LOCUS       sequence-349371-        4442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human MATR3 at the C-terminus
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4442)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MATR3_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..4442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(500..1291,1292..1360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1123..1142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1361..1465)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1533..1551
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1589..1611)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1711..1730
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1924..1946
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1938..1955
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1939..1955
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2436..3323
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     complement(3781..3797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3781..3797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3794..3816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3805..3821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3829..3835,3836..3853,3854..3859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3874..3895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4012..4029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4183..4442,1..329))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4263..4282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
       61 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      181 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc
      661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
      721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
      781 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
      841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
      901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
      961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1081 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     1501 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     1561 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     1621 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     1681 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     1741 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     1801 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1861 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1921 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
     1981 tgcatctaga aaactctccc tcaaaaaaaa aagaataaaa gtgcatgtca tttaaaccca
     2041 tagttcttga atagtgtcag ttcagtgtga ctgtggtctt tgttttctca tctctaaaaa
     2101 ttagggttaa ttataacatt tgaaataccc attttatacc ttttaccatt ttttccccgt
     2161 gccttattgt aatgtcctga aagtttttgt gtgctaatga ggattaacta attgttgaaa
     2221 atattgacaa aattatagtt taagtcccta aacttggata taggatattt gattttggaa
     2281 ttaatccatt ttgctgcatt tctcttaggt gacttaatgg ctgtaattct ctttctttat
     2341 agaaatttct gaataaattg gcagaagaac gcagacagaa gaaggaaact ggtggaggag
     2401 gttctggagg cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct
     2461 tcgatcctca ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca
     2521 gagatggaac ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga
     2581 acatcatccc tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg
     2641 gcaagagcga taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg
     2701 cctttattga ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg
     2761 ccctgggatt ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg
     2821 aattcatcag gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc
     2881 aggccttcag aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg
     2941 agggcaccct gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca
     3001 gagagccctt tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc
     3061 ccattgccgg cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc
     3121 atcagagccc cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg
     3181 ctgaagctgc cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg
     3241 gcctgaatct gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc
     3301 tgagcaccct ggaaatcagc ggataagatg tgcaaggaga tttaatgatt tcaaagaaaa
     3361 taatggttct ttgtttttaa tgttaacctt ttttaaatac aatactgata gttagaagaa
     3421 aactattgta ctcttttgtt ttagtggaga aataatagat gtctgttcat gtgttaagtg
     3481 ttatagcaaa aaaaatacac atatggttaa gttaatgaat agtttttgtt ttatcagaat
     3541 ggcaacagac agaagtactt tgtagagatt gacttcctaa gctacttaag acaacttgca
     3601 ccactaagaa aaaaatgtag aaccatttgg aaaaatgaaa tttagtagtt ccaagtttca
     3661 aagaaatgtc aacattttat tccattcaat aaagaacaaa accaatagtg tttttattac
     3721 tttcatggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg
     3781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3961 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4021 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4081 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4141 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4201 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4261 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4321 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4381 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4441 cg
//
