LOCUS       sequence-350169-       10075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of nuclear-localized dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Hung ME, Leonard JN
  TITLE     A platform for actively loading cargo RNA to elucidate limiting 
            steps in EV-mediated delivery.
  JOURNAL   J Extracell Vesicles. 2016 May 13;5:31027. doi: 
            10.3402/jev.v5.31027. eCollection 2016.
  PUBMED    27189348
REFERENCE   2  (bases 1 to 10075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Extracell
            Vesicles."; date: "2016-05-13"; volume: "5:31027. doi"; pages: " 
            10.3402/jev.v5.31027. eCollection 2016"
COMMENT     Sequence Label: pMEH275 NLS dTomato
FEATURES             Location/Qualifiers
     source          1..10075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             140..773
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    820..945
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1442..1675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1860..1904
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2202..2319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2384..2758)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2755..2764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2768..2786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2778..2825)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2930..3233
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3234..3437
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3388..3408
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3434..3458
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3568..3588
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3598..4299
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3731..3749)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3999..4018
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4193..4216
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4309..4897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4362..4382)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4780..4791)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4984..5217
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5318..5335)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5324..5548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5594..6022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5681..5700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5891..5912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6031..6051)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6036..6365
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6216..6351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6278..6297
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6414..7439
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6414..7439
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7569..7690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7739..7755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7739..7755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7752..7774)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7763..7779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7787..7793,7794..7811,7812..7817))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7832..7853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7970..7987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8141..8729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8221..8240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8900..9691,9692..9760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9523..9542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9761..9865)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9913..10047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
       61 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
      121 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
      181 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
      241 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
      301 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
      361 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
      421 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
      481 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
      541 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
      601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      781 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      841 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      901 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      961 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1021 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1081 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1141 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1201 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1261 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     1321 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1381 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1441 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1501 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1561 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1621 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1681 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1741 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1801 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1861 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1921 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1981 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2041 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2101 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2161 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2221 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2281 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2341 agagatccag tttggttagt accgggcccg ctctagtccg gaatcagtcc tgctcctcgg
     2401 ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct
     2461 gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct
     2521 ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt
     2581 ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac
     2641 cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga
     2701 ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg
     2761 tggccctcct atagtgagtc gtattatact atgccgatat actatgccga tgattaattg
     2821 tcaacacgtg ctgcaggtcc gaggttctag acgtattacc gccatgcatt agttattaat
     2881 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     2941 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3001 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3061 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3121 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3181 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     3241 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     3301 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     3361 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     3421 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     3481 gttttgacct ccatagaaga caccgactct actagaggat ctgccaccag ctagcagaga
     3541 ttcgctccaa ggtgtaccgc cgccatgccc aagaaaaagc ggaaggtgtc ccggggaatg
     3601 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     3661 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     3721 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     3781 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     3841 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     3901 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     3961 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     4021 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     4081 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     4141 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     4201 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     4261 caccacctgt tcctgtacgg catggacgag ctgtacaagt aggtcgacaa tcaacctctg
     4321 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4381 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4441 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4501 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4561 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4621 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4681 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4741 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4801 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4861 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     4921 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     4981 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5041 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5101 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5161 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5221 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5281 gtgagaggcc ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5341 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5401 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5461 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5521 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5581 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5701 tcctttctcg tcacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6001 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6061 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6121 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6181 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6241 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6301 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6361 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa
     6421 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     6481 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     6541 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     6601 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     6661 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     6721 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     6781 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     6841 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     6901 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     6961 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     7021 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     7081 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     7141 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     7201 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     7261 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     7321 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     7381 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     7441 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7501 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7561 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7621 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7681 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9901 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10021 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatc
//
