LOCUS       sequence-350834-       10719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flp recombinase expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFLP3
FEATURES             Location/Qualifiers
     source          1..10719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      231..582
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             596..1429
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     primer_bind     1575..1597
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1589..1606
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1590..1606
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(2337..3671,3672..3758))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(3759..4204)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        join(4419..4424,4425..4441,4442..4447)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             4495..5685
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(4847..4866)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(6053..7324)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     RBS             7328..7336
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7425..8138
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     primer_bind     complement(8224..8240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8224..8240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8237..8259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8248..8264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8272..8278,8279..8296,8297..8302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8317..8338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8455..8472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            8722..8830
                     /note="incP origin of transfer"
     rep_origin      complement(8900..9488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8980..8999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9659..10450,10451..10519))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10282..10301
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10520..10624)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10692..10710
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatcctct caggcgccgc tggtgccgct ggttggacgc caagggtgaa tccgcctcga
      241 taccctgatt actcgcttcc tgcgccctct caggcggcga taggggactg gtaaaacggg
      301 gattgcccag acgcctcccc cgccccttca ggggcacaaa tgcggcccca acggggccac
      361 gtagtggtgc gttttttgcg tttccaccct tttcttcctt ttccctttta aaccttttag
      421 gacgtctaca ggccacgtaa tccgtggcct gtagagttta aaaagggacg gatttgttgc
      481 cattaaggga cggatttgtt gttaagaagg gacggatttg ttgttgtaaa gggacggatt
      541 tgttgtattg tgggacgcag atacagtgtc cccttataca caaggaatgt cgaacgtggc
      601 ctcaccccca atggtttaca aaagcaatgc cctggtcgag gccgcgtatc gcctcagtgt
      661 tcaggaacag cggatcgttc tggcctgtat tagccaggtg aagaggagcg agcctgtcac
      721 cgatgaagtg atgtattcag tgacggcgga ggacatagcg acgatggcgg gtgtccctat
      781 cgaatcttcc tacaaccagc tcaaagaagc ggccctgcgc ctgaaacggc gggaagtccg
      841 gttaacccaa gagcccaatg gcaaggggaa aagaccgagt gtgatgatta ccggctgggt
      901 gcaaacaatc atctaccggg agggtgaggg ccgtgtagaa ctcaggttca ccaaagacat
      961 gctgccgtac ctgacggaac tcaccaaaca gttcaccaaa tacgccttgg ctgacgtggc
     1021 caagatggac agcacccacg cgatcaggct ttacgagctg ctcatgcaat gggacagcat
     1081 cggccagcgc gaaatagaaa ttgaccagct gcgaaagtgg tttcaactgg aaggccggta
     1141 tccctcgatc aaggacttca agttgcgagt gcttgatcca gccgtgacgc agatcaacga
     1201 gcacagcccg ctacaggtgg agtgggcgca gcgaaagacc gggcgcaagg tcacacatct
     1261 gttgttcagt tttggaccga agaagcccgc caaggcggtg ggtaaggccc cagcgaagcg
     1321 caaggccggg aagatttcag atgctgagat cgcgaaacag gctcgccctg gtgagacatg
     1381 ggaagcggcc cgcgctcgac taacccagat gccgctggat ctggcctaga ggccgtggcc
     1441 accacggccc ggcctgcctt tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     1501 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     1561 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
     1621 cctgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc
     1681 catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat
     1741 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt
     1801 gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc
     1861 cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc
     1921 ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg
     1981 gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac
     2041 gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac
     2101 ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa
     2161 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccaaaagg
     2221 ttaggaatac ggttagccat ttgcctgctt ttatatagtt catatgggat tcacctttat
