacaa tttcatgacc
      361 tttcccacag gctacatctt cgatcggttt aagaccacag tggccagact gatcgccatc
      421 ttcttttata ccacagccac cctgatcatc gccttcacaa gcgccggatc cgccgtgctg
      481 ctgtttctgg ccatgcctat gctgaccatc ggcggcatcc tgttcctgat cacaaacctg
      541 cagatcggca atctgtttgg tcaacataga tccaccatca taaccctgta caacggcgcc
      601 ttcgacagct cctctgccgt gtttctgatc atcaagctgc tgtatgagaa gggcatcagc
      661 ctgagagcct ccttcatctt tatcagcgtg tgcagcacct ggcacgtggc caggacattc
      721 ctgctgatgc caaggggaca catcccatac ccactgcccc ctaactacag ctatggcctg
      781 tgccctggca atggcaccac aaaggaggag aaggagaccg ccgagcacga gaatagggag
      841 ctgcagagca aggagttcct gtccgccaag gaggagacac caggagcagg acagaagcag
      901 gagctgcgct ccttttggtc ttacgccttc tcccggagat ttgcctggca cctggtgtgg
      961 ctgtctgtga tccagctgtg gcactatctg ttcatcggca ccctgaactc cctgctgaca
     1021 aatatggcag gaggcgacat ggcaagggtg tctacctaca caaacgcctt cgcctttacc
     1081 cagtttggcg tgctgtgcgc cccttggaat ggcctgctga tggatcggct gaagcagaag
     1141 tatcagaagg aggccagaaa gaccggaagc tccacactgg cagtggccct gtgctctacc
     1201 gtgccaagcc tggccctgac aagcctgctg tgcctgggat tcgcactgtg cgcatccgtg
     1261 cctatcctgc cactgcagta cctgaccttc atcctgcagg tcatctctcg cagctttctg
     1321 tatggctcta acgccgcctt cctgaccctg gcctttccta gcgagcactt cggcaagctg
     1381 tttggactgg tcatggcact gtccgccgtg gtgtctctgc tgcagttccc aatctttacc
     1441 ctgatcaagg gcagcctgca gaacgatccc ttctacgtga atgtgatgtt tatgctggcc
     1501 atcctgctga ccttctttca ccctttcctg gtgtataggg agtgtcgcac atggaaggag
     1561 tccccatctg ccatcgccta agacccagct ttcttgtaca aagttggcat tataagaaag
     1621 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1681 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1741 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1801 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1861 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1921 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1981 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2041 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2101 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2161 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2221 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2281 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2341 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2401 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2461 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2521 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2581 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2641 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2701 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2941 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3481 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3541 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3601 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3661 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3721 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3781 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3841 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3901 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3961 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4021 gggccc
//
