LOCUS       sequence-348406-        8121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of sgRNA targeting LINE-1 and TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C
  TITLE     Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts 
            retrotransposition.
  JOURNAL   EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.
  PUBMED    35856394
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 20:e54458. doi: 10.15252/embr.202154458."
COMMENT     Sequence Label: pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1
FEATURES             Location/Qualifiers
     source          1..8121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             151..195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    493..610
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        693..941
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     693..713
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     864..883
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        961..1036
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1059..1558
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1094..1117)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1491..1510
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1598..2194
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1598..1617)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2094..2114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2213..2266
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2267..2968
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(3323..3340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3329..3553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3579..3599)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3579..3597)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3618..3634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3618..3634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3631..3653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3642..3658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3666..3672,3673..3690,3691..3696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3711..3732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3849..3866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4020..4608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4100..4119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4779..5570,5571..5639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5402..5421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5640..5744)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5770..6225)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5907..5928)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6119..6138
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6352..6374
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6366..6383
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6367..6383
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6393..6412
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6393..6411
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6482..6785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6787..6985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6940..6960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7003..7183
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7230..7355
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7854..8087
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
       61 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
      121 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
      181 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
      241 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
      301 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
      361 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
      421 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
      481 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      541 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      601 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag taccgggccc
      661 tacgcgttac tcgagccaag gtcgggcagg aagagggcct atttcccatg attccttcat
      721 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
      781 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
      841 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      901 atttcttggc tttatatatc ttgtggaaag gacgaaacac ccagaggaca ggtgcccgcc
      961 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1021 ggcaccgagt cggtgctttt tttggatcca attctaccgg gtaggggagg cgcttttccc
     1081 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     1141 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     1201 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     1261 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     1321 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     1381 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     1441 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     1501 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     1561 ctgcatccat ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt
     1621 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     1681 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     1741 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     1801 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     1861 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     1921 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     1981 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     2041 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     2101 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     2161 gcgcacctgg tgcatgaccc gcaagcccgg tgccggcggc gggtccggag gagagggcag
     2221 aggaagtctc ctaacatgcg gtgacgtgga ggagaatcct ggcccaatga gcgagctgat
     2281 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     2341 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     2401 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     2461 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     2521 tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc tgaccgctac
     2581 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     2641 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     2701 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     2761 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     2821 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     2881 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     2941 tagcaaactg gggcacaagc ttaattgagc ggccgctagg tacctttaag accaatgact
     3001 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3061 attcactccc aaagaagtca agatctgctt tttgcctgta ctgggtctct ctggttagac
     3121 cagagtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3181 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3241 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3301 gcagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3361 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3421 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3481 gtggggcagg acagcaaggg ggaggattgg gaagtcaata gcaggcatgc tggggatgcg
     3541 gtgggctcta tgggcggccg ttaattaagc ttttgttccc tttagtgagg gttaattgcg
     3601 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3661 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3721 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3781 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3841 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3901 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3961 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4441 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4921 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5761 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5821 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5881 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5941 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6001 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6061 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6121 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6181 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     6241 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     6301 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     6361 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattga
     6421 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     6481 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     6541 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     6601 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     6661 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6721 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     6781 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6841 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6901 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6961 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     7021 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7081 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7141 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7201 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     7261 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     7321 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     7381 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     7441 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     7501 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     7561 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     7621 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     7681 ccgcacagca agcggccggc cgcgctgatc ttcagacctg gaggaggaga tatgagggac
     7741 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     7801 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     7861 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     7921 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     7981 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8041 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8101 tgctctggaa aactcatttg c
//
