LOCUS       sequence-351316-        9335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KSHV ORF45 tagged, double stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0016L
FEATURES             Location/Qualifiers
     source          1..9335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2625..2634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3852..3917
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3953..3977)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4030..4071
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4095..4683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4148..4168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4566..4577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4698..5014
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4865..5000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5065..5067,5068..5070,5071..5784)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5110..5131)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5371..5390)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5718..5739
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5856..6089
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6161..6282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6198..6217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6252..6271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6322..6457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6477..6496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6478..6496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6506..6523)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6506..6522)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6515..6537)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6664..7119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6751..6770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6961..6982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7145..7249
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7250..7318,7319..8110)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7468..7487)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8281..8869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8770..8789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9023..9040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9157..9178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9193..9198,9199..9216,9217..9223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9231..9247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9236..9258
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9255..9271
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9255..9271
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9290..9310
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9292..9310
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg ctctttaaag gaaccaattc agtcgactgg atccgccacc atggcgatgt
     2641 ttgtgaggac ctcgtctagc acacacgatg aagagagaat gcttccaatt gaaggagcgc
     2701 ctcgcagacg accccccgtg aagttcatat tcccacctcc acctctttca tcacttccag
     2761 gatttggcag gccgcgcggc tatgctggac ccacggtgat agatatgtct gccccagacg
     2821 acgtcttcgc cgaggacacg ccatcgccgc cagcaacccc tctggatcta cagatatccc
     2881 cggatcagtc gagcggcgaa tctgaatatg acgaggatga ggaagatgaa gatgaagaag
     2941 aaaatgacga tgttcaggag gaagacgagc cagaggggta ccctgcagac ttttttcaac
     3001 ctttatctca cttgcgcccg aggcctctgg ccagacgggc ccatacgccc aaaccggtag
     3061 cagtggtagc gggccgcgtg cgcagttcaa cggacacggc ggagtccgag gcgtccatgg
     3121 gatgggttag tcaggatgac ggattttccc ctgctgggct ctcaccttca gacgacgagg
     3181 gggttgctat cctggaaccg atggcggcat acactgggac cggggcatac ggactttcac
     3241 ctgcttccag aaatagtgta cctggaacac aaagttcacc atacagcgac cctgatgaag
     3301 ggccctcgtg gcgccccctg cgcgccgcac ccaccgcgat cgtcgacctg acatcggact
     3361 ctgatagcga tgacagttcc aactctccgg acgtgaacaa tgaggccgcg tttaccgacg
     3421 cgcgccattt ttcccaccag ccaccctcgt ccgaggagga cggagaagac caaggggaag
     3481 tattgagtca gagaatcggg ctcatggacg tgggccagaa gcgcaaaagg cagtctaccg
     3541 cctcctctgg tagcgaggat gtggtgcgct gccagagaca accaaactta agccgcaaag
     3601 cagtggcgtc cgtgataatt atatcctcgg ggagtgacac agacgaggag ccctcgtccg
     3661 ccgtgagcgt gatcgtgtct ccgtcgagca caaagggtca cctcccaacc caatctccca
     3721 gtacttccgc ccactcgatt tcatcaggaa gcacaactac cgcggggtcc aggtgcagcg
     3781 acccaacccg catcctggcc tccacgccac ccctgtgtgg aaacggtgca tataactggc
     3841 cgtggctgga cgactacaaa gaccatgacg gtgattataa agatcatgat atcgattaca
     3901 aggatgacga tgacaagtag taactcgagg cggccgcact cgagatatct agacccagct
     3961 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     4021 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     4081 tagtaatgat cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4141 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4201 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4261 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4321 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4381 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4441 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4501 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4561 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4621 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4681 cgcctggcga tggtaccggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4741 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4801 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4921 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4981 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5041 tttcggatct gatcagcacc tagcatggtg agcaagggcg aggagctgtt caccggggtg
     5101 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5161 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     5221 aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc
     5281 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5341 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5401 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5461 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
     5521 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
     5581 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5641 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5701 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5761 ggcatggacg agctgtacaa gtaaggtacc tttaagacca atgacttaca aggcagctgt
     5821 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5881 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5941 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6001 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6061 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6121 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6241 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6301 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6421 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6481 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6541 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6601 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6661 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6961 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7021 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7081 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7141 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7201 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7261 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7321 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7381 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7441 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7501 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7561 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7621 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7681 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7741 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7801 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7861 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7921 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7981 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8041 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8101 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8161 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8221 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8701 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8881 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8941 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9001 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9061 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9121 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9181 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9241 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     9301 tcactaaagg gaacaaaagc tggagctgca agctt
//
