LOCUS       sequence-352006-        3948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC16A3_STOP
FEATURES             Location/Qualifiers
     source          1..3948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1505..1604)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1621..1640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1622..1640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1645..1661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1763..1782)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1774..2583
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1841..1860)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2730..3318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3219..3238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3472..3489
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3648..3675)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3767..3853)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3902..3924
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3916..3933
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3917..3933
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg aggagcagtg
      121 gtggacgagg gacctaccgg agtgaaggca ccagatggag gatggggatg ggccgtgctg
      181 ttcggatgct ttgtgatcac aggcttcagc tacgcctttc ccaaggccgt gtccgtgttc
      241 tttaaggagc tgatccagga gttcggcatc ggctactccg acaccgcctg gatcagctcc
      301 atcctgctgg ccatgctgta tggcacaggc cctctgtgca gcgtgtgcgt gaacaggttt
      361 ggatgcaggc cagtgatgct ggtgggagga ctgttcgcaa gcctgggaat ggtggcagca
      421 tctttttgta ggagcatcat ccaggtgtac ctgaccacag gcgtgatcac cggactggga
      481 ctggccctga acttccagcc atccctgatc atgctgaatc gctatttttc taagcggaga
      541 cccatggcaa acggactggc agcagcagga tctcccgtgt tcctgtgcgc actgagccct
      601 ctgggacagc tgctgcagga ccggtatggc tggagaggcg gctttctgat cctgggcggc
      661 ctgctgctga attgctgcgt gtgcgccgcc ctgatgcggc ccctggtggt gaccgcacag
      721 cctggatccg gaccacctag accatctagg cgcctgctgg acctgagcgt gttccgggat
      781 agaggctttg tgctgtacgc agtggcagca tccgtgatgg tgctgggcct gttcgtgcca
      841 cccgtgtttg tggtgtctta tgccaaggac ctgggcgtgc ctgataccaa ggccgccttc
      901 ctgctgacaa tcctgggctt catcgatatc tttgcaaggc cagcagcagg atttgtggca
      961 ggactgggca aggtgcgccc ttactccgtg tatctgttca gcttttccat gttctttaat
     1021 ggactggcag acctggcagg aagcaccgca ggcgattacg gcggcctggt ggtgttctgc
     1081 atcttctttg gcatctccta tggcatggtg ggcgccctgc agttcgaggt gctgatggcc
     1141 atcgtgggca cacacaagtt ttctagcgcc atcggactgg tgctgctgat ggaggcagtg
     1201 gccgtgctgg tgggacctcc aagcggaggc aagctgctgg atgcaaccca cgtgtacatg
     1261 tacgtgttca tcctggcagg agcagaggtg ctgacatcct ctctgatcct gctgctgggc
     1321 aacttctttt gtatccggaa gaagcccaag gagccacagc cagaggtggc agcagcagag
     1381 gaggagaagc tgcacaagcc acctgcagac tccggagtgg atctgaggga ggtggagcac
     1441 ttcctgaagg ccgagccaga gaagaatggc gaggtggtgc acacccccga gacatctgtg
     1501 taagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1561 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1621 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1681 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1741 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1801 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1861 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1921 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1981 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2041 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2101 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2161 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2221 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2281 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2341 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2401 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2461 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2521 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2581 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2641 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3361 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3421 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3481 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3541 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3601 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3661 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3721 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3781 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3841 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3901 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
