LOCUS       sequence-352111-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike A570D
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(137..164)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(256..342)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     391..413
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     405..422
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     406..422
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    438..537
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4357..4456)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4473..4492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4474..4492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4497..4513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4497..4513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4944..5735
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5828..6416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6317..6336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6570..6587
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
      121 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
      181 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
      241 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      301 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      361 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
      421 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      481 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatgt
      541 tcgtgttcct ggtgctgctg ccactggtga gctcccagtg cgtgaacctg accaccagaa
      601 cccagctgcc acctgcctac accaactcct tcaccagggg cgtgtactac ccagacaagg
      661 tgttcagatc cagcgtgctg cactctaccc aggacctgtt cctgccattc ttctctaacg
      721 tgacctggtt ccacgccatc cacgtgtctg gaaccaacgg caccaagaga ttcgacaacc
      781 cagtgctgcc attcaacgac ggcgtgtact tcgcttctac cgagaagtct aacatcatca
      841 ggggttggat cttcggaacc accctggact ctaagaccca gtctctgctg atcgtgaaca
      901 acgctaccaa cgtggtgatc aaggtgtgcg agttccagtt ctgcaacgac ccattcctgg
      961 gagtgtacta ccacaagaac aacaagtctt ggatggaaag cgagttcagg gtgtacagct
     1021 ctgctaacaa ctgcaccttc gagtacgtgt ctcagccttt cctgatggac ctggaaggaa
     1081 agcagggaaa cttcaagaac ctgagagagt tcgtgttcaa gaacatcgac ggttacttca
     1141 agatatacag caagcacacc ccaatcaacc tggtgaggga cctgccacag ggtttctctg
     1201 ctctggagcc cctggtggac ctgccaatcg gcatcaacat caccaggttc cagaccctgc
     1261 tggctctgca caggtcttac ctgaccccag gcgacagcag ctctggatgg accgccggcg
     1321 ccgctgctta ctacgtggga tacctgcagc caaggacctt cctgctgaag tacaacgaaa
     1381 acggcaccat caccgacgcc gtggactgcg ctctggaccc actgtctgag accaagtgca
     1441 ccctgaagtc tttcaccgtg gaaaagggaa tctaccagac cagcaacttc cgcgtgcagc
     1501 ccaccgagtc tatcgtgaga ttcccaaaca tcaccaacct gtgcccattc ggagaagtgt
     1561 tcaacgctac caggttcgcc agcgtgtacg cttggaacag gaagagaatc agcaactgcg
     1621 tggccgacta ctctgtgctg tacaactctg cctccttctc caccttcaag tgctacggcg
     1681 tgtccccaac caagctgaac gacctgtgct tcaccaacgt gtacgctgac tccttcgtga
     1741 tcagaggaga cgaagtgaga cagatcgctc ccggtcagac cggaaagatc gccgactaca
     1801 actacaagct gccagacgac ttcaccggtt gcgtgatcgc ttggaactct aacaacctgg
     1861 actctaaggt gggaggcaac tacaactacc tgtacaggct gttccgcaag agcaacctga
     1921 agcctttcga aagggacatc agcaccgaaa tctaccaggc tggatctacc ccatgcaacg
     1981 gagtggaagg attcaactgc tacttccctc tgcagtctta cggattccag ccaaccaacg
     2041 gagtgggata ccagccatac agagtggtgg tgctgtcttt cgagctgctg cacgctcctg
     2101 ctaccgtgtg cggaccaaag aagtctacca acctggtgaa gaacaagtgc gtgaacttca
     2161 acttcaacgg actgaccgga accggagtgc tgaccgaatc caacaagaag ttcctgcctt
     2221 tccagcagtt cggtagagac atcgacgaca ccaccgacgc tgtgagagac ccacagaccc
     2281 tggaaatcct ggacatcacc ccctgctctt tcggaggagt gagcgtgatc accccaggaa
     2341 ccaacacctc caaccaggtg gccgtgctgt accaggacgt gaactgcacc gaagtgccag
     2401 tggccatcca cgctgaccag ctgaccccaa cctggagagt gtacagcacc ggatctaacg
