LOCUS       sequence-350207-       14547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, 
            Karton A, Piggott AM, Chooi YH
  TITLE     Heterologous biosynthesis of elsinochrome A sheds light on the 
            formation of the photosensitive perylenequinone system.
  JOURNAL   Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. 
            eCollection 2019 Feb 7.
  PUBMED    30809363
REFERENCE   2  (bases 1 to 14547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c8sc02870b"; journalName: "Chem Sci"; date: "2018-11-22- 
            22"; volume: "10"; issue: "5"; pages: "1457-1465"
COMMENT     Sequence Label: pYFAC-pyroA
FEATURES             Location/Qualifiers
     source          1..14547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(6631..6699,6700..7491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6849..6868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7662..8250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8151..8170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8404..8421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8538..8559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8574..8579,8580..8597,8598..8604)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8612..8628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8617..8639
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8636..8652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8636..8652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      8669..10011
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9632..9679)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10052..10070
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10108..10130)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10230..10249
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10273..10488
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10489..11292
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(11574..11591)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11574..11590)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11583..11605)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11890..11909)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      join(11931..14547,1..2632)
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgcggccg ccccctggta tcatggttgt tgggtctcat cactcctact
     3421 caacattgca cctgataggg acatcagatg ctggattact aaggtaatgt aaggtcagtt
     3481 cgagaccatc tgatattacc acaaatacaa tggcgagaga gtttttcgta aaagccaatc
     3541 cttggcgttt ccagctgttc ctgacggttg taggcccaag tccgcgggaa accgcccaca
     3601 aagcggcgtt tttgcagatt ggcagattta tgctggaaac ttactgggga gatggagggg
     3661 cacaagcgct gtgattggtt ttcaaagccg cggccggatg gaacgaagac ataattcggc
     3721 ggggacatga aaatgtgggt gatcgatacg gaatttttgg ttcttcggag gcgacaaagg
     3781 gcgcaacggt cgaggttagt agttatcttg actcacactt acagggcccg tcttcggtct
     3841 tcttaagaac tgggttttgc tgggacttcc cccccacctc tcttttctac tgtgtctcgt
     3901 atctatttct atactcattc tttcacttct cttagtacca ccattccctt ctaaatacac
     3961 agaatggctt ccaacggtac caatggcgcc tccgcctcca acagcttcac tgtgaaggcc
     4021 ggcttggctc agatgctgaa gggtggtgtg attatggacg tcgtcaacgc ggagcaggta
     4081 tgagcgattg tcatcaggat acttccagcc ctttgacgct aacatgactt ctacaacagg
     4141 cccgcattgc ggaggaggcc ggtgccgctg ccgtgatggc cctggagaga gtccccgccg
     4201 acatcagagc ccagggtggc gttgcccgca tgtctgaccc cagcatgatc aaggagatca
     4261 tggctgctgt taccattcct gtcatggcca aagctcgtat cggacacttc gttgagtgcc
     4321 aggtaaggct gcctttctcc cgtggaaagc ctgcattgca gctaacatgt gtaattgtta
     4381 gatcctcgaa gccattggcg ttgactacat cgacgagtcc gaagtcctta cccctgccga
     4441 tgatgtctac cacgtgaaga agcacgacta caaggttcct ttcgtctgtg gttgccgcaa
     4501 cctgggcgag gcccttcgtc ggatcgccga gggtgccgct atgatccgta ccaagggtga
     4561 ggccggtacc ggagatgttg ttgaagccgt caagcacatg cgcacggtca actcccagat
     4621 cgcccgcgcc cgctccatcc tccagaattc caccgacccc gagattgagc tgcgtgccta
     4681 cgctcgtgag cttgaggtcc cttatgagct tctgcgcgag accgccgaga agggccgtct
     4741 tcccgttgtc aacttcgccg ccggcggtgt tgccactccc gctgatgccg cactcatgat
     4801 gcagctgggc tgcgacggag tgttcgtcgg ctctggtatt ttcaagtctg gtgatgcgaa
     4861 gaagcgcgcc aaggctattg tccaggccgt gactcactac aaggacccca aggtcctcgc
     4921 tgaagtcagc gagggtctgg gtgaggccat ggttggtatc aatgtctctc agatgcccga
     4981 ggccgaccga ttggccaaga gaggatggta attgcactac tatctctact tgtgattctt
     5041 cttatgttct tgtcatgata tgggcgttgg aaaagttgat atagcgttct ttgatgcatt
     5101 ttgcattcaa gactttcagg ttcattcttg ttagggtgtt ctgtgcattt gtccttcatt
     5161 atgtagacac tcgcaggatt gaagaagaac tactctattc aatgacgatc tcttccatga
     5221 agaccaaaaa ctaaaaattg gtcatgtcaa cgtccagtgc acacgacatt ttacccttcc
     5281 ccactaacac ttgttttggc tgtcgatcat gtggatgctg ctactggtgg tgctaatctg
     5341 cttgaagacg cggcgagtag tatcaccaag taatatactt atattgcgaa gataatttat
     5401 atctgatatt tcaattacaa attctgagac cactacccag ttgacggcac tcatgctggg
     5461 tgccttagat agacgtcaag gtacacatcc tacggagtta cgatcggcga cttcaaaaga
     5521 atgtcacacc ggtaagatac acacgtttat ttcaaacgcc gaacttgaat acacaccctg
