LOCUS       sequence-348238-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, N439K).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-N439K
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             202..996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(256..275)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     866..885
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1170..1291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1207..1226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1261..1280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1340..1356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1340..1356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1353..1375)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1364..1380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1388..1394,1395..1412,1413..1418))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1433..1454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1571..1588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1742..2330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1822..1841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2501..3292,3293..3361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3124..3143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3362..3466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3541..3560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3732..4111
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4112..4315
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4266..4286
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4360..4379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4360..4378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8257..8274)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8263..8487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8533..8961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8620..8639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8830..8851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8970..8990)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8975..9169,1..135)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9155..9169,1..121)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
       61 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      121 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
      181 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
      241 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      301 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      361 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
      421 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      481 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      541 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      601 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      661 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      721 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
      781 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      841 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
      901 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
      961 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     1021 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     1081 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     1141 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     1201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1261 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     1321 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1381 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1441 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1501 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2161 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3481 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     3541 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     3601 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     3661 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     3721 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     3781 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3841 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3901 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3961 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     4021 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     4081 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     4141 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     4201 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     4261 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     4321 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
     4381 gacccaagct ggctagcatg tttgtcttcc tggtcctgct gcctctggtc tcgtctcagt
     4441 gcgtgaacct gactactaga acccagctgc ctcctgccta tactaactcc ttcacccgcg
     4501 gcgtgtacta cccagacaag gtgttccgca gctccgtgct gcactccacc caggatctgt
     4561 tcctgccctt cttcagcaac gtgacctggt tccacgccat ccacgtgagc ggcaccaatg
     4621 gcaccaagcg gttcgacaat cccgtgctgc cattcaacga tggcgtgtac ttcgcctcca
     4681 ccgagaagag caacatcatc cgcggctgga tcttcggcac caccctggac tccaagaccc
