LOCUS       sequence-350216-        5440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Lymnaea stagnalis AChBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Celie PH, Kasheverov IE, Mordvintsev DY, Hogg RC, van Nierop P, van 
            Elk R, van Rossum-Fikkert SE, Zhmak MN, Bertrand D, Tsetlin V, Sixma
            TK, Smit AB
  TITLE     Crystal structure of nicotinic acetylcholine receptor homolog AChBP 
            in complex with an alpha-conotoxin PnIA variant.
  JOURNAL   Nat Struct Mol Biol. 2005 Jul;12(7):582-8. Epub 2005 Jun 12.
  PUBMED    15951818
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2005-07"; volume: "12(7)"; pages: "582-8. Epub 
            2005 Jun 12"
COMMENT     Sequence Label: pFastBac1-Ls-AChBP
FEATURES             Location/Qualifiers
     source          1..5440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(995..1000,1001..1017,1018..1023))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1375..1466
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1391..1408
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    2420..2554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2457..2476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2511..2530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2583..2748
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2932..3386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3019..3038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3228..3249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3412..3516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3517..3585,3586..4377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3735..3754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4548..5136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5037..5056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5290..5307
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      841 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      901 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      961 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     1021 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     1081 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     1141 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     1201 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     1261 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     1321 ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg
     1381 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1441 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1501 gcggatccgc caccatgcgt cgaaacattt tctgccttgc ttgtctctgg atcgtgcaag
     1561 cgtgtctaag cttggaccgg gcagacatct tgtacaacat acgtcagaca tcgagaccgg
     1621 atgtgattcc cacacagcga gatcgcccag tggcggtgtc cgtctctttg aagttcatca
     1681 acatcttgga agtgaatgaa ataaccaatg aagtggacgt ggtcttttgg cagcagacga
     1741 catggtcgga caggaccctc gcctggaaca gttctcactc accagatcag gtttccgtgc
     1801 caataagctc tttgtgggtg cctgacctcg ctgcatacaa cgccatctcg aaacctgaag
     1861 tccttacacc gcaactggcc agggtcgtat ccgatggtga agtgctgtac atgccgagta
     1921 tccgccagcg gttctcctgc gatgtatcgg gtgtcgatac ggagtccggt gctacatgtc
     1981 ggatcaaaat tggttcctgg acccaccaca gtagagagat ttctgtagat cccacgacag
     2041 aaaatagtga tgattctgaa tacttctccc aatactctcg ctttgaaatc ttggacgtca
     2101 cacagaagaa gaactcggtt acctactctt gctgtccgga ggcatacgag gacgttgaag
     2161 tgagtctcaa tttccggaag aagggacgct ccgaaattct ttagtaagaa ttcaaaggcc
     2221 tacgtcgacg agctcactag tcgcggccgc tttcgaatct agagcctgca gtctcgaggc
     2281 atgcggtacc aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
     2341 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2401 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2461 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2521 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc
     2581 cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
     2641 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
     2701 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
     2761 tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt
     2821 cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt
     2881 ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct
     2941 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3001 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3061 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3121 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3181 gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt
     3241 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3301 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3361 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     3421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4381 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4681 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5221 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5281 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     5341 cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg
     5401 gttacggttg agtaataaat ggatgccctg cgtaagcggg
//
