LOCUS       sequence-35225-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 TEV LIC cloning vector (2B-T)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             160..182
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             220..240
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(365..383)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      379..426
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat
      121 taagcggccg ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga
      181 gatatacata tgaaatcttc tcaccatcac catcaccatg aaaacctgta cttccaatcc
      241 aatattggaa gtggataacg gatccgcgat cgcggcgcgc cacctggtgg ccggccggta
      301 ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      361 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      421 tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat
      481 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
      541 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
      601 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
      661 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
      721 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
      781 taaactaccg cattaaagct tatcgatgat a
//
