LOCUS       sequence-35227-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 GST TEV LIC cloning vector (2G-T)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             166..188
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             208..225
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             235..885
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPP"
     primer_bind     846..868
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
       61 atacgactca ctatagggag accacaacgg tttccctcta gtgccggctc cggagagctc
      121 tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt ttaactttaa
      181 gaaggagata tacatatgaa atcttctcac catcaccatc accatggttc ttctatgtcc
      241 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      301 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      361 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      421 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
      481 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
      541 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
      601 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
      661 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
      721 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
      781 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
      841 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaa
//
