LOCUS       sequence-352588-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing optimized Cas9 and NAT marker. Compatible with 
            CTG-clade yeast species..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Strucko T, Andersen NL, Mahler MR, Martinez JL, Mortensen UH
  TITLE     A CRISPR/Cas9 method facilitates efficient oligo-mediated gene 
            editing in Debaryomyces hansenii.
  JOURNAL   Synth Biol (Oxf). 2021 Oct 12;6(1):ysab031. doi: 
            10.1093/synbio/ysab031. eCollection 2021.
  PUBMED    34746438
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-10-12"; volume: "6(1):ysab031. doi"; pages: " 
            10.1093/synbio/ysab031. eCollection 2021"
COMMENT     Sequence Label: pDIV490
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1613..1646)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(1853..1872)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1878..1897)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        1939..2030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2031..2099,2100..2891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2249..2268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3013..3601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3502..3521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3755..3772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3870..3887
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3871..3887
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4357..4390
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(4460..4493)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(4556..4753)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(6362..6550)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     6562..6580
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(6661..6726)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(6727..6747)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6751..6771)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6775..6795)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(6808..10867,1..44))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(6808..10867,1..44))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagttagtac caatatctaa accaatacta tactttttat ccatatgaca gattgtaatt
       61 tattttatta tgtttgtatt aatgaaagta ttgaatactg taaaaatata tattttattt
      121 atccattgta acagggatta ccacggtatt tatattcatc aattatttcc ttcaacaaaa
      181 agaaatgtct agctatctga atgtactact ctagcatacg attttttttt ttggttatca
      241 tcatcttatt acgtaacgcc attttcttga aaattctatt acgtaacgat gcttttgatg
      301 cgctatatac gcttgattcc ggtatgttac catataatac gctctaaaag tgtaaagtta
      361 tgttccggtg gtattgtggc caacagaaat aaaaaaccta ttacgtaatt gatcggaaac
      421 aaattgacca aaacgggtaa ttcacggttg attttgtaat tttatttctt ttggaaattg
      481 ttccattatt tctttttgtg gttaaaaacc gcttgcaacg gttttagaag gaccacgaga
      541 atacccacga tagctcgata ttacagtgaa gatcggaaaa acgatagcag cgctggtgtc
      601 aaaattaggt ttaattttct tggacctctt ggatctgtgg aactaccacg gatttgtgat
      661 ggatctctcg tagtctagag gtacgagttg gatactactc tagaacatat acgtacggta
      721 gtaaggacgc atgcgacgac aataattctg gatctattgt gggcacagtg ttggtacagt
      781 actggcccaa tgggaacgag taaaatgtcc tatgattctt aaagaccatg atacaggccc
      841 aaaggaggag aatgcacgcg atcgcacgca ttccgctagt gctgattatg atttgacgtt
      901 tatatacatg ttatgttaat atggtaaatt ttagatattt ggtaggagtg tcggtccttg
      961 aaatatatgt aatataccca ttataccaag gaaagattga tttaactata ttggattagc
     1021 agatatctaa tttgtttttt tgttcttaac ctctggtgag ttattatgat gtttttatat
     1081 tttatctttt ttaattaaaa gtcattattt agaacaaaat attaaaataa ttattaaagt
     1141 gtattagtat tcttaatgaa cgtcgggaag aacaaaattt taaacatttt gaagttaaac
