LOCUS       sequence-350108-       15126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PE2-NG variant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15126)
  AUTHORS   Jang H, Jo DH, Cho CS, Shin JH, Seo JH, Yu G, Gopalappa R, Kim D, 
            Cho SR, Kim JH, Kim HH
  TITLE     Application of prime editing to the correction of mutations and 
            phenotypes in adult mice with liver and eye diseases.
  JOURNAL   Nat Biomed Eng. 2021 Aug 26. pii: 10.1038/s41551-021-00788-9. doi: 
            10.1038/s41551-021-00788-9.
  PUBMED    34446856
REFERENCE   2  (bases 1 to 15126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2021 Aug 26. pii: 10.1038/s41551-021-00788-9. doi: 
            10.1038/s41551-021-00788-9."
COMMENT     Sequence Label: pLenti-NG-PE2-BSD
FEATURES             Location/Qualifiers
     source          1..15126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(624..1415,1416..1484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1247..1266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1485..1589)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1664..1683)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1855..2234
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2236..2434
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2389..2409
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2452..2632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2679..2804
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3297..3530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3715..3759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4057..4174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4267..4478
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4534..4554
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4555..8655
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10828..10848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10858..10914
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10915..11313
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11456..12044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11509..11529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11927..11938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12047..12063)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12048..12064)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             12569..12749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12775..12792)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12781..13005
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13051..13479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13138..13157)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13348..13369
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13488..13508)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      13653..13787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13714..13733
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13849..13896
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13915..14289
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14419..14540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14456..14475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14510..14529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14589..14605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14589..14605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14602..14624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14613..14629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14637..14643,14644..14661,14662..14667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14682..14703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14820..14837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(14991..15126,1..453))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15071..15090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
       61 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      181 aacccggtaa gacacgactt atcgccactt gcagcagcca ctggtaacag gattagcaga
      241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      301 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     1621 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     1681 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     1741 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     1801 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     1861 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     1921 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     1981 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2041 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2101 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2161 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2221 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2281 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2341 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2401 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     2461 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2521 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2581 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2641 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2701 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2761 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2821 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2881 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2941 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     3001 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     3061 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     3121 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     3181 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     3241 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     3301 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     3361 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     3421 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     3481 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     3541 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3601 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3661 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3721 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3781 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3841 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     3901 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     3961 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     4021 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     4081 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     4141 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     4201 tccagtttgg ttaattagct agctaggtct tgaaaggagt gggaattggc tccggtgccc
     4261 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     4321 attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     4381 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     4441 acgttctttt tcgcaacggg tttgccgcca gaacacagga ccggttctag agccaccatg
     4501 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcgacaag
     4561 aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac
     4621 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
     4681 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
     4741 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
     4801 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
     4861 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     4921 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     4981 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     5041 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     5101 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     5161 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
     5221 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc
     5281 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     5341 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     5401 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     5461 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag
     5521 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     5581 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt
     5641 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     5701 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     5761 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     5821 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     5881 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     5941 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     6001 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     6061 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag
     6121 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     6181 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     6241 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     6301 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     6361 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     6421 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     6481 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     6541 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     6601 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     6661 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     6721 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     6781 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     6841 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     6901 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     6961 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     7021 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct
