LOCUS       sequence-35215-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP TEV LIC cloning vector (1M)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..121)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(137..160)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc aagcttgtcg
       61 acggcgctcg aattcggatc cgttatccac ttccaatatt ggattggaag tacaggtttt
      121 cctcgatccc attagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
      181 cggcgttgat caccgcagta cgcacggcat accagaaagc ggacatctgc gggatgttcg
      241 gcatgatttc acctttctgg gcgttttcca tagtggcggc aatacgtgga tctttcgcca
      301 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
      361 ccagaccttc atcagtcagc agatagtttt cgaggaactc ttttgccagc tctttgttcg
      421 gactggcggc gttaatacct gcgctcagca cgccaacgaa cggtttggat ggttgaccct
      481 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
      541 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
      601 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
      661 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
      721 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
      781 ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct
      841 cttcccaggt
//
