LOCUS       sequence-352165-        4030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For phage display of antibody libraries by fusion to modified 
            geneIII..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ
  TITLE     Assembly of combinatorial antibody libraries on phage surfaces: the 
            gene III site.
  JOURNAL   Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.
  PUBMED    1896445
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1991-09-15"; volume: "88(18)"; pages: 
            "7978-82"
COMMENT     Sequence Label: pComb3
FEATURES             Location/Qualifiers
     source          1..4030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     46..111
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     125..141
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     126..142
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    924..945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(960..965,966..983,984..990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    998..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     1059..1124
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     complement(1185..1204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1186..1204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1211..1228)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1211..1227)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1220..1242)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1369..1824)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1506..1527)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1718..1737
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1851..1955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1956..2024,2025..2816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2174..2193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2987..3575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3476..3495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3729..3746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3863..3884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3899..3904,3905..3922,3923..3929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3937..3953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3961..3977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3961..3977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3998..4016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 caccgcggtg gcggccgcaa attctatttc aaggagacag tcataatgaa atacctattg
       61 cctacggcag ccgctggatt gttattactc gctgcccaac cagccatggc ccaggtgaaa
      121 ctgctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
      181 actagtggtg gcggtggctc tccattcgtt tgtgaatatc aaggccaatc gtctgacctg
      241 cctcaacctc ctgtcaatgc tggcggcggc tctggtggtg gttctggtgg cggctctgag
      301 ggtggtggct ctgagggtgg cggttctgag ggtggcggct ctgagggagg cggttccggt
      361 ggtggctctg gttccggtga ttttgattat gaaaagatgg caaacgctaa taagggggct
      421 atgaccgaaa atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct
      481 gtcgctactg attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct
      541 aatggtaatg gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt
      601 gacggtgata attcaccttt aatgaataat ttccgtcaat atttaccttc cctccctcaa
      661 tcggttgaat gtcgcccttt tgtctttggc gctggtaaac catatgaatt ttctattgat
      721 tgtgacaaaa taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt
      781 atgtatgtat tttctacgtt tgctaacata ctgcgtaata aggagtctta atcatgccag
      841 ttcttttggg tattccgtta ttatgctagc tagtaacacg acaggtttcc cgactggaaa
      901 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      961 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1021 gaattctaaa ctagctagtc gccaaggaga cagtcataat gaaataccta ttgcctacgg
     1081 cagccgctgg attgttatta ctcgctgccc aaccagccat ggccgagctc gtcagttcta
     1141 gagttaagcg gccgcaatcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
     1201 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1261 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1321 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg
     1381 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1441 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     1501 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     1561 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     1621 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     1681 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1741 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     1801 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3781 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3841 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     3901 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     3961 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     4021 aaaagctggc
//
