LOCUS       sequence-351002-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-CHMP4C(121-233, S191C)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-Delta-N-CHMP4C(S191C)
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             175..192
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             193..204
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             262..282
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggatccatgc agtataaact ggcgctgaac ggcaaaaccc tgaaaggcga aaccaccacc
       61 gaagcggtgg atgcggcgac cgcggaaaaa gtgtttaaac agtatgcgaa cgataacggc
      121 gtggatggcg aatggaccta tgatgatgcg accaaaacct ttaccgtgac cgaactggtg
      181 ccgcgcggca gcattgaagg ccgcattcgc gaaaacctga aagatagcgg cctgtttctg
      241 gaacatcatc atcatcatca tgaaaacctg tattttcaag gcagcgaaaa catggatctg
      301 aacaaaattg atgatctgat gcaagaaatt accgaacagc aagatattgc gcaagaaatt
      361 agcgaagcgt ttagtcagcg cgtgggcttt ggcgatgatt ttgatgaaga tgaactgatg
      421 gcggaactgg aagaactgga acaagaagaa ctgaacaaaa aaatgaccaa tattcgcctg
      481 ccgaacgttc cgagctgcag cctgccggct cagccgaacc gcaaaccggg catgagcagc
      541 actgcgcgtc gctcgcgggc ggcgagcagt cagcgcgcgg aagaagaaga tgatgatatt
      601 aaacagctgg cggcgtgggc gacctaaaag ctt
//
