LOCUS       sequence-349091-       14013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector mediating bicistronic expression of the 4 genes of
            YFV-17D (C-prM-E-NS1) and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: FUW-C-prM-E-NS1
FEATURES             Location/Qualifiers
     source          1..14013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(17..121)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(196..215)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        387..766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        768..966
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     921..941
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             984..1164
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1211..1336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1829..2062
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2247..2291
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2589..2706
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2771..3982
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3819..3838
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    7442..7993
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7586..7603)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             join(8005..8007,8008..8010,8011..8724)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8050..8071)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8311..8330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8658..8679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8854..9442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8907..8927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9325..9336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9445..9461)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9446..9462)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9967..10147
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10173..10190)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10179..10403
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10449..10877
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10536..10555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10746..10767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10886..10906)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10891..11220
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11071..11206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11133..11152
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11268..11315
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11334..11708
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11838..11959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11875..11894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11929..11948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12008..12024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12008..12024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12021..12043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12032..12048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12056..12062,12063..12080,12081..12086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12101..12122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12239..12256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12410..12998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12490..12509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13169..13960,13961..14013,1..16))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13792..13811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
       61 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      121 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
      181 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
      241 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
      301 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
      361 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      421 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      481 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      541 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      601 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      661 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      721 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      781 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      841 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      901 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      961 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     1021 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1081 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     1141 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     1201 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     1261 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     1321 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1381 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1441 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1501 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1561 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1621 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1681 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1741 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1801 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1861 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1921 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1981 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     2041 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     2101 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     2161 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     2221 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     2281 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     2341 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2401 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2461 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2521 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2581 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2641 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2701 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
     2761 ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     2821 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     2881 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     2941 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     3001 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     3061 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     3121 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     3181 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     3241 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     3301 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     3361 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     3421 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     3481 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     3541 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     3601 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     3661 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     3721 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     3781 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     3841 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     3901 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     3961 gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatcaccgg
     4021 tgccaccatg tccggtcgta aagcccaggg caaaacccta ggcgtaaata tggtccgacg
     4081 cggagtccgc tcattgtcga acaaaataaa acaaaaaaca aaacaaattg gaaacagacc
     4141 tggaccttca agaggtgttc aaggatttat ctttttcttt ttgttcaaca ttttgactgg
     4201 aaaaaagatc acagcccacc taaagaggtt gtggaaaatg ctggacccaa gacaaggctt
     4261 ggctgttcta aggaaagtca agagagtggt ggccagtttg atgagaggat tgtcctcaag
     4321 gaaacgccgt tcccatgatg ttctgactgt gcaattccta attttgggaa tgctgttgat
     4381 gacgggtgga gtgaccttgg tgcggaaaaa cagatggttg ctcctaaatg tgacatctga
     4441 ggacctcggg aaaacattct ctgtgggcac aggcaactgc acaacaaaca ttttggaagc
     4501 caagtactgg tgcccagact caatggaata caactgtccc aatctcagtc caagagagga
     4561 gccagatgac attgattgct ggtgctatgg ggtggaaaac gttagagtcg catatggtaa
     4621 gtgtgactca gcaggcaggt ctaggaggtc aagaagggcc attgacttgc ctacgcatga
     4681 aaaccatggt ttgaagaccc ggcaagaaaa atggatgact ggaagaatgg gtgaaaggca
     4741 actccaaaag attgagagat ggttcgtgag gaaccccttt tttgcagtga cggctctgac
     4801 cattgcctac cttgtgggaa gcaacatgac gcaacgagtc gtgattgccc tactggtctt
     4861 ggctgttggt ccggcctact cagctcactg cattggaatt actgacaggg atttcattga