     4741 agagcctgct gatcgtgaac aatgccacca acgtggtcat caaggtgtgc gagttccagt
     4801 tctgcaatga tccattcctg ggcgtgtact accacaagaa caataagtcc tggatggaga
     4861 gcgagttccg cgtgtacagc tccgccaaca attgcacctt cgagtacgtg tcccagccct
     4921 tcctgatgga cctggagggc aagcagggca atttcaagaa cctgcgcgag ttcgtgttca
     4981 agaatatcga tggctacttc aagatctact ccaagcacac ccccatcaac ctggtgcgcg
     5041 acctgccaca gggcttcagc gccctggagc cactggtgga tctgccaatc ggcatcaaca
     5101 tcaccaggtt ccagaccctg ctggccctgc accgcagcta cctgacccca ggcgacagct
     5161 ccagcggatg gaccgctgga gctgctgcct actacgtggg ctacctgcag ccccgcacct
     5221 tcctgctgaa gtacaacgag aatggcacca tcaccgacgc cgtggattgc gccctggatc
     5281 cactgtccga gacaaagtgc accctgaaga gcttcaccgt ggagaagggc atctaccaga
     5341 cctccaattt ccgcgtgcag ccaaccgaga gcatcgtgcg cttccccaat atcaccaacc
     5401 tgtgcccatt cggcgaggtg ttcaacgcta ccaggttcgc cagcgtgtac gcttggaatc
     5461 gcaagcgcat ctccaactgc gtggccgact acagcgtgct gtacaactcc gccagcttct
     5521 ccaccttcaa gtgctacggc gtgtccccca ccaagctgaa tgatctgtgc ttcaccaacg
     5581 tgtacgccga tagcttcgtg atcaggggcg acgaggtgcg ccagatcgct ccaggacaga
     5641 ccggcaagat cgctgactac aattacaagc tgcccgacga tttcaccggc tgcgtgatcg
     5701 cctggaactc caagaatctg gatagcaaag tgggcggcaa ctacaattac ctgtaccgcc
     5761 tgttccgcaa gtccaatctg aagccattcg agcgcgacat ctccaccgag atctaccagg
     5821 ctggaagcac cccatgcaat ggagtggagg gcttcaactg ctacttcccc ctgcagagct
     5881 acggcttcca gccaaccaac ggagtgggat accagccata cagggtggtg gtgctgtcct
     5941 tcgagctgct gcacgctcca gctaccgtgt gcggaccaaa gaagagcacc aatctggtga
     6001 agaacaagtg cgtgaacttc aatttcaacg gcctgaccgg aaccggcgtg ctgaccgagt
     6061 ccaacaagaa gttcctgcca ttccagcagt tcggaaggga catcgctgat accaccgacg
     6121 ccgtgcgcga cccacagacc ctggagatcc tggatatcac cccatgctcc ttcggcggcg
     6181 tgagcgtgat caccccagga accaatacca gcaaccaggt ggccgtgctg taccagggcg
     6241 tgaattgcac cgaggtgcca gtggctatcc acgctgatca gctgacccca acctggcgcg
     6301 tgtacagcac cggatccaac gtgttccaga cccgcgccgg atgcctgatc ggagctgagc
     6361 acgtgaacaa ttcctacgag tgcgacatcc caatcggagc tggaatctgc gccagctacc
     6421 agacccagac caactcccca aggagggctc gcagcgtggc cagccagtcc atcatcgcct
     6481 acaccatgtc cctgggcgcc gagaatagcg tggcctacag caacaattcc atcgccatcc
     6541 caaccaactt caccatctcc gtgaccaccg agatcctgcc cgtgtccatg accaagacca
     6601 gcgtggactg caccatgtac atctgcggcg attccaccga gtgcagcaac ctgctgctgc
     6661 agtacggcag cttctgcacc cagctgaatc gcgccctgac cggaatcgct gtggagcagg
     6721 ataagaacac ccaggaggtg ttcgcccagg tgaagcagat ctacaagacc cccccaatca
     6781 aggacttcgg cggcttcaat ttcagccaga tcctgcccga tccaagcaag ccctccaagc
     6841 gcagcttcat cgaggacctg ctgttcaaca aggtgaccct ggccgatgcc ggcttcatca
     6901 agcagtacgg cgattgcctg ggcgacatcg ctgcccgcga cctgatctgc gcccagaagt
     6961 tcaatggcct gaccgtgctg ccaccactgc tgaccgatga gatgatcgct cagtacacct
     7021 ccgccctgct ggccggaacc atcaccagcg gatggacctt cggcgctgga gccgccctgc
     7081 agatcccctt cgccatgcag atggcctacc gcttcaacgg catcggcgtg acccagaatg
     7141 tgctgtacga gaaccagaag ctgatcgcca atcagttcaa ctccgccatc ggcaagatcc
     7201 aggactccct gtccagcacc gccagcgccc tgggcaagct gcaggatgtg gtgaatcaga
     7261 acgcccaggc cctgaatacc ctggtgaagc agctgtccag caacttcggc gccatctcca
     7321 gcgtgctgaa tgatatcctg agccgcctgg acaaggtgga ggctgaggtg cagatcgata
     7381 ggctgatcac cggccgcctg cagtccctgc agacctacgt gacccagcag ctgatcaggg
     7441 ctgctgagat cagggccagc gccaatctgg ctgctaccaa gatgtccgag tgcgtgctgg
     7501 gacagagcaa gagggtggac ttctgcggca agggctacca cctgatgtcc ttcccacaga
     7561 gcgccccaca cggagtggtg ttcctgcacg tgacctacgt gccagcccag gagaagaact
     7621 tcaccaccgc tccagctatc tgccacgatg gcaaggctca cttcccacgc gagggcgtgt
     7681 tcgtgtccaa cggcacccac tggttcgtga cccagcgcaa tttctacgag ccccagatca
     7741 tcaccaccga caataccttc gtgagcggca actgcgacgt ggtcatcgga atcgtgaaca
     7801 ataccgtgta cgatcccctg cagccagagc tggactcctt caaggaggag ctggataagt
     7861 acttcaagaa tcacaccagc cccgacgtgg atctgggcga catctccggc atcaatgcca
     7921 gcgtggtgaa catccagaag gagatcgacc gcctgaacga ggtggccaag aatctgaacg
     7981 agtccctgat cgatctgcag gagctgggca agtacgagca gtacatcaag tggccatggt
     8041 acatctggct gggcttcatc gccggcctga tcgccatcgt gatggtgacc atcatgctgt
     8101 gctgcatgac ctcctgctgc agctgcctga agggctgctg ctcctgcggc agctgctgca
     8161 agttcgatga ggacgatagc gagcccgtgc tgaagggcgt caaactgcac tatacatagc
     8221 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8281 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8341 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8401 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8461 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     8521 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8581 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8641 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8701 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8761 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8821 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8881 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8941 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     9001 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     9061 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9121 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaact
//
