LOCUS       sequence-34828-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Murakoshi H, Wang H, Yasuda R
  TITLE     Local, persistent activation of Rho GTPases during plasticity of 
            single dendritic spines.
  JOURNAL   Nature. 2011 Apr 7;472(7341):100-4. doi: 10.1038/nature09823. Epub 
            2011 Mar 20.
  PUBMED    21423166
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature09823"; journalName: "Nature"; date: "2011-04-7- 7"; 
            volume: "472"; issue: "7341"; pages: "100-4"
COMMENT     Sequence Label: mEGFP-RhoA-C1
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..628
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     polyA_signal    758..879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(795..814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     849..868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagggatgg ctgccatccg
       61 gaagaaactg gtgattgttg gtgatggagc ctgtggaaag acatgcttgc tcatagtctt
      121 cagcaaggac cagttcccag aggtgtatgt gcccacagtg tttgagaact atgtggcaga
      181 tatcgaggtg gatggaaagc aggtagagtt ggctttgtgg gacacagctg ggcaggaaga
      241 ttatgatcgc ctgaggcccc tctcctaccc agataccgat gttatactga tgtgtttttc
      301 catcgacagc cctgatagtt tagaaaacat cccagaaaag tggaccccag aagtcaagca
      361 tttctgtccc aacgtgccca tcatcctggt tgggaacaag aaggatcttc ggaatgatga
      421 gcacacaagg cgggagctag ccaagatgaa gcaggagccg gtgaaacctg aagaaggcag
      481 agatatggca aacaggattg gcgcttttgg gtacatggag tgttcagcaa agaccaaaga
      541 tggagtgaga gaggtttttg aaatggctac gagagctgct ctgcaagcta gacgtgggaa
      601 gaaaaaatct gggtgccttg tcttgtgagg atccaccgga tctagataac tgatcataat
      661 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      721 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
      901 atattttgtt aaaatt
//