     2461 tgttccagac ccgtgccggc tgcctgatcg gagccgagca cgtgaacaac tcctacgaat
     2521 gcgacatccc aatcggagct ggcatctgcg cttcttacca gacccagacc aacagcccaa
     2581 gaagggccag atccgtggcc tctcagagca tcatcgccta caccatgagc ctgggcgctg
     2641 aaaacagcgt ggcttacagc aacaactcta tcgccatccc aaccaacttc accatctctg
     2701 tgaccaccga aatcctgcca gtgagcatga ccaagaccag cgtggactgc accatgtaca
     2761 tctgcggaga ctctaccgaa tgctctaacc tgctgctgca gtacggcagc ttctgcaccc
     2821 agctgaaccg cgctctgacc ggaatcgctg tggagcagga caagaacacc caggaagtgt
     2881 tcgctcaggt gaagcagata tacaagaccc cacctatcaa ggacttcgga ggattcaact
     2941 tctctcagat cctgcccgac ccatctaagc cctctaagag gtctttcatc gaagacctgc
     3001 tgttcaacaa ggtgaccctg gccgacgccg gtttcatcaa gcagtacggt gactgcctgg
     3061 gagacatcgc cgctagagac ctgatctgcg cccagaagtt caacggtctg accgtgctgc
     3121 caccactgct gaccgacgag atgatcgccc agtacacctc tgccctgctg gctggaacca
     3181 tcacctctgg ttggaccttc ggagctggcg ccgccctgca gatcccattc gctatgcaga
     3241 tggcctaccg tttcaacggt atcggagtga cccagaacgt gctgtacgaa aaccagaagc
     3301 tgatcgctaa ccagttcaac tctgctatcg gaaagatcca ggactccctg tcctctaccg
     3361 cttctgccct gggaaagctg caggacgtgg tgaaccagaa cgcccaggcc ctgaacaccc
     3421 tggtgaagca gctgtcttct aacttcggag ccatctcctc tgtgctgaac gacatcctgt
     3481 ctaggctgga caaggtggaa gccgaggtgc agatcgacag actgatcacc ggaagactgc
     3541 agtctctgca gacctacgtg acccagcagc tgatcagggc cgctgaaatc agagcttctg
     3601 ccaacctggc tgccaccaag atgtccgaat gcgtgctggg ccagtctaag agggtggact
     3661 tctgcggaaa gggataccac ctgatgtcct tcccacagtc tgctccacac ggagtggtgt
     3721 tcctgcacgt gacctacgtg cccgcccagg aaaagaactt caccaccgcc ccagctatct
     3781 gccacgacgg aaaggctcac ttccccaggg agggagtgtt cgtgtctaac ggaacccact
     3841 ggttcgtgac ccagaggaac ttctacgagc cacagatcat caccaccgac aacaccttcg
     3901 tgtctggaaa ctgcgacgtg gtgatcggaa tcgtgaacaa caccgtgtac gacccactgc
     3961 agccagaact ggactctttc aaggaggaac tggacaagta cttcaagaac cacacctctc
     4021 cagacgtgga cctgggagac atctccggaa tcaacgcctc tgtggtgaac atccagaagg
     4081 aaatcgacag actgaacgag gtggccaaga acctgaacga atctctgatc gacctgcagg
     4141 aactgggaaa gtacgagcag tacatcaagt ggccctggta catctggctg ggtttcatcg
     4201 ctggtctgat cgctatcgtg atggtgacca tcatgctgtg ctgcatgacc tcttgctgct
     4261 cttgcctgaa gggatgctgc tcttgcggat cttgctgcaa gttcgacgag gacgactccg
     4321 agcctgtgct gaagggtgtg aagctgcact acacctaccc aactttcttg tacaaagttg
     4381 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     4441 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     4501 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     4561 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     4621 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     4681 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     4741 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     4801 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     4861 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     4921 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     4981 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     5041 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     5101 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     5161 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     5221 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     5281 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     5341 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     5401 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     5461 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     5521 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     5581 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     5641 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     5701 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     5761 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6541 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6601 gcccaatac
//
