LOCUS       sequence-350557-       11070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Delivers all prime editing nuclease components in a single plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Adikusuma F, Lushington C, Arudkumar J, Godahewa GI, Chey YCJ, 
            Gierus L, Piltz S, Geiger A, Jain Y, Reti D, Wilson LOW, Bauer DC, 
            Thomas PQ
  TITLE     Optimized nickase- and nuclease-based prime editing in human and 
            mouse cells.
  JOURNAL   Nucleic Acids Res. 2021 Sep 17. pii: 6371974. doi: 
            10.1093/nar/gkab792.
  PUBMED    34534334
REFERENCE   2  (bases 1 to 11070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Sep 17. pii: 6371974. doi: 10.1093/nar/gkab792."
COMMENT     Sequence Label: PEA1-Nuclease
FEATURES             Location/Qualifiers
     source          1..11070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          173..400
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      412..421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             421..486
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             493..513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             538..4638
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             538..4638
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6811..6858
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6886..6903)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6892..7099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7108..7124
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7108..7124
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_RNA        complement(7167..7242)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7260..7508)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     repeat_region   7517..7657
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7517..7646
                     /label=AAV2 ITR
     rep_origin      7732..8187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7819..7838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8029..8050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8204..8223)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8323..8345
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8383..8401)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8469..8573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8574..8642,8643..9434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8792..8811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9605..10193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10094..10113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10255..10503
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        10522..10597
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        10682..10967
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(10969..11070,1..172)
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggcgggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
       61 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
      121 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      181 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      241 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      301 taattagctg agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt
      361 taattacctg gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg
      421 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
      481 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac
      541 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
      601 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
      661 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
      721 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
      781 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
      841 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
      901 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
      961 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
     1021 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
     1081 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
     1141 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
     1201 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
     1261 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
     1321 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
     1381 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
     1441 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
     1501 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
     1561 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
     1621 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
     1681 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
     1741 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
     1801 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
     1861 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
     1921 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
     1981 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
     2041 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
     2101 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
     2161 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
     2221 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
     2281 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
     2341 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
     2401 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
     2461 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
     2521 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
     2581 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
     2641 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
     2701 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
     2761 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
     2821 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
     2881 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
     2941 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
     3001 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg
     3061 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
     3121 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
     3181 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
     3241 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
     3301 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
     3361 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
     3421 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
     3481 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
     3541 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
     3601 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
     3661 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
     3721 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
     3781 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
     3841 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
     3901 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
     3961 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
     4021 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
     4081 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
