LOCUS       sequence-351442-        8331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KSHV ORF50 hyp tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0180
FEATURES             Location/Qualifiers
     source          1..8331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    914..938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3043..3108
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3144..3168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3179..3181,3182..3184,3185..3898)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3212..3240)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3835..3855
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3920..3937)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3926..4150
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4196..4624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4283..4302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4493..4514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4633..4653)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4638..4967
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4818..4953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4880..4899
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5034..5828
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5088..5107)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5698..5717
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6004..6125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6041..6060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6095..6114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6174..6190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6174..6190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6187..6209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6198..6214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6222..6228,6229..6246,6247..6252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6267..6288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6405..6422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6576..7164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6656..6675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7335..8126,8127..8195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7958..7977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8196..8300)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttgat caaacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc
      961 gacgccacca tggcgcaaga tgacaagggt aagaagcttc ggcggtcctg tgtggaaagc
     1021 ttcgtcggcc tctcggacga actgaaggcc caactctacc agtgtgtgct ccttataaat
     1081 gacgcatacg aaacaatcta cgatcccagt gacctaaata gagtggtgga agatgtgtgc
     1141 attcggatta tgaaagaatg ttccaagctt ggtgcgctat gtggtctgtt tacagacatt
     1201 aacatgttta accttttctg cttttttcgt gcctctcgaa tgaggaccaa aggcgcggcc
     1261 gggtacaacg tgccatgcgc agaggcatcc caaggcatta ttcggatcct cacggagagg
     1321 atcttattct gcacagaaaa ggcatttctg acagccgcat gcagcggggt gagcctgcct
     1381 ccagccatat gtaagctact acacgaaata tacactgaaa tgaaggccaa atgcctgggg
     1441 gcctggaggc gactcgtctg caatcggagg cccattatga tattaacctc ttccctactg
     1501 aagctctaca acacgtacga taccgccggg ctgctctctg agcagtccag ggccctctgc
     1561 cttttggttt tccaaccggt ctaccttccg aggattatgg cgccgctgga gatcatgacc
     1621 aagggtcagc tcgcccctga aaacttttac agcatcaccg gttctgctga gaaacgccgg
     1681 ccaattacca ccggcaaggt cactggactg tcctatccag gaagcggtct catgccagaa
     1741 tctttaattt tgccaatcct ggagccagga ctgttgccgg cttccatggt agacctcagc
     1801 gatgtgctgg caaaacccgc cgttattctg agcgcccctg ccctgagcca gtttgtcatt
     1861 agcaaacccc atcccaacat gccgcacacc gtcagcatca tcccctttaa cccatcgggt
     1921 acagacccgg cgtttattag tacgtggcag gccgcgtcac agaatatggt gtacaacaca
     1981 tccaccgcgc ccttaaaacc ggccaccggt agttcacaga cggtgtcagt caaggcggtt
     2041 gctcaagggg ccgtgattac tgcgacaacg gtgccgcagg caatgccagc gcggggtacc
     2101 ggaggggagt tgcctgtaat gtcagcgtcc actcctgcaa gagatcaggt cgctgcatgt
     2161 tttgtcgcag agaacaccgg agattctccc gacaacccga gctctttcct gacgtcatgt
     2221 cacccttgcg atccgaacac ggttatagtg gcccagcaat ttcaaccacc gcaatgcgtt
     2281 acgttgttgc aggttacctg tgccccctct tcgacaccac cccccgattc aacagtccgg
     2341 gccccggtgg tgcagttgcc aacagtagtc cctctgccgg ccagcgcgtt cctcccggcg
     2401 ctcgcccaac cagaagcctc gggcgaagag cttccgggcg gtcatgacgg agaccaaggt
     2461 gtgccgtgta gagattcaac ggcggcggct acggcggcag aggcgacaac acccaaacga
     2521 aagcagagaa gcaaagagag gagctcaaag aagcgtaagg ctttgaccgt gccagaagcc
     2581 gacaccacgc catcgaccac gacacctggt acctctttgg gatcaattac caccccccag
     2641 gatgtgcacg ccacggatgt cgccacgtct gagggaccat cggaggcaca acccccgcta
     2701 ctgtcgttac ccccgccact ggacgtagat cagagtctat tcgccctgtt agacgaagcg
     2761 ggccctgaaa catgggatgt cgggtcgcct ctctccccca ctgacgacgc gctgttgtcc
     2821 agtattctgc aaggactgta ccagctggac acgccaccgc ctctgcggtc accctccccc
     2881 gcttccttcg gcccggagtc tccggcggat ataccgtcac cttctggtgg agagtatacg
     2941 caactgcaac cggtcagggc gacctcggcg acgcccgcta acgaggtaca ggagtccggc
     3001 acactgtacc agctgcacca atggcgtaat tacttccgag acgactacaa agaccatgac
     3061 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagta gtaactcgag
     3121 gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttc gatctagaat
     3181 ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     3241 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg ctacatacgg
     3301 aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     3361 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
     3421 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
     3481 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
     3541 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
     3601 actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
     3661 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc aactagcaga
     3721 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     3781 cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct
     3841 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaataatg
     3901 aattaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3961 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4021 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4081 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4141 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4261 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4381 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4501 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4561 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4621 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4741 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4981 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     5041 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5101 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5161 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5221 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5281 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5341 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5401 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5461 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5521 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5581 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5641 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5701 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5761 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5821 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca
     5881 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5941 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6001 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6121 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