     7081 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     7141 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     7201 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     7261 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     7321 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     7381 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     7441 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     7501 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     7561 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     7621 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     7681 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     7741 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     7801 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     7861 ggcttcagca aagagtctat caggcccaag aggaacagcg ataagctgat cgccagaaag
     7921 aaggactggg accctaagaa gtacggcggc ttcgtcagcc ccaccgtggc ctattctgtg
     7981 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     8041 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     8101 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     8161 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccagattcct gcagaaggga
     8221 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     8281 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag
     8341 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     8401 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     8461 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctagggcc
     8521 ttcaagtact ttgacaccac catcgaccgg aaggtgtaca ggagcaccaa agaggtgctg
     8581 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     8641 cagctgggag gcgactctgg aggatctagc ggaggatcct ctggcagcga gacaccagga
     8701 acaagcgagt cagcaacacc agagagcagt ggcggcagca gcggcggcag cagcacccta
     8761 aatatagaag atgagtatcg gctacatgag acctcaaaag agccagatgt ttctctaggg
     8821 tccacatggc tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat gggactggca
     8881 gttcgccaag ctcctctgat catacctctg aaagcaacct ctacccccgt gtccataaaa
     8941 caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca gagactgttg
     9001 gaccagggaa tactggtacc ctgccagtcc ccctggaaca cgcccctgct acccgttaag
     9061 aaaccaggga ctaatgatta taggcctgtc caggatctga gagaagtcaa caagcgggtg
     9121 gaagacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct cccaccgtcc
     9181 caccagtggt acactgtgct tgatttaaag gatgcctttt tctgcctgag actccacccc
     9241 accagtcagc ctctcttcgc ctttgagtgg agagatccag agatgggaat ctcaggacaa
     9301 ttgacctgga ccagactccc acagggtttc aaaaacagtc ccaccctgtt taatgaggca
     9361 ctgcacagag acctagcaga cttccggatc cagcacccag acttgatcct gctacagtac
     9421 gtggatgact tactgctggc cgccacttct gagctagact gccaacaagg tactcgggcc
     9481 ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc
     9541 cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg gctgactgag
     9601 gccagaaaag agactgtgat ggggcagcct actccgaaga cccctcgaca actaagggag
     9661 ttcctaggga aggcaggctt ctgtcgcctc ttcatccctg ggtttgcaga aatggcagcc
     9721 cccctgtacc ctctcaccaa accggggact ctgtttaatt ggggcccaga ccaacaaaag
     9781 gcctatcaag aaatcaagca agctcttcta actgccccag ccctggggtt gccagatttg
     9841 actaagccct ttgaactctt tgtcgacgag aagcagggct acgccaaagg tgtcctaacg
     9901 caaaaactgg gaccttggcg tcggccggtg gcctacctgt ccaaaaagct agacccagta
     9961 gcagctgggt ggcccccttg cctacggatg gtagcagcca ttgccgtact gacaaaggat
    10021 gcaggcaagc taaccatggg acagccacta gtcattctgg ccccccatgc agtagaggca
    10081 ctagtcaaac aaccccccga ccgctggctt tccaacgccc ggatgactca ctatcaggcc
    10141 ttgcttttgg acacggaccg ggtccagttc ggaccggtgg tagccctgaa cccggctacg
    10201 ctgctcccac tgcctgagga agggctgcaa cacaactgcc ttgatatcct ggccgaagcc
    10261 cacggaaccc gacccgacct aacggaccag ccgctcccag acgccgacca cacctggtac
    10321 acggatggaa gcagtctctt acaagaggga cagcgtaagg cgggagctgc ggtgaccacc
    10381 gagaccgagg taatctgggc taaagccctg ccagccggga catccgctca gcgggctgaa
    10441 ctgatagcac tcacccaggc cctaaagatg gcagaaggta agaagctaaa tgtttatact
    10501 gatagccgtt atgcttttgc tactgcccat atccatggag aaatatacag aaggcgtggg
    10561 tggctcacat cagaaggcaa agagatcaaa aataaagacg agatcttggc cctactaaaa
    10621 gccctctttc tgcccaaaag acttagcata atccattgtc caggacatca aaagggacac
    10681 agcgccgagg ctagaggcaa ccggatggct gaccaagcgg cccgaaaggc agccatcaca
    10741 gagactccag acacctctac cctcctcata gaaaattcat caccctctgg cggctcaaaa
    10801 agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcgg atccggcgca
    10861 acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg accgatggcc
    10921 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
    10981 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
    11041 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
    11101 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
    11161 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
    11221 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
    11281 ttgctgccct ctggttatgt gtgggagggc taatgtacaa gtaagcggcc gcgactctag
    11341 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    11401 ctccccctga acctgaaaca taaaatgaat gcaattcgat atcaagctta tcggtaatca
    11461 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    11521 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    11581 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    11641 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    11701 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    11761 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    11821 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
    11881 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    11941 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    12001 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
    12061 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
    12121 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
    12181 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    12241 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
    12301 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
    12361 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
    12421 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
    12481 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
    12541 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
    12601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    12661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    12721 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
    12781 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    12841 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    12901 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    12961 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    13021 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    13081 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    13141 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    13201 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    13261 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    13321 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    13381 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    13441 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    13501 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    13561 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    13621 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccagttccgc
    13681 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    13741 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    13801 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
    13861 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
    13921 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
    13981 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
    14041 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
    14101 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
    14161 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
    14221 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
    14281 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    14341 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    14401 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    14461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    14521 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    14581 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    14641 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    14701 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    14761 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    14821 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    14881 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    14941 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    15001 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    15061 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    15121 ccgacc
//
