LOCUS       sequence-351795-       10587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Lmnb1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10587)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV V5-lamin B1
FEATURES             Location/Qualifiers
     source          1..10587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5381..5930
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5525..5542)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5752..5771
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5933..6532
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5933..5952)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6429..6449
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6556..7144
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6609..6629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7027..7038)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7351..7984
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8112..8128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8112..8128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8125..8147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8136..8152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8160..8166,8167..8184,8185..8190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8205..8226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8343..8360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8514..9102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8594..8613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9273..10064,10065..10133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9896..9915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10134..10238)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10286..10420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10323..10342)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10377..10396
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggcgaccgcg acccccgtgc
     3481 agcagcagcg ggcgggcagc cgcgccagcg cccccgccac gccgctcagc cccacgcgcc
     3541 tgtcgcgcct gcaggagaaa gaggagctgc gggagctcaa cgaccgcctg gctgtgtaca
     3601 tcgataaggt gcgcagcctg gagacggaga acagcgcgct gcagctgcag gtgaccgagc
     3661 gcgaggaggt gcgcggccgc gagctcaccg gcctcaaggc tctctacgag accgagctgg
     3721 ccgacgcacg ccgcgctctg gacgacacgg cccgcgagcg cgccaagctc cagatcgagc
     3781 tgggcaagtt caaggccgag cacgaccagc tgctgctcaa ttatgccaag aaggaatctg
     3841 atctcagtgg agcccagatc aagcttcgag agtatgaggc ggcactaaac tctaaggatg
     3901 cggcgctggc tactgcccta ggggacaaaa agagtttaga gggagacttg gaggatctga
     3961 aagatcagat tgcccagcta gaagcatcct tatctgccgc caaaaagcag ttagcagatg
     4021 aaactttact taaagtggat ttggagaatc gctgtcagag ccttactgag gacttggagt
     4081 ttcgtaaaaa tatgtatgaa gaggagatca atgagacaag gaggaagcat gagacccgct
     4141 tggtggaagt ggactctggg cgtcagattg agtatgagta caagctggct caagccctgc
     4201 atgagatgcg ggagcagcac gacgcgcagg tgaggctgta caaggaagag ctggagcaga
     4261 cctaccacgc caagcttgag aatgccagac tctcctcaga gatgaacact tccactgtca
     4321 acagtgcccg ggaagagctg atggagagcc ggatgaggat cgagagcctc tcctcacagc
     4381 tctctaacct gcagaaagag tctagagcgt gtttggaaag gatccaggaa ttggaggaca
     4441 tgcttgctaa ggagagagac aactcgcgcc gcatgctgtc tgacagagag agagagatgg
     4501 cggagatcag ggaccagatg cagcagcagc tgagtgatta tgagcagctg ctggacgtga
     4561 agctggccct ggacatggag atcagcgcct acaggaagct cctggaaggc gaagaagagc
     4621 ggttaaagct ctctccaagc ccttcttccc gggtgaccgt gtccagagcg tcctccagtc
     4681 gcagtgtgcg caccaccaga ggaaagcgga agagagttga tgtggaggag tcggaggcga
     4741 gcagcagtgt tagcatttcc cactctgcct cagccacggg gaacgtgtgc attgaagaga
     4801 tagatgttga tgggaagttt attcgcttga agaacacttc tgagcaggat caaccaatgg
     4861 gaggctggga gatgatcaga aaaattggag acacatcagt cagttacaaa tatacctcaa
     4921 gatatgtgct gaaggctggc cagactgtca cagtgtgggc tgcaaatgct ggcgtcacag
     4981 ccagccctcc aactgacctc atctggaaga accagaactc ttggggtact ggtgaagatg
     5041 tgaaggttat attgaagaat tctcagggag aggaggttgc tcagagaagc acagtcttca
     5101 agaccaccat acccgaggag gaggaggagg aggaggagga gcccatcgga gtggctgtgg
     5161 aggaggagcg tttccaccag cagggagccc caagagcatc caataaaagc tgtgccatta
     5221 tgtgaacttg taatgggccg cgacgatgtt taaacttcga aacgcgtgaa ttcctcgagg
     5281 gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac
     5341 ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc
     5401 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     5461 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     5521 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     5581 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     5641 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     5701 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     5761 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     5821 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     5881 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgaccga
     5941 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6001 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6061 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6121 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6181 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6241 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6301 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6361 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6421 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6481 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacccgggcg
     6541 gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6601 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6661 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6721 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6781 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6841 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6901 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     6961 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7021 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7081 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7141 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     7201 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7261 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7321 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     7381 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7441 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7501 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7561 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7621 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7681 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7741 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     7801 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7861 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7921 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7981 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8041 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     8101 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8161 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8221 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8281 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8941 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10261 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10321 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10381 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10441 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10501 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10561 ttaaatatgt actacaaact tagtagt
//