     5581 acagcgccat aagacaattt cctcttggag caaaagtgta gtgccagtac gagtgttgtg
     5641 gaggaaggct gcatacattg tgcctgtcat taaacgatga gctcgtccgt attggcccct
     5701 gtaatgccat gttttccgcc cccaatcgtc aaggttttcc ctttgttaga ttcctaccag
     5761 tcatctagca agtgaggtaa gctttgccag aaacgccaag gctttatcta tgtagtcgat
     5821 aagcaaagtg gactgatagc ttaatatgga aggtccctca ggacaagtcg acctgtgcag
     5881 aagagataac agcttggcat cacgcatcag tgcctcctct cagacagaat aagtcgccat
     5941 aagttatcga ccgaacctag gtagggtata tttggtgacg acaatacctc ccgacgatac
     6001 ctggattaga caccgtatgc cgcgaattaa cgccttccga ccaagtccct tttggtgatc
     6061 gacactttag gatcatctcc tgccagtcgc ccagactgac ggtgttatgg tggcgcgcta
     6121 tggctttcct agccggtcaa gctccgtgca ctagagagcg gagaaggagg gggagttgat
     6181 agggaatgat atctaggtag agaagtactc caaccagaga tatgcggatc aatgctccgg
     6241 cagttgttag cataccccaa cctcgtttaa gtatcctctt cttccatcca tctttatcat
     6301 tatttgtcta tgtgcatccc tagatcacgt caattatttc gataaatgga tacgtgcact
     6361 cgggaatgcg tgattggctc gtctcgtgtt ctcagttcct cattcccccc cacgttacat
     6421 tgacaacgga gtattagcct atggtcctgt tcgataagct tgctccatcg cctccactac
     6481 gtcaagcgga gtatcagaca tgagctctac cccagatatc gcctgcttat cgctggttgc
     6541 tttgtcatgc aagtacagat cacatcggca attctgataa cagatggtgc cattgtcccg
     6601 taatcaattg cccattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6661 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6721 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6781 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6841 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6901 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6961 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7021 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7081 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7141 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7201 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7261 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7321 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7381 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7441 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7501 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7561 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7621 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7681 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7741 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7801 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7861 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7921 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7981 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8041 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8101 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8161 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8221 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8281 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8341 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8401 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8461 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8521 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8581 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8641 aacagctatg accatgatta cgccactgaa cgaagcatct gtgcttcatt ttgtagaaca
     8701 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     8761 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     8821 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     8881 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     8941 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     9001 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     9061 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     9121 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     9181 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     9241 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     9301 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     9361 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     9421 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     9481 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     9541 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     9601 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga
     9661 acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg
     9721 agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat
     9781 atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg
     9841 tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc
     9901 ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc
     9961 aattggatta gtctcatcct tcaatgctat catttccttt gatattggat catactaaga
    10021 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10081 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10141 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10201 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10261 ccataccacc ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct
