LOCUS       sequence-351016-        2889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates 
            expression in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-ALIX(P801G)
FEATURES             Location/Qualifiers
     source          1..2889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..183
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             184..195
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             235..252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..273
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cagtataaat tagcactgaa cggcaaaacc ctgaaaggcg aaaccaccac cgaagcggtg
       61 gatgcggcga ccgcggaaaa agtgtttaaa cagtatgcga acgataacgg cgtggatggc
      121 gaatggacct atgatgatgc gaccaaaacc tttaccgtaa ccgaactggt gccccgcggc
      181 agcatagaag gccgcattcg cgaaaacctg aaagatagcg gcctgtttct ggaacatcat
      241 catcatcatc atgaaaacct gtattttcaa ggcagcggca gcatggcgac ctttattagc
      301 gtgcagctga aaaaaacgag cgaagtggat ctggcgaaac cgctggtgaa atttattcag
      361 cagacctatc cgagcggcgg cgaagaacaa gcgcagtatt gtcgcgcagc tgaagagctg
      421 agcaaattgc gccgcgccgc ggttggtcgc ccgctggata aacatgaagg cgcgctggaa
      481 accctgctgc gctattatga tcagatttgc agcattgaac cgaaatttcc gtttagcgaa
      541 aatcagattt gcctgacctt tacctggaaa gatgcgtttg ataaaggcag cctgtttggc
      601 ggcagcgtga aactggctct ggctagcctg ggctatgaaa aaagctgcgt gctgtttaac
      661 tgcgcggccc tggcgagtca gattgcggcg gaacagaacc tggataacga tgaaggcctg
      721 aaaattgcag cgaagcatta ccaatttgcg agcggcgcgt ttctgcatat taaagaaacc
      781 gtgctgagcg cgctgagccg cgaaccgacc gtggatatta gcccggatac cgtgggcacc
      841 ctgagcctga ttatgctggc gcaagcacaa gaagtgtttt ttctgaaagc gacccgcgat
      901 aaaatgaaag atgcgattat tgcgaaactg gcgaaccaag cggcggatta ttttggcgat
      961 gcgtttaaac agtgtcagta taaagatacc ctgccgaaag aagtgtttcc ggtgctggcg
     1021 gcgaaacatt gtataatgca agcgaacgcg gaatatcacc aaagtatctt ggcgaagcag
     1081 cagaaaaaat ttggcgaaga aattgcgcgc ctgcagcatg cggcggaact gattaaaacc
     1141 gtggcgagcc gctatgatga atatgtgaac gtgaaagatt ttagcgataa aattaaccgc
     1201 gcgctggcgg cggcgaaaaa agataacgat tttatttatc atgatcgcgt gccggatctg
     1261 aaagacctgg acccgattgg caaagcgacc ctggtgaaaa gcaccccggt gaacgtgccg
     1321 attagtcaga aatttaccga tctgtttgaa aaaatggtgc cggtgagcgt gcagcagagc
     1381 ctggcggcgt ataatcagcg caaagcggat ctggtgaacc gcagcattgc gcagatgcgc
     1441 gaagcgacca ccctggcgaa cggcgtgctg gcgagcctga acctgccggc ggcgattgaa
     1501 gatgtgagcg gcgataccgt gccgcaatct atcctgacca aaagccgcag cgtgattgaa
     1561 caaggcggca ttcagaccgt ggatcagctg attaaagaac tgccggaact gctgcaacgc
     1621 aatcgcgaaa ttctggatga aagcctgcgc ctgctggatg aagaagaagc gaccgataac
     1681 gatctgcgcg cgaaatttaa agaacgctgg cagcgcaccc cgagcaacga actgtataaa
     1741 ccgctgcgcg cggaaggcac caactttcgc accgtgctgg ataaagcggt gcaagcggat
     1801 ggccaagtga aagaatgcta tcagagccat cgcgatacca ttgtgctgct gtgcaaaccg
     1861 gaaccggaac tgaacgcggc gattccgagc gcgaacccgg cgaaaaccat gcaaggcagc
     1921 gaagtggtga acgtgctgaa aagcctgctg agcaacctgg atgaagtgaa aaaagaacgc
     1981 gaaggcctgg aaaacgatct gaaaagcgtg aactttgata tgacgagcaa atttctgacc
     2041 gcgctggcgc aagatggcgt gattaacgaa gaagcgctga gcgtgaccga actggatcgc
     2101 gtgtatggcg gcctgaccac caaagtgcaa gaaagcctga aaaaacaaga aggcctgctg
     2161 aaaaacattc aagtgagcca tcaagaattt agcaaaatga aacagagcaa caacgaagcg
     2221 aacctgcgcg aagaagtgct gaaaaacctg gcgaccgcgt atgataactt tgtggaactg
     2281 gtggcgaacc tgaaagaagg caccaaattt tataacgaac tgaccgaaat tctggtgcgc
     2341 tttcagaaca aatgcagcga tattgtgttt gcgcgcaaaa ccgaacgcga cgaactgctg
     2401 aaagatctgc agcagagcat tgcccgcgaa ccaagcgccc caagcatccc gacgccagcc
     2461 tatcagagta gtccggcggg tggtcatgcg ccgaccccac caacgccggc cccacgtacg
     2521 atgccgccaa ccaaaccgca accaccagcg cgtccgccac caccagttct cccagccaat
     2581 cgcgccccaa gtgcgaccgc gccaagccca gttggtgcgg gtaccgccgc gccagcgcca
     2641 agtcaaacgc cgggtagtgc cccaccacca caagcgcaag gcggcccata tccgacgtac
     2701 ccgggctatc cgggctactg ccagatgcca atgccgatgg gttacaaccc atacgcgtac
     2761 ggccagtata acatgccgta cccaccggtg taccaccaga gtccgggcca agccccatac
     2821 ccgggtccac agcagccaag ctacccgttc ccgcagccac cacagcaaag ttactacccg
     2881 cagcaataa
//