     2281 gttgataaga aataaaagaa aatgccaata ggatatcggc attttctttt gcgtttttat
     2341 ttgttaactg ttaattgtcc ttgttcaagg atgctgtctt tgacaacaga tgttttcttg
     2401 cctttgatgt tcagcaggaa gctaggcgca aacgttgatt gtttgtctgc gtagaatcct
     2461 ctgtttgtca tatagcttgt aatcacgaca ttgtttcctt tcgcttgagg tacagcgaag
     2521 tgtgagtaag taaaggttac atcgttagga tcaagatcca tttttaacac aaggccagtt
     2581 ttgttcagcg gcttgtatgg gccagttaaa gaattagaaa cataaccaag catgtaaata
     2641 tcgttagacg taatgccgtc aatcgtcatt tttgatccgc gggagtcagt gaacagatac
     2701 catttgccgt tcattttaaa gacgttcgcg cgttcaattt catctgttac tgtgttagat
     2761 gcaatcagcg gtttcatcac ttttttcagt gtgtaatcat cgtttagctc aatcataccg
     2821 agagcgccgt ttgctaactc agccgtgcgt tttttatcgc tttgcagaag tttttgactt
     2881 tcttgacgga agaatgatgt gcttttgcca tagtatgctt tgttaaataa agattcttcg
     2941 ccttggtagc catcttcagt tccagtgttt gcttcaaata ctaagtattt gtggccttta
     3001 tcttctacgt agtgaggatc tctcagcgta tggttgtcgc ctgagctgta gttgccttca
     3061 tcgatgaact gctgtacatt ttgatacgtt tttccgtcac cgtcaaagat tgatttataa
     3121 tcctctacac cgttgatgtt caaagagctg tctgatgctg atacgttaac ttgtgcagtt
     3181 gtcagtgttt gtttgccgta atgtttaccg gagaaatcag tgtagaataa acggattttt
     3241 ccgtcagatg taaatgtggc tgaacctgac cattcttgtg tttggtcttt taggatagaa
     3301 tcatttgcat cgaatttgtc gctgtcttta aagacgcggc cagcgttttt ccagctgtca
     3361 atagaagttt cgccgacttt ttgatagaac atgtaaatcg atgtgtcatc cgcattttta
     3421 ggatctccgg ctaatgcaaa gacgatgtgg tagccgtgat agtttgcgac agtgccgtca
     3481 gcgttttgta atggccagct gtcccaaacg tccaggcctt ttgcagaaga gatattttta
     3541 attgtggacg aatcgaactc aggaacttga tatttttcat ttttttgctg ttcagggatt
     3601 tgcagcatat catggcgtgt aatatgggaa atgccgtatg tttccttata tggcttttgg
     3661 ttcgtttctt tcgcaaacgc ttgagttgcg cctcctgcca gcagtgcggt agtaaaggtt
     3721 aatactgttg cttgttttgc aaactttttg atgttcatcg ttcatgtctc cttttttatg
     3781 tactgtgtta gcggtctgct tcttccagcc ctcctgtttg aagatggcaa gttagttacg
     3841 cacaataaaa aaagacctaa aatatgtaag gggtgacgcc aaagtataca ctttgccctt
     3901 tacacatttt aggtcttgcc tgctttatca gtaacaaacc cgcgcgattt acttttcgac
     3961 ctcattctat tagactctcg tttggattgc aactggtcta ttttcctctt ttgtttgata
     4021 gaaaatcata aaaggatttg cagactacgg gcctaaagaa ctaaaaaatc tatctgtttc
     4081 ttttcattct ctgtattttt tatagtttct gttgcatggg cataaagttg cctttttaat
     4141 cacaattcag aaaatatcat aatatctcat ttcactaaat aatagtgaac ggcaggtata
     4201 tgtgatgggt taaaaaggat cgatcctcta gagaatagga acttcggaat aggaacttca
     4261 agatcccctg attccctttg tcaacagcaa tggatactga ctcgctgcgc tcggtcgttc
     4321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4381 gggataacgc aggaaagaac atgaattaat tctcatgttt gacagcttat catcgattag
     4441 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gcgtatgaaa
     4501 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
     4561 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
     4621 agtcactatg gcgtgctgct ggcgctatat gcgttgatgc aatttctatg cgcacccgtt
     4681 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
     4741 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct ctacgccgga
     4801 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     4861 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     4921 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt acatgcacca
     4981 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     5041 gagtcgcata agggagagcg ccgacccatg cccttgagag ccttcaaccc agtcagctcc
     5101 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     5161 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     5221 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     5281 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     5341 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     5401 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     5461 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     5521 cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg
     5581 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     5641 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     5701 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     5761 tgaatgcgca aaccaaccct tggggctgca ggaattccat tgagtaagtt tttaagcaca
     5821 tcagcttcaa aagcgctctg aagttcctat actttctaga ggatccccct agagaatagg
     5881 aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc
     5941 gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata
     6001 tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc
     6061 gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg
     6121 cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct
     6181 caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat
     6241 agtaccgaga aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg
