LOCUS       sequence-35030-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Erk2
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctctagaaat aattttgttt aactttaaga aggagatata catatgcacc atcatcatca
       61 tcattcttct ggtgtagatc tgggtaccga gaacctgtac ttccaatcca atgcggcggc
      121 ggcggcggcg ggcccggaga tggtccgcgg gcaggtgttc gacgtggggc cgcgctacac
      181 taatctctcg tacatcggag aaggcgccta cggcatggtt tgttctgctt atgataatct
      241 caacaaagtt cgagttgcta tcaagaaaat cagtcctttt gagcaccaga cctactgtca
      301 gagaaccctg agagagataa aaatcctact gcgcttcaga catgagaaca tcatcggcat
      361 caatgacatc atccgggcac caaccattga gcagatgaaa gatgtatata tagtacagga
      421 cctcatggag acagatcttt acaagctctt gaagacacag cacctcagca atgatcatat
      481 ctgctatttt ctttatcaga tcctgagagg attaaagtat atacattcag ctaatgttct
      541 gcaccgtgac ctcaagcctt ccaacctcct gctgaacacc acttgtgatc tcaagatctg
      601 tgactttggc cttgcccgtg ttgcagatcc agaccatgat catacagggt tcttgacaga
      661 gtatgtagcc acgcgttggt acagagctcc agaaattatg ttgaattcca agggttatac
      721 caagtccatt gatatttggt ctgtgggctg catcctggca gagatgctat ccaacaggcc
      781 tatcttccca ggaaagcatt accttgacca gctgaatcac atcctgggta ttcttggatc
      841 tccatcacag gaagatctga attgtataat aaatttaaaa gctagaa
//
