LOCUS       sequence-351314-        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  the plasmid promotes the constitutive expression of CreERT2 to 
            enable intracellular recombination, Gaussia Luc can be used for in 
            vivo imaging and mCherry is used as a marker for ex vivo analyses..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Carlet M, Volse K, Vergalli J, Becker M, Herold T, Arner A, Senft D,
            Jurinovic V, Liu WH, Gao Y, Dill V, Fehse B, Baldus CD, Bastian L, 
            Lenk L, Schewe DM, Bagnoli JW, Vick B, Schmid JP, Wilhelm A, 
            Marschalek R, Jost PJ, Miething C, Riecken K, Schmidt-Supprian M, 
            Binder V, Jeremias I
  TITLE     In vivo inducible reverse genetics in patients' tumors to identify 
            individual therapeutic targets.
  JOURNAL   Nat Commun. 2021 Sep 27;12(1):5655. doi: 10.1038/s41467-021-25963-z.
  PUBMED    34580292
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-27"; volume: "12(1):5655. doi"; pages: " 
            10.1038/s41467-021-25963-z"
COMMENT     Sequence Label: pCDH-SFFV-GLuc-CreERT2-mCherry
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2259..2268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3078..3131
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3138..4166
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4170..5105
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4218..5108
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             5115..5171
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5178..5888
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5323..5341)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5591..5610
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5785..5808
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5895..6483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5948..5968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6366..6377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6557..6790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6862..6983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6899..6918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6953..6972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7002..7137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7170..7186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7170..7186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7183..7205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7194..7210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7218..7224,7225..7242,7243..7248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7263..7284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7401..7418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7572..8160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8331..9122,9123..9191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8954..8973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9192..9296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9364..9382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9420..9442)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9542..9561
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9755..9777
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9769..9786
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9770..9786
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtgatca agggcgggta catgaaaata gctaacgttg ggccaaacag
     1981 gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag
     2041 tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca
     2101 gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc
     2161 cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg
     2221 agctctataa aagagctcac aacccctcac tcgaattcgc caccatggct ctcccagtga
     2281 ctgccctact gcttccccta gcgcttctcc tgcatgccaa gcccaccgag aacaacgaag
     2341 acttcaacat cgtggccgtg gccagcaact tcgcgaccac ggatctcgat gctgaccgcg
     2401 ggaagttgcc cggcaagaag ctgccgctgg aggtgctcaa agagatggaa gccaatgccc
     2461 ggaaagctgg ctgcaccagg ggctgtctga tctgcctgtc ccacatcaag tgcacgccca
     2521 agatgaagaa gttcatccca ggacgctgcc acacctacga aggcgacaaa gagtccgcac
     2581 agggcggcat aggcgaggcg atcgtggaca ttcctgagat tcctgggttc aaggacttgg
     2641 agccaatgga gcagttcatc gcacaggtcg atctgtgtgt ggactgcaca actggctgcc
     2701 tcaaagggct tgccaacgtg cagtgttctg acctgctcaa gaagtggctg ccgcaacgct
     2761 gtgcgacctt tgccagcaag atccagggcc aggtggacaa gatcaagggg gccggtggtg
     2821 acttagcggc cgcacccacc acgacgccag cgccgcgacc accaaccccg gcgcccacga
     2881 tcgcgtcgca gcccctatcc ctgcgcccag aggcgtgccg gccagcggcg gggggcgcag
     2941 tgcacacgag ggggctggac ttcgcctgtg atatctacat ctgggcgccc ctggccggga
     3001 cttgtggggt ccttctcctg tcactggtta tcacccttta ctgcaaccac aggcggattc
     3061 aaggatccgc ggccgctgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     3121 atcccggccc tatgcatatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac
     3181 cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc
     3241 aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat
     3301 ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc
     3361 ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc
     3421 taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac
     3481 tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc
     3541 tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct
     3601 gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag
     3661 ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa
     3721 tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc
     3781 tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga
     3841 ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc
     3901 agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg
     3961 ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag
     4021 ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct
     4081 ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa
     4141 tggtgcgcct gctggaagat ggcgattctg ctggagacat gagagctgcc aacctttggc
     4201 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
     4261 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
     4321 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
     4381 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
     4441 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
     4501 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
     4561 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     4621 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     4681 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     4741 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     4801 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     4861 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     4921 tggtgcccct ctatgacctg ctgctggagg cggccgacgc ccaccgccta catgcgccca
     4981 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     5041 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     5101 ccacagtccc cggggccacg aacttctctc tgttaaagca agcaggagat gttgaagaaa
     5161 acccagggcc tatgcacatg gtgagcaagg gcgaggagga caacatggcc atcatcaagg
     5221 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     5281 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5341 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     5401 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5461 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5521 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     5581 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     5641 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     5701 gcggccacta cgacgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     5761 ccggcgccta caacgtcaac atcaagctgg acatcacctc ccacaacgag gactacacca
     5821 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     5881 acaagtaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5941 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6001 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6061 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6121 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6181 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6241 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6301 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6361 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6421 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6481 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6541 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt
     6601 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6781 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6841 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6961 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7141 acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     7201 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7261 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7381 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7441 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7501 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8041 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8221 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8281 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9361 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     9421 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9481 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     9541 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     9601 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     9661 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9721 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9781 gccagtgcca agctg
//