     6301 ttgtcctggc cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact
     6361 ccgttaggcc cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg
     6421 ggccattttt tatagcaaag attgaataag gcgcattttt cttcaaagct ttattgtacg
     6481 atctgactaa gttatctttt aataattggt attcctgttt attgcttgaa gaattgccgg
     6541 tcctatttac tcgttttagg actggttcag aattcctcaa aaattcatcc aaatatacaa
     6601 gtggatcgat cctacccctt gcgctaaaga agtatatgtg cctactaacg cttgtctttg
     6661 tctctgtcac taaacactgg attattactc ccagatactt attttggact aatttaaatg
     6721 atttcggatc aacgttctta atatcgctga atcttccaca attgatgaaa gtagctagga
     6781 agaggaattg gtataaagtt tttgtttttg taaatctcga agtatactca aacgaattta
     6841 gtattttctc agtgatctcc cagatgcttt caccctcact tagaagtgct ttaagcattt
     6901 ttttactgtg gctatttccc ttatctgctt cttccgatga ttcgaactgt aattgcaaac
     6961 tacttacaat atcagtgata tcagattgat gtttttgtcc atagtaagga ataattgtaa
     7021 attcccaagc aggaatcaat ttctttaatg aggcttccag aattgttgct ttttgcgtct
     7081 tgtatttaaa ctggagtgat ttattgacaa tatcgaaact cagcgaattg cttatgatag
     7141 tattatagct catgaatgtg gctctcttga ttgctgttcc gttatgtgta atcatccaac
     7201 ataaataggt tagttcagca gcacataatg ctattttctc acctgaaggt ctttcaaacc
     7261 tttccacaaa ctgacgaaca agcaccttag gtggtgtttt acataatata tcaaattgtg
     7321 gcatacaacc tccttagtac atgcaaccat tatcaccgcc agaggtaaaa tagtcaacac
     7381 gcacggtgtt agatatttat cccttgcggt gatagattta acgtatgagc acaaaaaaga
     7441 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa
     7501 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt
     7561 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc
     7621 ttacaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct
     7681 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg
     7741 ttttttctca tgttcaggca gggatgttct cacctaagct tagaaccttt accaaaggtg
     7801 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg
     7861 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt
     7921 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg
     7981 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac
     8041 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag
     8101 ttatcgctag tcagtggcct gaagagacgt ttggctgatc ggcaaggtgt tctggtcggc
     8161 gcatagctga taacaattga gcaagaatct tcatcggggt accgagctcg aattcgtaat
     8221 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8281 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8341 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8401 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgg ggattcctta
     8581 aggtatactt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata
     8641 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
     8701 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
     8761 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
     8821 aggctggccg ataagctagc ttgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8881 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8941 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9001 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9061 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9121 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9181 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9241 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9301 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9361 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9421 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9481 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9541 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9601 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9661 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9721 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9781 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9841 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9901 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9961 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10021 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10081 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10141 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10201 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10261 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10321 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10381 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10441 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10501 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10561 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10621 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    10681 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
