LOCUS       sequence-35199-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     Ramakrishnan Lab Plasmids
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pR2HYG
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     195..211
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     196..212
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(777..793)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(777..793)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc cctcgatctc
       61 gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga actcggtgat
      121 gacgttctcg gaggaggcca tatgtatatc tccttcttaa atctagattt aaagatctgg
      181 taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
      241 cgggggatca tctgatttag gcccctttcg tcactccatg ctaacacgcc cccctaacga
      301 ctgcggtcca acggtgcagg tcaggggcat cgcggctcaa cattgggggt tgacaggagg
      361 tgcaagacgt gcatcgtcca gccctgttcg cggccgcttc gacgaccccc gacacgccca
      421 cagccagcga acatgtgacc gcccacccaa acccgacaac cgggaagaca tcccgtctca
      481 ctacggctgg accaagccga ggctcaccac cccggcggcc ctggtcggga cgccgatcag
      541 gcgggtctgg cgccaaccat cgcgacgcca cgacggcgcc gaggccgcct gcgctaccgt
      601 ccgcggcggc tacctactac tgtgcatagt cgaatcggtg tcagcacgcc gtcatgtgat
      661 cgacgatgct ccgaacccag cgggaggctt cgagtgaacc tgttcaaaat ccgcctcgta
      721 tcggtcggcc tggtcgctct gactgccacc ctgctcagcg gttgtggagc gggtcagatc
      781 cactagttct agagcggccg cgccacggat gccaccacag gcactacaac ggagttcgcc
      841 acgtacatca ccacaa
//
