LOCUS       sequence-350048-        9457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses voltage sensor targeted to dendrites and spines along with
            ChrimsonR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Cornejo VH, Ofer N, Yuste R
  TITLE     Voltage compartmentalization in dendritic spines in vivo.
  JOURNAL   Science. 2022 Jan 7;375(6576):82-86. doi: 10.1126/science.abg0501. 
            Epub 2021 Nov 11.
  PUBMED    34762487
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abg0501"; journalName: "Science"; date: "2022-01-7-
            7"; volume: "375"; issue: "6576"; pages: "82-86"
COMMENT     Sequence Label: pCAG-postASAP-p2a-ChrimsonR
FEATURES             Location/Qualifiers
     source          1..9457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1572..1581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2865..2921
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2928..3974
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=ChrimsonR
                     /note="K176R mutant with accelerated closing kinetics"
                     /translation="AELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEW
                     WCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIA
                     IALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYF
                     EWLLSCPVILIRLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVS
                     CIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKL
                     SPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEF
                     VEEEDEDTV"
     CDS             3987..4697
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     primer_bind     complement(4815..4832)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4821..5045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5091..5519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5178..5197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5388..5409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5528..5548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5533..5862
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5713..5848
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5775..5794
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5873..6472
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5873..5892)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6369..6389
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6632..6753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6669..6688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6723..6742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6802..6818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6802..6818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6815..6837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6826..6842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6850..6856,6857..6874,6875..6880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6895..6916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7033..7050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7204..7792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7284..7303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7963..8754,8755..8823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8586..8605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8824..8928)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8960..9339
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggtggcccct ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga
     1201 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     1261 ggctcacaaa taccactgag atccccggga ttacaatgct cgaagtcaag gaagcatcac
     1321 caaccagcat ccagatcagc tgggtgctcc acttgcgcca cgttcgctac taccgcatca
     1381 cctacggtga aactggtggc aatagccctg tccaggaatt caccgtgcct ggcagcaagt
     1441 ccactgctac catcagcggc ctgaaacctg gtgtcgacta taccatcacg gtgtacgccg
     1501 tcacgatctt cagcgcctac cgctccgcct ggccgccgat ctccatcaac taccgcaccg
     1561 gaaccctagc cgccaccatg gagacgactg tgaggtatga acaggggtca gagctcacta
     1621 aaacttcgag ctctccaaca gcagatgagc ccacgataaa gattgatgat ggtcgtgatg
     1681 agggtaatga acaagacagc tgttccaata ccattaggag aaaaatttcc ccgtttgtga
     1741 tgtcatttgg attcagagta tttggagttg tgcttatcat tgtagacatc atagtggtga
     1801 ttgtggatct ggccatcagt gagaagaaaa gaggcattag agagattctt gaaggtgttt
     1861 ccctggctat agcactcttc ttccttgttg atgttctcat gagagtgttt gttgaaggct
     1921 tcaagaacta tttccggtcc aaactgaata ctttggatgc agtcatagta gtgggcactc
     1981 tgctaattaa tatgacctac tccttctctg acggtacctt taggggcgat aacgtgtata
     2041 ttaccgcgga taaacagaaa aacggcatta aagcgaactt taccgtgcgc cataacgtgg
     2101 aagatggcag cgtgcagctg gcggatcatt atcagcagaa caccccgatt ggcgatggcc
     2161 cggtgctgct gccggataac cattatctga gcacccagac cgtgctgagc aaagatccga
     2221 acgaaaaacg cgatcacatg gtgctgctgg aatttgtgac cgcagcgggc attacacacg
     2281 gcatggatga actgtatggc ggcaccggcg gcagcgcgag ccagggcgaa gaactgttta
     2341 ccggcgtggt gccgattctg gtggaactgg atggcgatgt gaacggccat aaatttagcg
     2401 tgcgcggcga aggcgaaggc gatgcgacca ttggcaaact gaccctgaaa tttatttgca
     2461 ccaccggcaa actaccggtg ccgtggccga ccctggtgac caccttaacc tatggcgtgc
     2521 agtgctttag ccgctatccg gatcatatga aacgccatga tttttttaaa agcgcgatgc
     2581 cggaaggcta tgtgcaggaa cgcaccatta gctttaaaga tgatggcaaa tataaaaccc
