LOCUS       sequence-348360-        3851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus oocyte expression vector containing YER039C. Contains T7 RNA
            polymerase binding site, and adds polyA tail to YER039C. Used for 
            generation of mRNA, for injection into xenopus laevis oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Moller-Hansen I, Saez-Saez J, van der Hoek SA, Dyekjaer JD, 
            Christensen HB, Wright Muelas M, O'Hagan S, Kell DB, Borodina I
  TITLE     Deorphanizing solute carriers in Saccharomyces cerevisiae for 
            secondary uptake of xenobiotic compounds.
  JOURNAL   Front Microbiol. 2024 Apr 12;15:1376653. doi: 
            10.3389/fmicb.2024.1376653.
  PUBMED    38680917
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2024-04-12"; volume: "15:1376653. doi"; pages: "
            10.3389/fmicb.2024.1376653"
COMMENT     Sequence Label: pCFB8457
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(88..106)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(89..106)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(124..140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(124..140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(137..159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(172..178,179..196,197..202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(355..372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(526..1114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(606..625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1285..2076,2077..2145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1908..1927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2146..2250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2318..2336
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2374..2396)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2496..2515
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2709..2731
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2723..2740
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2724..2740
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2747..2766
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2747..2765
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2797..2839
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           3723..3848
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 ctaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccc cccccccccc cccctgcagg
       61 cggccgcctc gaggctagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc
      121 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      241 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      301 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      361 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      961 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1261 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1321 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1381 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1441 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1501 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1561 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1621 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1681 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1741 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1801 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1861 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1921 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1981 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2041 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2101 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2161 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2281 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2341 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2461 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     2521 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2581 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2641 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2701 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     2761 atagggcgaa ttaattcgag ctcggtaccc agcttgcttg ttctttttgc agaagctcag
     2821 aataaacgct caactttggc agatcaattc ccggggctga ggcttaatat gatttatacg
     2881 tcgtcaaaga gcttacaata ccttgccgtg cccatctaca caattttcaa gaatctgacg
     2941 atcattctca ttgcctatgg cgaggtcctc ttctttggcg gaaaagtcac ttctatggag
     3001 ttgacgtcat ttattatgat ggtcctttca tctgtagtgg ccacctgggg cgatcagcag
     3061 gccattgcaa tcaaagcatc atctttggag gatctcgatc aggaacttgt tgagtcaacc
     3121 atctttgtgt agaatccggg gtacctctgg atgttcacca attgtatttc gtctgcattg
     3181 ttcgttttga ttatgcggaa gagaattcgg ctgactaact ttaaagacta tgatactatg
     3241 ttttataaca acgttctggc attgcctttg ctgctagtct tttccttcat tatggaagac
     3301 tggtccacaa agaatttgtc tgtgaacttg tccgctgact cactggcagc aatggtcatc
     3361 agcgggctca tgtccgtggg gatatcgtac tgttccggct ggtgtgttcg tgtaacttcc
     3421 tctacaacgt attctatggt tggtgctttg aacaaattgc ccatagcgtt ggcgggattg
     3481 gtgttctttg acgctccaaa aaactttttg tccttctttt ctatctttct aggatttctg
     3541 tctggcctgc tgtacgctgt ggctaaacag aaaaagatac aacagcaaaa agttctagca
     3601 gctactcttg agaagtgaat taaacctcag cgaattcgtg agaaagcttg ggaataggcc
     3661 tttcagatct gcccgagacc atggatgaag tatctagaaa tagcttgatc tggttaccac
     3721 taaaccagcc tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact
     3781 ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt
     3841 ttcttcacat t
//