    10321 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
    10381 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
    10441 ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct
    10501 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
    10561 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
    10621 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
    10681 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
    10741 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
    10801 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
    10861 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
    10921 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
    10981 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
    11041 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
    11101 ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc
    11161 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
    11221 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
    11281 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
    11341 gcttcaattt aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt
    11401 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
    11461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    11521 aaatcaaaag aatagaccga gatagggttg agtggggtac cgagctcgaa ttcactggcc
    11581 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    11641 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    11701 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
    11761 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
    11821 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
    11881 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc agcttatttt
    11941 ttgtatactg ttttgtgata gcacgaagtt tttccacggt atcttgttaa aaatatatat
    12001 ttgtggcggg cttacctaca tcaaattaat aagagactaa ttataaacta aacacacaag
    12061 caagctactt tagggtaaaa gtttataaat gcttttgacg tataaacgtt gcttgtattt
    12121 attattacaa ttaaaggtgg atagaaaacc tagagactag ttagaaacta atctcaggtt
    12181 tgcgttaaac taaatcagag cccgagaggt taacagaacc tagaagggga ctagatatcc
    12241 gggtagggaa acaaaaaaaa aaaacaagac agccacatat tagggagact agttagaagc
    12301 tagttccagg actaggaaaa taaaagacaa tgataccaca gtctagttga caactagata
    12361 gattctagat tgaggccaaa gtctctgaga tccaggttag ttgcaactaa tactagttag
    12421 tatctagtct cctataactc tgaagctaga ataacttact actattatcc tcaccactgt
    12481 tcagctgcgc aaacggagtg attgcaaggt gttcagagac tagttattga ctagtcagtg
    12541 actagcaata actaacaagg tattaaccta ccatgtctgc catcaccctg cacttcctcg
    12601 ggctcagcag ccttttcctc ctcattttca tgctcatttt ccttgtttaa gactgtgact
    12661 agtcaaagac tagtccagaa ccacaaagga gaaatgtctt accactttct tcattgcttg
    12721 tctcttttgc attatccatg tctgcaacta gttagagtct agttagtgac tagtccgacg
    12781 aggacttgct tgtctccgga ttgttggagg aactctccag ggcctcaaga tccacaacag
    12841 agccttctag aagactggtc aataactagt tggtctttgt ctgagtctga cttacgaggt
    12901 tgcatactcg ctccctttgc ctcgtcaatc gatgagaaaa agcgccaaaa ctcgcaatat
    12961 ggctttgaac cacacggtgc tgagactagt tagaatctag tcccaaacta gcttggatag
    13021 cttacctttg ccctttgcgt tgcgacaggt cttgcagggt atggttcctt tctcaccagc
    13081 tgatttagct gccttgctac cctcacggcg gatctgcata aagagtggct agaggttata
    13141 aattagcact gatcctaggt acggggctga atgtaacttg cctttccttt ctcatcgcgc
    13201 ggcaagacag gcttgctcaa attcctacca gtcacagggg tatgcacggc gtacggacca
    13261 cttgaactag tcacagatta gttagcaact agtctgcatt gaatggctgt acttacgggc
    13321 cctcgccatt gtcctgatca tttccagctt caccctcgtt gctgcaaagt agttagtgac
    13381 tagtcaagga ctagttgaaa tgggagaaga aactcacgaa ttctcgacac ccttagtatt
    13441 gtggtccttg gacttggtgc tgctatatat tagctaatac actagttaga ctcacagaaa
    13501 cttacgcagc tcgcttgcgc ttcttggtag gagtcggggt tgggagaaca gtgccttcaa
    13561 acaagccttc ataccatgct acttgactag tcagggacta gtcaccaagt aatctagata
    13621 ggacttgcct ttggcctcca tcagttcctt catagtggga ggtccattgt gcaatgtaaa
    13681 ctccatgccg tgggagttct tgtccttcaa gtgcttgacc aatatgtttc tgttggcaga
    13741 gggaacctgt caactagtta ataactagtc agaaactagt atagcagtag actcactgta
    13801 cgcttgaggc atcccttcac tcggcagtag acttcatatg gatggatatc aggcacgcca
    13861 ttgtcgtcct gtggactagt cagtaactag gcttaaagct agtcgggtcg gcttactatc
    13921 ttgaaatccg gcagcgtaag ctccccgtcc ttaactgcct cgagatagtg acagtactct
    13981 ggggactttc ggagatcgtt atcgcgaatg ctcggcatac taatcgttga ctagtcttgg
    14041 actagtcccg agcaaaaagg attggaggag gaggaggaag gtgagagtga gacaaagagc
    14101 gaaataagag cttcaaaggc tatctctaag cagtatgaag gttaagtatc tagttcttga
    14161 ctagatttaa aagagatttc gactagttat gtacctggag tttggatata ggaatgtgtt
    14221 gtggtaacga aatgtaaggg ggaggaaaga aaaagtcggt caagaggtaa ctctaagtcg
    14281 gccattcctt tttgggaggc gctaaccata aacggcatgg tcgacttaga gttagctcag
    14341 ggaatttagg gagttatctg cgaccactgg actctcaggc tttccattgg ggtactaaca
    14401 tagccatcaa atgccggccc gtgtttaatc tcctcgagat ggctgggctc acatgcgttc
    14461 tactcaaaaa ctcttatacc taggccctct cccaatcgtg tatgtttgga ccttcctctc
    14521 tcacactttg gataacatca agcatac
//