     1201 atcactttta gtcctaaaag tttaaaaaac tgatattgat ggaaagaact aaagaagtgc
     1261 aaagagctca ccaaaaaacg tactgcgtat ctgttctttc tcattctgat attatccaaa
     1321 gatgttgaga tcatgaatgc tagtcttttt acactgaata tttttttaac gattttttaa
     1381 taattttgca atcatttaag aaaaaccaca aatggttttc caaaatttaa attgattttt
     1441 tagaaagtcc taaaaaatag aatcatacca aataaagaac aacttaaagt cactatgaca
     1501 aattcagaaa tacaatcgta tgatccgttc atggataaag ctgcaaagtc acaagcaagg
     1561 ctaactaagc ccattaaacc ctcagcgcgg ccgcatttaa atccccactt cagaagttcc
     1621 tatactttct agagaatagg aacttcacta tagagtcgaa taagggcgac accccataat
     1681 tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca
     1741 gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc
     1801 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt
     1861 gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg
     1921 ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc
     2041 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2101 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2161 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2221 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2281 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2341 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2401 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2461 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2521 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcga
     2581 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2641 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2701 cggctggctg gtttattgct gataaatccg gagccggtga gcgtggttct cgcggtatca
     2761 tcgcagcgct ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2821 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2881 agcattggta agcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2941 tcccttaacg tgagttacgc gcgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3181 agcccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3661 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3721 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3781 aggcgagagt agggaactgc caggcatcaa actaagcaga aggcccctga cggatggcct
     3841 ttttgcgttt ctacaaactc tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc
     3901 cccctgggcg gttctgataa cgagtaatcg ttaatccgca aataacgtaa aaacccgctt
     3961 cggcgggttt ttttatgggg ggagtttagg gaaagagcat ttgtcagaat atttaagggc
     4021 gcctgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga aaaaagcaac
     4081 gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt aaactattca
     4141 tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga gaattaagaa
     4201 aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt atacatgtca
     4261 acgataatac aaaatataat acaaactata agatgttatc agtatttatt atgcatttag
     4321 aataaatttt gtgtcgccct tattcgactc actatagaag ttcctattct ctagaaagta
     4381 taggaacttc acttcatttt atttaaattt gcggccgcgc tgaggtctta atggccacta
     4441 gtggatctga tatcacctaa taacttcgta tagcatacat tatacgaagt tatattaagg
     4501 gttctcgaga gctcgttttc gacactggat ggcggcgtta gtatcgaatc gacagcagta
     4561 tagcgaccag cattcacata cgattaacgc atgatattac tttctgcgca cttaacttcg
     4621 catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca tttgattaaa
     4681 atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa acaagaatct
     4741 ttttattgtc agtactgatt atggacaagg catagacatg tataaagctt gttctccgtc
     4801 tgaggctgta ccatcgtata aagctgtatc caatccacaa agtgtgaacc ccattcttcg
     4861 atatgcatgt atagctggtg cattaacatt ggtgacttct aaccataaat gtccagcccc
     4921 acgttcacgg gcaaattctg tagctaatcc cataagggct cgcccaacac cgtgcccacg