     4921 gggggtgcat ggaggaactt gggtttcagc taccctggag caagacaagt gtgtcactgt
     4981 tatggcccct gacaagcctt cattggacat ctcactagag acagtagcca ttgatagacc
     5041 tgctgaggtg aggaaagtgt gttacaatgc agttctcact catgtgaaga ttaatgacaa
     5101 gtgccccagc actggagagg cccacctagc cgaagagaac gaaggggaca atgcgtgcaa
     5161 gcgcacttat tctgatagag gctggggcaa tggctgtggc ctatttggga aagggagcat
     5221 tgtggcatgc gccaaattca cttgtgccaa atccatgagt ttgtttgagg ttgatcagac
     5281 caaaattcag tatgtcatca gagcacaatt gcatgtaggg gccaagcagg aaaattggaa
     5341 taccgacatt aagactctca agtttgatgc cctgtcaggc tcccaggaag tcgagttcat
     5401 tgggtatgga aaagctacac tggaatgcca ggtgcaaact gcggtggact ttggtaacag
     5461 ttacatcgct gagatggaaa cagagagctg gatagtggac agacagtggg cccaggactt
     5521 gaccctgcca tggcagagtg gaagtggcgg ggtgtggaga gagatgcatc atcttgtcga
     5581 atttgaacct ccgcatgccg ccactatcag agtactggcc ctgggaaacc aggaaggctc
     5641 cttgaaaaca gctcttactg gcgcaatgag ggttacaaag gacacaaatg acaacaacct
     5701 ttacaaacta catggtggac atgtttcttg cagagtgaaa ttgtcagctt tgacactcaa
     5761 ggggacatcc tacaaaatat gcactgacaa aatgtttttt gtcaagaacc caactgacac
     5821 tggccatggc actgttgtga tgcaggtgaa agtgtcaaaa ggagccccct gcaggattcc
     5881 agtgatagta gctgatgatc ttacagcggc aatcaataaa ggcattttgg ttacagttaa
     5941 ccccatcgcc tcaaccaatg atgatgaagt gctgattgag gtgaacccac cttttggaga
     6001 cagctacatt atcgttggga gaggagattc acgtctcact taccagtggc acaaagaggg
     6061 aagctcaata ggaaagttgt tcactcagac catgaaaggc gtggaacgcc tggccgtcat
     6121 gggagacacc gcctgggatt tcagctccgc tggagggttc ttcacttcgg ttgggaaagg
     6181 aattcatacg gtgtttggct ctgcctttca ggggctattt ggcggcttga actggataac
     6241 aaaggtcatc atgggggcgg tacttatatg ggttggcatc aacacaagaa acatgacaat
     6301 gtccatgagc atgatcttgg taggagtgat catgatgttt ttgtctctag gagttggggc
     6361 ggatcaagga tgcgccatca actttggcaa gagagagctc aagtgcggag atggtatctt
     6421 catatttaga gactctgatg actggctgaa caagtactca tactatccag aagatcctgt
     6481 gaagcttgca tcaatagtga aagcctcttt tgaagaaggg aagtgtggcc taaattcagt
     6541 tgactccctt gagcatgaga tgtggagaag cagggcagat gagatcaatg ccatttttga
     6601 ggaaaacgag gtggacattt ctgttgtcgt gcaggatcca aagaatgttt accagagagg
     6661 aactcatcca ttttccagaa ttcgggatgg tctgcagtat ggttggaaga cttggggtaa
     6721 gaaccttgtg ttctccccag ggaggaagaa tggaagcttc atcatagatg gaaagtccag
     6781 gaaagaatgc ccgttttcaa accgggtctg gaattctttc cagatagagg agtttgggac
     6841 gggagtgttc accacacgcg tgtacatgga cgcagtcttt gaatacacca tagactgcga
     6901 tggatctatc ttgggtgcag cggtgaacgg aaaaaagagt gcccatggct ctccaacatt
     6961 ttggatggga agtcatgaag taaatgggac atggatgatc cacaccttgg aggcattaga
     7021 ttacaaggag tgtgagtggc cactgacaca tacgattgga acatcagttg aagagagtga
     7081 aatgttcatg ccgagatcaa tcggaggccc agttagctct cacaatcata tccctggata
     7141 caaggttcag acgaacggac cttggatgca ggtaccacta gaagtgaaga gagaagcttg
     7201 cccagggact agcgtgatca ttgatggcaa ctgtgatgga cggggaaaat caaccagatc
     7261 caccacggat agcgggaaag ttattcctga atggtgttgc cgctcctgca caatgccgcc
     7321 tgtgagcttc catggtagtg atgggtgttg gtatcccatg gaaattaggc caaggaaaac
     7381 gcatgaaagc catctggtgc gctcctgggt tacagcttga ccggttcagc tagcacccct
     7441 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     7501 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     7561 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     7621 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     7681 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     7741 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     7801 gaaagagtca aatggctctc ctaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     7861 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     7921 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     7981 cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg
     8041 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc
     8101 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     8161 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     8221 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     8281 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     8341 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     8401 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     8461 atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     8521 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     8581 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     8641 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     8701 ggcatggacg agctgtacaa gtaatgaatt aattaagaat tatcaccgct tctattcagc
     8761 cagtaaggcc tgtctttaat ggcctccggc atgagacact ttctagagtc gacctgcagc
     8821 caagcttatc gattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     8881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9241 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9361 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9421 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     9481 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9541 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9601 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9661 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9721 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     9781 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     9841 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     9901 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     9961 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10021 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10081 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10141 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
    10201 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    10261 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    10321 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    10381 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    10441 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10501 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10561 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10621 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10681 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10741 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10801 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10861 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10921 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10981 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    11041 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    11101 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
    11161 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    11221 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
    11281 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
    11341 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
    11401 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
    11461 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
    11521 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
    11581 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
    11641 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
    11701 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    11761 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    11821 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    11881 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11941 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
    12001 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    12061 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    12121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    12181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    12241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12361 aaggccaaca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    13081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    13141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    13201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    13261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    13321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    13381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    13441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    13501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    13561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    13621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    13681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    13741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    13801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    13861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    13921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    13981 atgccgcaaa aaagggaata agggcgacac gga
//
