LOCUS       sequence-349963-        9639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a 
            lentivirus backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia
            BZ, Baker D, Lavis LD, Cohen AE
  TITLE     Time-tagged ticker tapes for intracellular recordings.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7.
  PUBMED    36593408
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7."
COMMENT     Sequence Label: DL015:CMV::iPAK4
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3209..3232
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4331..4919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4384..4404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4802..4813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4922..4938)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4923..4939)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5444..5624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5650..5667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5656..5880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5926..6354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6013..6032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6223..6244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6363..6383)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6368..6697
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6548..6683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6610..6629
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6745..6792
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6811..7185
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7315..7436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7352..7371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7406..7425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7485..7501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7485..7501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7498..7520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7509..7525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7533..7539,7540..7557,7558..7563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7578..7599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7716..7733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7887..8475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7967..7986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8646..9437,9438..9506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9269..9288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9507..9611)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatgga ctacaaggac gacgatgata agtccggatc
     3241 cgaagcagag gactggacag cagccctact gaacaggggt cgcagtcgcc agcccctggt
     3301 actaggggac aattgctttg ctgacttggt gcacaactgg atggagctgc ctgaggaatt
     3361 ccccgccgcc cctgctgttc ctgggccccc tggcccccgc tcaccacagc gggagccaca
     3421 gcgagtatcc catgagcagt tccgggctgc cctgcagctg gtggtggacc caggcgaccc
     3481 ccgctcctac ctggacaact tcatcaagat tggcgagggc tccacgggca tcgtgtgcat
     3541 cgccaccgtg cgcagctcgg gcaagctggt ggccgtcaag aagatggacc tgcgcaagca
     3601 gcagaggcgc gagctgctct tcaacgaggt ggtaatcatg agggactacc agcacgagaa
     3661 tgtggtggag atgtacaaca gctacctggt gggggacgag ctctgggtgg tcatggagtt
     3721 cctggaagga ggcgccctca ccgacatcgt cacccacacc aggatgaacg aggagcagat
     3781 cgcagccgtg tgccttgcag tgctgcaggc cctgtcggtg ctccacgccc agggcgtcat
     3841 ccaccgggac atcaagagcg actcgatcct gctgacccat gatggcaggg tgaagctgtc
     3901 agactttggg ttctgcgccc aggtgagcaa ggaagtgccc cgaaggaagt cgctggtcgg
     3961 cacgccctac tggatggccc cagagctcat ctcccgcctt ccctacgggc cagaggtaga
     4021 catctggtcg ctggggataa tggtgattga gatggtggac ggagagcccc cctacttcaa
     4081 cgagccaccc ctcaaagcca tgaagatgat tcgggacaac ctgccacccc gactgaagaa
     4141 cctgcacaag gtgtcgccat ccctgaaggg cttcctggac cgcctgctgg tgcgagaccc
     4201 tgcccagcgg gccacggcag ccgagctgct gaagcaccca ttcctggcca aggcagggcc
     4261 gcctgccagc atcgtgcccc tcatgcgcca gaaccgcacc agatgagaat tcgatatcaa
     4321 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4741 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     4921 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     4981 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     5041 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5101 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     5161 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5221 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5281 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     5341 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     5401 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     5461 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5521 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5581 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     5641 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5701 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5761 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5821 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5881 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6121 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6241 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6301 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6361 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6421 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6541 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6601 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6661 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6721 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     6781 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     6841 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     6901 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     6961 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7021 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7081 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7141 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7201 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7261 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7441 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7561 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7621 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7681 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7981 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8701 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9241 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9301 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9361 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9421 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9481 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9541 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9601 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