     4141 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
     4201 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
     4261 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
     4321 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
     4381 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
     4441 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
     4501 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac gaaagaggtg
     4561 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
     4621 tctcagctgg gaggtgactc tggaggatct agcggaggat cctctggcag cgagacacca
     4681 ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg cagcagcacc
     4741 ctaaatatag aagatgagta tcggctacat gagacctcaa aagagccaga tgtttctcta
     4801 gggtccacat ggctgtctga ttttcctcag gcctgggcgg aaaccggggg catgggactg
     4861 gcagttcgcc aagctcctct gatcatacct ctgaaagcaa cctctacccc cgtgtccata
     4921 aaacaatacc ccatgtcaca agaagccaga ctggggatca agccccacat acagagactg
     4981 ttggaccagg gaatactggt accctgccag tccccctgga acacgcccct gctacccgtt
     5041 aagaaaccag ggactaatga ttataggcct gtccaggatc tgagagaagt caacaagcgg
     5101 gtggaggaca tccaccccac cgtgcccaac ccttacaacc tcttgagcgg gctcccaccg
     5161 tcccaccagt ggtacactgt gcttgattta aaggatgcct ttttctgcct gagactccac
     5221 cccaccagtc agcctctctt cgcctttgag tggagagatc cagagatggg aatctcagga
     5281 caattgacct ggaccagact cccacagggt ttcaaaaaca gtcccaccct gtttaatgag
     5341 gcactgcaca gagacctagc agacttccgg atccagcacc cagacttgat cctgctacag
     5401 tacgtggatg acttactgct ggccgccact tctgagctag actgccaaca aggtactcgg
     5461 gccctgttac aaaccctagg gaacctcggg tatcgggcct cggccaagaa agcccaaatt
     5521 tgccagaaac aggtcaagta tctggggtat cttctaaaag agggtcagag atggctgact
     5581 gaggccagaa aagagactgt gatggggcag cctactccaa agacccctcg acaactaagg
     5641 gagttcctag ggaaggcagg cttctgtcgc ctcttcatcc ctgggtttgc agaaatggca
     5701 gcccccctgt accctctcac caaaccgggg actctgttta attggggccc agaccaacaa
     5761 aaggcctatc aagaaatcaa gcaagccctt ctaactgccc cagccctggg gttgccagat
     5821 ttgactaagc cctttgaact ctttgtcgac gagaagcagg gctacgccaa aggtgtccta
     5881 acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc tgtccaaaaa gctagaccca
     5941 gtagcagctg ggtggccccc ttgcctacgg atggtagcag ccattgccgt actgacaaag
     6001 gatgcaggca agctaaccat gggacagcca ctagtcattc tggcccccca tgcagtagag
     6061 gcactagtca aacaaccccc cgaccgctgg ctttccaacg cccggatgac tcactatcag
     6121 gccttgcttt tggacacgga ccgggtccag ttcggaccgg tggtagccct gaacccggct
     6181 acgctgctcc cactgcctga ggaagggctg caacacaact gccttgatat cctggccgaa
     6241 gcccacggaa cccgacccga cctaacggac cagccgctcc cagacgccga ccacacctgg
     6301 tacacggatg gaagcagtct cttacaagag ggacagcgta aggcgggagc tgcggtgacc
     6361 accgagaccg aggtaatctg ggctaaagcc ctgccagccg ggacatccgc tcagcgggct
     6421 gaactgatag cactcaccca ggccctaaag atggcagaag gtaagaagct aaatgtttat
     6481 actgatagcc gttatgcttt tgctactgcc catatccatg gagaaatata cagaaggcgt
     6541 gggtggctca catcagaagg caaagagatc aaaaataaag acgagatctt ggccctacta
     6601 aaagccctct ttctgcccaa aagacttagc ataatccatt gtccaggaca tcaaaaggga
     6661 cacagcgccg aggctagagg caaccggatg gctgaccaag cggcccgaaa ggcagccatc
     6721 acagagactc cagacacctc taccctcctc atagaaaatt catcaccctc tggcggctca
     6781 aaaagaaccg ccgacggcag cgaattcgag aaaaggccgg cggccacgaa aaaggccggc
     6841 caggcaaaaa agaaaaagta agaattccta gagctcgctg atcagcctcg actgtgcctt
     6901 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6961 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     7021 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga
     7081 atagcaggca tgctggggag cggccgctct agaactagtg gatcccccgg gctgcaggaa
     7141 ttcgatacga atcctgcagg aaaaaagcac cgactcggtg ccactttttc aagttgataa
     7201 cggactagcc ttattttaac ttgctatttc tagctctaaa acgtcttctc gaagaccccg
     7261 gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg
     7321 gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat
     7381 tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt
     7441 ccaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat
     7501 aggccctcgc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     7561 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     7621 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     7681 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     7741 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7801 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7861 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7921 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     7981 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8041 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     8101 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8161 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     8221 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8281 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8341 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     8401 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8461 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8521 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8581 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8641 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8701 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8761 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8821 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8881 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8941 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9001 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9061 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9121 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9181 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     9301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9661 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9721 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9781 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9841 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9901 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9961 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10021 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10081 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10141 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10201 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtgagggc
    10261 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
    10321 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
    10381 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
    10441 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
    10501 accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa taaggctagt
    10561 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcggg tcttcgagaa gacctttttt
    10621 ttgttctagt ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac
    10681 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
    10741 tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    10801 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    10861 acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact
    10921 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
    10981 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
    11041 ttatttttta attattttgt gcagcgatgg
//
