LOCUS       sequence-34787-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     Cookson Lab Plasmids
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6-DJ1-V5-E18Q
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..53)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    106..130
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(728..752)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taaccggtac gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac
       61 cgcgggccct ctagactcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag
      121 aaagctgggt cgaattcgcc cttcttgtct ttaagaacaa gtggagcctt cacttgagcc
      181 gccacctcct tgccattcag ggcttcaaca attgcaagcg caaactcgaa gctggtccca
      241 ggcccccggc ttgtaagaat caggccgtct ttttccacac gattctcaga gtaggtgtaa
      301 tgacctccat tcatcatttt gtctttagca agagggtgtg ttgtaacttt acttccaaaa
      361 cctatttcat gagccaacag agcagtagga cctgcacaga tggcggctat caggcccttc
      421 cggttttcct gctccttcag tatctccttc acagcagcag actcagataa attctgtgcg
      481 cccagattac ctcctggtag aaccaccaca tcatatggtc cctctttttt tgcatcttca
      541 aggctggcat caggacaaat gaccacatca cggctacact gtactgggtc ttttccagcc
      601 aggcctgcaa cggtgacctt aatcccagct cgcctcatga catctacagg gatgaccgtc
      661 tgcatttcct ctgctccttt agccaggatg accagagctc ttttggaagc cataagggcg
      721 aattcggagc ctgctttttt gtacaaactt gttgatatct gcagaattcc accacactgg
      781 actagtggat cctctagagt cggtgtcttc tatggaggtc aaaacagcgt gg
//