     4981 atgttctggg gcaacctcta tatcctcgac agtaagtctt cggttccatc cagaatagga
     5041 tacaactacg aaaccggcca aatcaccgtc gtctccataa gcgacaaatg ttcttgagtc
     5101 tgggtcaccg tcctcaccgt catcggattc atcgtcactt tcgtcatctg ggaatacttt
     5161 ggtcaaagga ggatctactg gtacttctcg caatgtaaac ccatccccag tagctgtaac
     5221 tctaaagacg gtgtcagttg tgaatgaccc gtcaagggct tcgatagctt cagcatcacc
     5281 aggtacagat gttcggtatc gataagcagt gtcatccaag gtggttccca tggttgtttg
     5341 ttttgtttgt agttaattga agttgtaatc aattgaaggg aaaaattctc tagaaatggg
     5401 ggccttgcgc tggctactta tatacttttc tccgccgggg caatccgctc cataagcgcg
     5461 tatccggcat atatttgccg cctacatcac aatgcactat gtttgcccgg gcgcacgccc
     5521 tccgctgaaa actccgagca agcactgtca tggtcaattt gtggcggttt ttcaccagga
     5581 agattagctc ttaacaatcc ataatgcatg gtttggtgac gtattatgcc atactaggca
     5641 atgaagggaa aatgcctgtc ccttcttcgt ttttggctat gtgttttcag aggctggccg
     5701 aggtcgccga gttgtggtgt aaaggtgtat gcaatgcaat tgtggagata attgtgcgtg
     5761 tgccaattga aatctatcca gccgggccac cgggcgccgc taatcgccgg gacttgggcc
     5821 gagcaaacca agattgaagt atacagaata tgccttcaaa ccttggcttt tatcatggtc
     5881 cacttgaatg tcttgtttgg tgcctttgat gaatgtgtct tgtggcttgt aggtgatttg
     5941 taatttgcgc acattttcct tcgtgtgagt caaaagtcac tctttcatgg aagatgacta
     6001 atgaaggagg agaagaaaat tccgatccac acttcaagct taagagctga ttacctacgt
     6061 cgaagtgata cacagcgact ctgacacgaa agccgcagtt ttttttaatg atgtcgacca
     6121 agttcgctcg ctcatgacac tggtgaaaaa ccgcacgaga cgaacaatgt aaatagatct
     6181 actgattcac gaggcttcga ggcgattttc tatactgctt tcatcaatcc tgtattacag
     6241 tgcgtagagc aaaggaaata aaaagagcaa acacctacta tagtgctttg ctggggctac
     6301 gattgcttgc tttccaagtt atattaaggg ttgtcgacct gcagcgttga atgcacgcga
     6361 tttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac
     6421 gcgtctgtac agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat
     6481 aactataaaa aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt
     6541 tagagcggat gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgactcg
     6601 acagaaattc gcccggaatt agcttggcgg caggtcgatg gatctctgca gtcattatca
     6661 cttgtcatcg tcatccttgt agtcgatgtc atgatcttta taatcaccgt catggtcttt
     6721 gtagtcaacc tttctctttt tcttaggatc caccttacgc tttttctttg gatcaacttt
     6781 tcttttcttc ttaggatcag ccccttcatc accacccaat tgagacaaat caattcttgt
     6841 ctcatacaat ccagtgattg attgatgaat taaggtggcg tctaagactt cttttgttga
     6901 ggtatatctt ttacgatcaa ttgttgtatc aaaatacttg aaagcagcag gtgctcctaa
     6961 attggtaagg gtaaataagt gaataatgtt ttctgcttgt tctctaattg gcttatctct
     7021 atgtttgtta taggctgaca aaactttatc taaatttgca tcagctaata tcactctttt
     7081 actgaattca ctaatttgtt ctataatctc atctaagtag tgtttgtgtt gttcaacaaa
     7141 taattgtttt tgctcgttat cttctggtga ccctttcaac ttttcatagt gtgaagctaa
     7201 atacaagaag ttaacatatt tggaaggcaa tgctaattca ttaccctttt gcaattctcc
     7261 agcagaagcc aacattcttt ttctcccatt ttctaattca aataaagaat atttaggcaa
     7321 tttgattatt aagtccttct ttacttcctt ataaccttta gcttccaaaa aatcaatagg
     7381 gttcttttca aaggaacttc tttccattat tgtaataccc aaaagttctt ttactgattt
     7441 aagcttttta cttttaccct tttcaacctt tgccacaacc aaaacagaat aggctacggt
     7501 tggtgagtcg aaaccaccat atttctttgg atcccaatcc ttttttctgg caatcaattt
     7561 atcactattt cttttaggca aaatactttc ttttgagaaa ccaccagttt gaacctctgt
     7621 tttctttaca atgttaactt gtggcattga taaaactttt ctaacagtag cgaaatcacg
     7681 ccctttgtcc caaactatct ctccagtctc accatttgtt tcaataagag gtctttttct
     7741 aatttcaccg tttgccaaag taatttctgt tttaaagaaa ttcataatgt tagagtaaaa
     7801 gaagtattta gctgtagctt taccaatttc ttgttctgat ttagcaatca tctttctaac
     7861 atcatagact ttgtaatcac catatacaaa ttcagattct aatttagggt atttcttgat