     2641 gcgcggtggt gaaatttgaa ggcgataccc tggtgaaccg cattgaactg aaaggcaccg
     2701 attttaaaga agatggcaac attctggggc ataaactgga atataacaca gatcagatgc
     2761 cgcagatggt tcgtcttctt cgagttctga gaattgttat cttaataaga atatttcgcc
     2821 tggcttcaca gaagaaacaa cttgaagtgg taaccggctc cggagccacg aacttctctc
     2881 tgttaaagca agcaggagac gtggaagaaa accccggtcc cgtcgacgct gagctgatca
     2941 gcagcgccac cagatctctg tttgccgccg gaggcatcaa cccttggcct aacccctacc
     3001 accacgagga catgggctgt ggaggaatga cacctacagg cgagtgcttc agcaccgagt
     3061 ggtggtgtga cccttcttac ggactgagcg acgccggata cggatattgc ttcgtggagg
     3121 ccacaggcgg ctacctggtc gtgggagtgg agaagaagca ggcttggctg cacagcagag
     3181 gcacaccagg agaaaagatc ggcgcccagg tctgccagtg gattgctttc agcatcgcca
     3241 tcgccctgct gacattctac ggcttcagcg cctggaaggc cacttgcggt tgggaggagg
     3301 tctacgtctg ttgcgtcgag gtgctgttcg tgaccctgga gatcttcaag gagttcagca
     3361 gccccgccac agtgtacctg tctaccggca accacgccta ttgcctgcgc tacttcgagt
     3421 ggctgctgtc ttgccccgtg atcctgatca gactgagcaa cctgagcggc ctgaagaacg
     3481 actacagcaa gcggaccatg ggcctgatcg tgtcttgcgt gggaatgatc gtgttcggca
     3541 tggccgcagg actggctacc gattggctca agtggctgct gtatatcgtg tcttgcatct
     3601 acggcggcta catgtacttc caggccgcca agtgctacgt ggaagccaac cacagcgtgc
     3661 ctaaaggcca ttgccgcatg gtcgtgaagc tgatggccta cgcttacttc gcctcttggg
     3721 gcagctaccc aatcctctgg gcagtgggac cagaaggact gctgaagctg agcccttacg
     3781 ccaacagcat cggccacagc atctgcgaca tcatcgccaa ggagttttgg accttcctgg
     3841 cccaccacct gaggatcaag atccacgagc acatcctgat ccacggcgac atccggaaga
     3901 ccaccaagat ggagatcgga ggcgaggagg tggaagtgga agagttcgtg gaggaggagg
     3961 acgaggacac agtggcggca ccggtaatgg tgtctaaggg cgaagagctg atcaaggaaa
     4021 atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc aaatgcacag
     4081 gtgaaggaga aggcaatccg tacatgggaa ctcaaaccat gaggatcaaa gtcatcgagg
     4141 gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat ggcagccgta
     4201 cttttatcaa gtacccgaaa ggcattcctg atttctttaa acagtccttt cctgagggtt
     4261 ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc atgcaggaca
     4321 ccagccttga ggatggctgt ctcgtttacc acgtccaagt cagaggggta aactttccct
     4381 ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca gagatgatgt
     4441 atccagcaga tggtggtctg aggggataca ctcatatggc actgaaagtt gatggtggtg
     4501 gccatctgtc ttgctctttc gtaacaactt acaggtcaaa aaagaccgtc gggaacatca
     4561 agatgcccgg tatccatgcc gttgatcacc gcctggaaag gttagaggaa agtgacaatg
     4621 aaatgttcgt agtacaacgc gaacacgcag ttgccaagtt cgccgggctt ggtggtggga
     4681 tggacgagct gtacaagtaa tgaaagcttg gtaccgagct cggatccact agtccagtgt
     4741 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
     4801 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4861 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4921 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4981 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     5041 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     5101 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5161 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5221 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5281 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5341 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5401 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5461 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5521 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5581 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5701 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5761 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5821 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gcatgaccga
     5881 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5941 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6001 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6061 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6121 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6181 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6241 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6301 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6361 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6421 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gattcgaaat
     6481 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6541 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6601 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6661 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6721 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6781 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6901 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6961 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8941 aaaagtgcca cctgacgtcg acattgatta ttgactagtt attaatagta atcaattacg
     9001 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9061 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9121 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9181 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9241 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9301 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     9361 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     9421 taattatttt gtgcagcgat gggggcgggg ggggggg
//
