LOCUS       sequence-350023-        6187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6187)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-ATG14-N-18
FEATURES             Location/Qualifiers
     source          1..6187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2067..2076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2073..2075,2076..2078,2079..2792)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2118..2139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2379..2398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2726..2747
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2915..3036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2952..2971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3006..3025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3043..3498)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3180..3201)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3392..3411
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3525..3629
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3631..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3631..3822
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3654..3674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4023..4817
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4023..4817
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4077..4096)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4687..4706
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5005..5024)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5049..5096
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5425..6013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5914..5933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      541 ccatggcgtc tcccagtggg aagggagccc gggcgctgga ggctcctggc tgcgggcccc
      601 ggccgctcgc ccgggacctg gtggactccg tggacgatgc ggaggggctg tacgtggctg
      661 tggagcgctg cccgctgtgc aacactaccc gccggcggct gacctgcgcc aaatgcgttc
      721 agagcggcga tttcgtctac ttcgacggcc gcgaccggga gaggtttatc gacaagaagg
      781 aaaggttaag ccgacttaag agcaagcaag aagaatttca gaaagaagtg ttaaaagcta
      841 tggaaggaaa atggataaca gatcagttga gatggaaaat aatgtcctgc aagatgagga
      901 ttgaacagtt aaaacaaaca atatgtaaag gaaatgaaga aatggagaaa aattctgaag
      961 gccttctcaa aaccaaggaa aagaatcaga agctttacag tcgagcacaa cggcaccaag
     1021 agaaaaagga gaagattcag aggcataatc gcaaacttgg tgacctggta gaaaaaaaga
     1081 ccattgactt aagaagtcat tatgagcgtc tggcaaatct tcgacgatcc catatattag
     1141 agctcacctc tgtcattttt ccaatcgagg aagtaaagac gggtgtgaga gaccccgcag
     1201 atgtgtcttc agagagtgac agtgccatga cctccagcac tgtgagcaag cttgctgaag
     1261 cccggaggac aacttacctc tcaggacgat gggtctgtga cgatcacaac ggagacacca
     1321 gcattagcat tacagggcct tggattagcc tccctaacaa tggggactac tctgcctact
     1381 acagctgggt ggaggagaag aaaacaaccc aggggcctga catggagcag agtaaccctg
     1441 cctacaccat cagtgctgcg ctgtgctatg caactcagct ggtcaacatt ctgtctcata
     1501 tacttgatgt aaatcttccc aaaaagctct gcaacagtga attttgtggc gaaaatctaa
     1561 gcaagcagaa atttactcga gcagtgaaga aactgaatgc aaatattctt tacctttgtt
     1621 tttctcagca tgtaaattta gatcaattac aaccactgca taccctcagg aatctaatgt
     1681 acctggtcag tccaagctct gaacacctag gcaggtcagg gccctttgaa gtacgagcag
     1741 accttgagga gtccatggaa tttgtggatc ccggagttgc tggagaatca gatgagagcg
     1801 gagatgagcg cgtcagcgat gaagaaaccg acctgggcac agactgggag aacttgccta
     1861 gtccccggtt ttgtgatatc ccttcccagt ctgtggaagt ctcccagagt cagagcaccc
     1921 aggcgtcccc acccatcgcg agcagcagtg caggtgggat gatctcctct gcagcagcct
     1981 cggtgacctc ctggtttaaa gcttacactg gacaccgtag cagaattctg cagtcgacgg
     2041 taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     2101 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2161 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2221 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc
     2281 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2341 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2401 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2461 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagcgaca
     2521 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     2581 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2641 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     2701 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2761 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     2821 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2881 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3001 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3061 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3121 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3181 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3241 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3301 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3361 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3421 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3481 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3601 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4021 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4081 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4141 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4201 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4261 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4321 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4381 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4441 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4501 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4561 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4621 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4681 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4741 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4801 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4861 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4921 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4981 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5041 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5101 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5161 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5221 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6121 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6181 agttccg
//
