LOCUS       sequence-351004-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-CHMP4B(121-224, S184C)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-Delta-N-CHMP4B(S184C)
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             175..192
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             193..204
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             262..282
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggatccatgc agtataaact ggcgctgaac ggcaaaaccc tgaaaggcga aaccaccacc
       61 gaagcggtgg atgcggcgac cgcggaaaaa gtgtttaaac agtatgcgaa cgataacggc
      121 gtggatggcg aatggaccta tgatgatgcg accaaaacct ttaccgtgac cgaactggtg
      181 ccgcgcggca gcattgaagg ccgcattcgc gaaaacctga aagatagcgg cctgtttctg
      241 gaacatcatc atcatcatca tgaaaacctg tattttcaag gcagcgataa catggatatt
      301 gataaagtgg acgaattaat gcaagatatt gcggatcagc aagaactggc ggaagaaatt
      361 agcaccgcga ttagcaaacc ggtgggcttt ggcgaagaat ttgatgaaga tgagctgatg
      421 gcggaactgg aagagctgga acaagaagaa ctggataaaa acctgctgga aatttgcggc
      481 ccggaaaccg tgccgctgcc gaacgtgccg agcattgcgc tgccgagcaa accggcgaaa
      541 aaaaaagaag aagaagatga tgatatgaaa gaactggaaa actgggcggg cagcatgtaa
      601 aagctt
//
