LOCUS       sequence-35096-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Ra HJ, Harju-Baker S, Zhang F, Linhardt RJ, Wilson CL, Parks WC
  TITLE     Control of promatrilysin (MMP7) activation and substrate-specific 
            activity by sulfated glycosaminoglycans.
  JOURNAL   J Biol Chem. 2009 Oct 9. 284(41):27924-32.
  PUBMED    19654318
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Oct 9. 284(41):27924-32."
COMMENT     Sequence Label: pET23b-proMMP-7-E215Q
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             812..829
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgctgccg ctgcctcagg aggcgggagg
       61 catgagtgag ctacagtggg aacaggctca ggactatctc aagagatttt atctctatga
      121 ctcagaaaca aaaaatgcca acagtttaga agccaaactc aaggagatgc aaaaattctt
      181 tggcctacct ataactggaa tgttaaactc ccgcgtcata gaaataatgc agaagcccag
      241 atgtggagtg ccagatgttg cagaatactc actatttcca aatagcccaa aatggacttc
      301 caaagtggtc acctacagga tcgtatcata tactcgagac ttaccgcata ttacagtgga
      361 tcgattagtg tcaaaggctt taaacatgtg gggcaaagag atccccctgc atttcaggaa
      421 agttgtatgg ggaactgctg acatcatgat tggctttgcg cgaggagctc atggggactc
      481 ctacccattt gatgggccag gaaacacgct ggctcatgcc tttgcgcctg ggacaggtct
      541 cggaggagat gctcacttcg atgaggatga acgctggacg gatggtagca gtctagggat
      601 taacttcctg tatgctgcaa ctcatcaact tggccattct ttgggtatgg gacattcctc
      661 tgatcctaat gcagtgatgt atccaaccta tggaaatgga gatccccaaa attttaaact
      721 ttcccaggat gatattaaag gcattcagaa actatatgga aagagaagta attcaagaaa
      781 gaaatagtga aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      841 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctga
//