     7921 taaagcagtt ccaactacag cattcaaata tgcatcatga gcatgatgat aattattaat
     7981 ttctctaact ttatagaatt ggaaatcctt tctgaaatca gagacaagtt ttgattttaa
     8041 agtaatgact ttaacctcac gtattaactt atcattttcg tcatacttag tgttcatacg
     8101 ggaatctaaa atttgggcaa catgtttagt aatttgtcta gtttcgacca attgtctttt
     8161 aatgaacccg gctttatcta attcagaaag cccacctctt tctgctttag tcaaattatc
     8221 aaattttctt tgagtaatta atttagcatt caaaagttga cgccagtaat ttttcatctt
     8281 tttgacgact tcctcacttg ggacattatc acttttacct ctatttttat cacttctagt
     8341 taatacttta ttgtctatgg aatcatcttt caagaaagat tgtggaacta tatgatctac
     8401 atcataatct gaaagtctat ttatatctaa ttcctgatca acatacatat cacgtccatt
     8461 ttgaaggtag taaagatata atttttcatt ttgcaactgg gtattttcca ctgggtgttc
     8521 tttcaatatt tgactaccca attctttaat accctcttct attcttttca tacgttctct
     8581 actatttttc tgtccttttt gtgtagtttg attttctctt gccatctcaa tgacaatgtt
     8641 ttcaggttta tgtcttccca ttactttcac taattcatcc acaactttaa cagtttgtaa
     8701 tatccctttc ttaatagcag gagatccggc taaatttgca atatgttcat gtaatgaatc
     8761 accttgccca ctcacttggg ccttttggat gtcttcttta aatgtcaaac tatcgtcgtg
     8821 gatcaattgc atgaaatttc tattagcaaa tccgtcagat ttcaaaaagt ccaagatagt
     8881 tttaccactt tgtttatctc taattccatt gatcaattta cgagacaatc taccccatcc
     8941 agtataacgt cttcttttca actgtttcat cactttatcg tcaaacaaat gtgcataagt
     9001 cttcaaacgt tcttcaatca tctctctatc ttcgaataaa gttaaggtta aaacaatgtc
     9061 ttctaaaatg tcttcatttt cttcgttatc caagaaatct ttatctttga taattttaag
     9121 taaatcatgg taagttccta aactagcatt aaatctgtct tcaacaccac tgatttcaac
     9181 tgaatcaaaa cattcaattt tcttaaagta atcttctttt aattgtttaa cagtaacttt
     9241 tctatttgtt ttaaacaata aatcaactat tgcctttttc tgttctccag ataaaaaggc
     9301 tggcttacgc ataccttcag taacatattt aacctttgta agttcattgt aaacagtaaa
     9361 atattcgtat aacaaggaat gttttggtaa aactttttca tttggtaagt ttttatcgaa
     9421 attggtcatt ctttcaataa aactctgtgc tgatgctcct ttatcaacaa cctcttcaaa
     9481 attccaaggg gtaatagttt cttctgattt acgtgtcatc caggcaaatc ttgaattacc
     9541 tctagccaaa ggcccaacat agtatggaat tctaaaggtt aaaattttct cgattttttc
     9601 tctattatct tttaaaaatg gataaaaatc ttcttgtctt cttaaaattg catgcaactc
     9661 acccaaatga atttggtgag gtatactacc attatcaaaa gttctctgtt tacgtaacaa
     9721 atcttctctg ttcaatttga ccaaaagttc ctcagtccca tccatctttt ctaaaatagg
     9781 ttttataaat ttgtaaaatt cctcttggga ggcaccccca tcaatataac ctgcataccc
     9841 gtttttagat tgatcgaaaa agatttcttt atacttctct ggcaattgtt gtctcaccaa
     9901 agctttcaat aaagtcaaat cctgatggtg ttcatcataa cgtttgatca tagatgctga
     9961 taatggagct ttggttattt ctgtgttcac tctcaaaata tcagacaaca aaatagcatc
    10021 agataagttt ttagcagcta agaacaaatc tgcatattga tcaccaattt gagcaagtaa
    10081 attgtccaaa tcgtcgtcat aggtgtcttt tgataattgt aatttagcat cttcagctaa
    10141 atcaaaatta gatttaaaat ttggtgtcaa acccaatgat aatgcgatca agtttccaaa
    10201 taatccattc tttttttctc ctggcaattg ggcaatcaaa ttttccaaac gtctagattt
    10261 tgataatctg gctgataata tagctttggc atcgacacca ctagcattaa ttggattttc
    10321 ctcaaataat tggttgtagg tttggactaa ttgaataaat aatttatcca cgtcagaatt
    10381 atctgggttc aaatcacctt caatcaaaaa atgacctcta aatttaatca tatgggcaag
    10441 tgctaaatag attaatctaa ggtcagcttt atcagttgaa tctaccaatt tctttcttaa
    10501 atgataaatg gtaggatatt tttcatggta agccacttca tcaactatat tcccaaagat
    10561 tggatgtctc tcatgctttt tgtcttcttc aaccaaaaat gactcttcca atctatggaa
    10621 aaaggaatca tcaactttag ccatttcatt agaaaagatt tcttgtaaat aacatattct
    10681 attttttcta cgggtatatc ttcttcttgc tgttcttttt aaacgtgtag cctcggcagt
    10741 ttcaccggag tcgaacaaaa gagcaccgat aagattcttt tttattgagt gtctgtcagt
    10801 gttacctaac accttaaatt tctttgatgg aactttatat tcgtcagtga taactgccca
    10861 acccaca
//
