LOCUS       sequence-352512-        4466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of maturation protein and his-tagged coat protein dimer 
            from MS2 phage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Crone MA, Freemont PS
  TITLE     Simple Low-Cost Production of DNA MS2 Virus-Like Particles As 
            Molecular Diagnostic Controls.
  JOURNAL   GEN Biotechnol. 2022 Dec 1;1(6):496-503. doi: 
            10.1089/genbio.2022.0033. Epub 2022 Dec 21.
  PUBMED    36644571
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0033"; journalName: "GEN Biotechnol"; date: 
            "2022-12-1- 1"; volume: "1"; issue: "6"; pages: "496-503"
COMMENT     Sequence Label: pMC_MS2_DNA_VLP
FEATURES             Location/Qualifiers
     source          1..4466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     314..333
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        314..332
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    333..357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(366..371,372..389,390..395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             415..437
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1702..2088
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             2131..2148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2513..2531)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2527..2574
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2672..2689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2782..3370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2862..2881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3544..4338)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4339..4443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg aggtaatacg actcactata ggggaattgt gagcggataa caattcccct
      361 ctagatttac actttatgct tccggctcgt atgtttctag agaattcaaa taattttgtt
      421 taactttaag aaggagattt aaattggcta tcgctgtagg tagccggaat tccattccta
      481 ggaggtttga cctgtgcgag cttttagtac ccttgatagg gagaacgaaa ccttcgtccc
      541 ctccgttcgc gtttacgcgg acggtgagac tgaagataac tcattctctt taaaatatcg
      601 ttcgaactgg actcccggtc gttttaactc gactggggcc aaaacgaaac agtggcacta
      661 cccctctccg tattcacggg gggcgttaag tgtcacatcg atagatcaag gtgcctacaa
      721 gcgaagtggg tcatcgtggg gtcgcccgta cgaggagaaa gccggtttcg gcttctccct
      781 cgacgcacgc tcctgctaca gcctcttccc tgtaagccaa aacttgactt acatcgaagt
      841 gccgcagaac gttgcgaacc gggcgtcgac cgaagtcctg caaaaggtca cccagggtaa
      901 ttttaacctt ggtgttgctt tagcagaggc caggtcgaca gcctcacaac tcgcgacgca
      961 aaccattgcg ctcgtgaagg cgtacactgc cgctcgtcgc ggtaattggc gccaggcgct
     1021 ccgctacctt gccctaaacg aagatcgaaa gtttcgatca aaacacgtgg ccggcagatg
     1081 gttggagttg cagttcggtt ggttaccact aatgagtgat atccagggtg catatgagat
     1141 gcttacgaag gttcaccttc aagagtttct tcctatgaga gccgtacgtc aggtcggtac
     1201 taacatcaag ttagatggcc gtctgtcgta tccagctgca aacttccaga caacgtgcaa
     1261 catatcgcga cgtatcgtga tatggtttta cataaacgat gcacgtttgg catggttgtc
     1321 gtccctaggt atcttgaacc cactaggtat agtgtgggaa aaggtgcctt tctcattcgt
     1381 tgtcgactgg ctcctacctg taggtaacat gctcgagggc cttacggccc ccgtgggatg
     1441 ctcctacatg tcaggaacag ttactgacgt aataacgggt gagtccatca taagcgttga
     1501 cgctccctac gggtggactg tggagagaca gggcactgct aaggcccaaa tctcagccat
     1561 gcatcgaggg gtacaatccg tatggccaac aactggcgcg tacgtaaagt ctcctttctc
     1621 gatggtccat accttagatg cgttagcatt aatcaggcaa cggctctcta gatagagccc
     1681 tcaaccggag tttgaagcat ggcttctaac tttactcagt tcgttctcgt cgacaatggc
     1741 ggaactggcg acgtgactgt cgccccaagc aacttcgcta acggggtcgc tgaatggatc
     1801 agctctaact cgcgttcaca ggcttacaaa gtaacctgta gcgttcgtca gagctctgcg
     1861 cagaatcgca aatacaccat caaagtcgag gtgcctaaag tggcaaccca gactgttggt
     1921 ggtgtagagc ttcctgtagc cgcatggcgt tcgtacttaa atatggaact aaccattcca
     1981 attttcgcta cgaattccga ctgcgagctt attgttaagg caatgcaagg tcttctaaaa
     2041 gatggaaacc cgattccctc agcaatcgca gcaaactccg gcatctacgc taactttact
     2101 cagttcgttc tcgtcgacaa tggcggtacc catcaccatc accatcacgg taccggcgac
     2161 gtgactgtcg ccccaagcaa cttcgctaac ggggtcgctg aatggatcag ctctaactcg
     2221 cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa
     2281 tacaccatca aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg tgtagagctt
     2341 cctgtagccg catggcgttc gtacttaaat atggaactaa ccattccaat tttcgctacg
     2401 aattccgact gcgagcttat tgttaaggca atgcaaggtc ttctaaaaga tggaaacccg
     2461 attccctcag caatcgcagc aaactccggc atctactaat agacgccggc caccgctgag
     2521 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgtaattt
     2581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct
     2641 gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg
     2701 ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa
     2761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3181 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3541 ttattagaaa aattcatcca gcagacgata aaacgcaata cgctggctat ccggtgccgc
     3601 aatgccatac agcaccagaa aacgatccgc ccattcgccg cccagttctt ccgcaatatc
     3661 acgggtggcc agcgcaatat cctgataacg atccgccacg cccagacggc cgcaatcaat
     3721 aaagccgcta aaacggccat tttccaccat aatgttcggc aggcacgcat caccatgggt
     3781 caccaccaga tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca gctctgccgg
     3841 tgccaggccc tgatgttctt catccagatc atcctgatcc accaggcccg cttccatacg
     3901 ggtacgcgca cgttcaatac gatgtttcgc ctgatgatca aacggacagg tcgccgggtc
     3961 cagggtatgc agacgacgca tggcatccgc cataatgctc actttttctg ccggcgccag
     4021 atggctagac agcagatcct gacccggcac ttcgcccagc agcagccaat cacggcccgc
     4081 ttcggtcacc acatccagca ccgccgcaca cggaacaccg gtggtggcca gccagctcag
     4141 acgcgccgct tcatcctgca gctcgttcag cgcaccgctc agatcggttt tcacaaacag
     4201 caccggacga ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc caatggtctg
     4261 ctgcgcccaa tcatagccaa acagacgttc cacccacgct gccgggctac ccgcatgcag
     4321 gccatcctgt tcaatcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4441 gcgcacattt ccccgaaaag tgccac
//
