LOCUS       sequence-351789-        4872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal 
            Modular Cloning ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK088
FEATURES             Location/Qualifiers
     source          1..4872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagcag tatgcttgat gttatccaaa gtgtgagaga ggaaggtcca
     2281 aacatacacg attgggagag ggcctaggta taagagtttt tgagtagaac gcatgtgagc
     2341 ccagccatct cgaggagatt aaacacgggc cggcatttga tggctatgtt agtaccccaa
     2401 tggaaagcct gagagtccag tggtcgcaga taactcccta aattccctga gctaactcta
     2461 agtcgaccat gccgtttatg gttagcgcct cccaaaaagg aatggccgac ttagagttac
     2521 ctcttgaccg actttttctt tcctccccct tacatttcgt taccacaaca cattcctata
     2581 tccaaactcc aggtacataa ctagtcgaaa tctcttttaa atctagtcaa gaactagata
     2641 cttaaccttc atactgctta gagatagcct ttgaagctct tatttcgctc tttgtctcac
     2701 tctcaccttc ctcctcctcc tccaatcctt tttgctcggg actagtccaa gactagtcaa
     2761 cgattagtat gccgagcatt cgcgataacg atctccgaaa gtccccagag tactgtcact
     2821 atctcgaggc agttaaggac ggggagctta cgctgccgga tttcaagata gtaagccgac
     2881 ccgactagct ttaagcctag ttactgacta gtccacagga cgacaatggc gtgcctgata
     2941 tccatccata tgaagtctac tgccgagtga agggatgcct caagcgtaca gtgagtctac
     3001 tgctatacta gtttctgact agttattaac tagttgacag gttccctctg ccaacagaaa
     3061 catattggtc aagcacttga aggacaagaa ctcccacggc atggagttta cattgcacaa
     3121 tggacctccc actatgaagg aactgatgga ggccaaaggc aagtcctatc tagattactt
     3181 ggtgactagt ccctgactag tcaagtagca tggtatgaag gcttgtttga aggcactgtt
     3241 ctcccaaccc cgactcctac caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta
     3301 actagtgtat tagctaatat atagcagcac caagtccaag gaccacaata ctaagggtgt
     3361 cgagaattcg tgagtttctt ctcccatttc aactagtcct tgactagtca ctaactactt
     3421 tgcagcaacg agggtgaagc tggaaatgat caggacaatg gcgagggccc gtaagtacag
     3481 ccattcaatg cagactagtt gctaactaat ctgtgactag ttcaagtggt ccgtacgccg
     3541 tgcatacccc tgtgactggt aggaatttga gcaagcctgt cttgccgcgc gatgagaaag
     3601 gaaaggcaag ttacattcag ccccgtacct aggatcagtg ctaatttata acctctagcc
     3661 actctttatg cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg
     3721 aaccataccc tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt
     3781 ttgggactag attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt
     3841 ggcgcttttt ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag
     3901 actcagacaa agaccaacta gttattgacc agtcatctag aaggctctgt tgtggatctt
     3961 gaggccctgg agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca
     4021 ctaactagac tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa
     4081 agtggtaaga catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa
     4141 acaaggaaaa tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg
     4201 gtgatggcag acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa
     4261 taactagtct ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat
     4321 aatagtagta agttattcta gcttcagagt tataggagac tagatactaa ctagtattag
     4381 ttgcaactaa cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa
     4441 ctagactgtg gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc
     4501 tccctaatat gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc
     4561 cttctaggtt ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt
     4621 ttctaactag tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg
     4681 tttatacgtc aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt
     4741 ttataattag tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa
     4801 caagataccg tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctcgct
     4861 tgagaccacg aa
//
